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Protein

Myosin-7

Gene

MYH7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.By similarity

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 185ATPBy similarity8

GO - Molecular functioni

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-7
Alternative name(s):
Myosin heavy chain 7
Myosin heavy chain slow isoform
Short name:
MyHC-slow
Myosin heavy chain, cardiac muscle beta isoform
Short name:
MyHC-beta
Gene namesi
Name:MYH7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002741751 – 1935Myosin-7Add BLAST1935

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei378PhosphothreonineBy similarity1
Modified residuei1137PhosphoserineBy similarity1
Modified residuei1269PhosphoserineBy similarity1
Modified residuei1282PhosphothreonineBy similarity1
Modified residuei1308PhosphotyrosineBy similarity1
Modified residuei1309PhosphothreonineBy similarity1
Modified residuei1510PhosphoserineBy similarity1
Modified residuei1513PhosphothreonineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9BE39.
PRIDEiQ9BE39.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with ECM29. Interacts (via C-terminus) with LRRC39.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000053217.

Structurei

Secondary structure

11935
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 15Combined sources6
Helixi20 – 27Combined sources8
Turni33 – 35Combined sources3
Beta strandi36 – 41Combined sources6
Turni42 – 44Combined sources3
Beta strandi45 – 55Combined sources11
Beta strandi58 – 65Combined sources8
Beta strandi68 – 72Combined sources5
Helixi73 – 75Combined sources3
Helixi82 – 84Combined sources3
Helixi90 – 92Combined sources3
Helixi98 – 110Combined sources13
Beta strandi115 – 118Combined sources4
Beta strandi121 – 125Combined sources5
Helixi136 – 142Combined sources7
Turni147 – 149Combined sources3
Helixi154 – 168Combined sources15
Beta strandi172 – 177Combined sources6
Helixi184 – 199Combined sources16
Helixi216 – 231Combined sources16
Beta strandi244 – 252Combined sources9
Beta strandi254 – 256Combined sources3
Beta strandi258 – 266Combined sources9
Helixi270 – 273Combined sources4
Helixi284 – 290Combined sources7
Helixi295 – 300Combined sources6
Helixi307 – 309Combined sources3
Helixi311 – 313Combined sources3
Helixi325 – 338Combined sources14
Helixi343 – 359Combined sources17
Beta strandi364 – 366Combined sources3
Beta strandi368 – 371Combined sources4
Beta strandi373 – 376Combined sources4
Helixi379 – 388Combined sources10
Helixi392 – 400Combined sources9
Beta strandi403 – 406Combined sources4
Beta strandi407 – 410Combined sources4
Helixi417 – 447Combined sources31
Beta strandi455 – 461Combined sources7
Helixi473 – 494Combined sources22
Helixi496 – 503Combined sources8
Helixi518 – 525Combined sources8
Helixi530 – 537Combined sources8
Helixi545 – 556Combined sources12
Turni557 – 559Combined sources3
Beta strandi576 – 581Combined sources6
Beta strandi584 – 588Combined sources5
Helixi593 – 598Combined sources6
Helixi603 – 610Combined sources8
Helixi615 – 620Combined sources6
Helixi647 – 663Combined sources17
Beta strandi665 – 673Combined sources9
Helixi686 – 696Combined sources11
Helixi698 – 707Combined sources10
Beta strandi711 – 714Combined sources4
Helixi715 – 722Combined sources8
Helixi723 – 725Combined sources3
Helixi727 – 729Combined sources3
Helixi738 – 747Combined sources10
Beta strandi749 – 751Combined sources3
Helixi753 – 755Combined sources3
Beta strandi756 – 758Combined sources3
Beta strandi760 – 765Combined sources6
Helixi767 – 808Combined sources42

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5N69X-ray2.45A/B1-828[»]
5N6AX-ray3.10A1-828[»]
ProteinModelPortaliQ9BE39.
SMRiQ9BE39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 81Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST50
Domaini85 – 778Myosin motorPROSITE-ProRule annotationAdd BLAST694
Domaini781 – 810IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni655 – 677Actin-bindingBy similarityAdd BLAST23
Regioni757 – 771Actin-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili839 – 1935Sequence analysisAdd BLAST1097

Domaini

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Four skip residues (Skip1: Thr-1188, Skip2: Glu-1385, Skip3: Glu-1582 and Skip4: Gly-1807) introduce discontinuities in the coiled-coil heptad repeats. The first three skip residues are structurally comparable and induce a unique local relaxation of the coiled-coil superhelical pitch and the fourth skip residue lies within a highly flexible molecular hinge that is necessary for myosin incorporation in the bare zone of sarcomeres.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ9BE39.
KOiK17751.

Family and domain databases

Gene3Di2.30.30.360. 1 hit.
InterProiView protein in InterPro
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR008989. Myosin_S1_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
PRINTSiPR00193. MYOSINHEAVY.
SMARTiView protein in SMART
SM00242. MYSc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51844. SH3_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BE39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDAEMAAFG EAAPYLRKSE KERLEAQTRP FDLKKDVFVP DDKEEFVKAT
60 70 80 90 100
ILSREGGKVT AETEHGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA
110 120 130 140 150
VLYNLKERYA SWMIYTYSGL FCVTINPYKW LPVYNAEVVA AYRGKKRSEA
160 170 180 190 200
PPHIFSISDN AYQYMLTDRE NQSILITGES GAGKTVNTKR VIQYFAVIAA
210 220 230 240 250
IGDRSKKEQA TGKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI
260 270 280 290 300
HFGATGKLAS ADIETYLLEK SRVIFQLKAE RDYHIFYQIL SNKKPELLDM
310 320 330 340 350
LLITNNPYDY AFISQGETTV ASIDDAEELM ATDNAFDVLG FTTEEKNSMY
360 370 380 390 400
KLTGAIMHFG NMKFKLKQRE EQAEPDGTEE ADKSAYLMGL NSADLLKGLC
410 420 430 440 450
HPRVKVGNEY VTKGQNVQQV VYAKGALAKA VYERMFNWMV TRINATLETK
460 470 480 490 500
QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE
510 520 530 540 550
YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA
560 570 580 590 600
KLFDNHLGKS SNFQKPRNIK GKPEAHFSLI HYAGTVDYNI IGWLQKNKDP
610 620 630 640 650
LNETVVDLYK KSSLKMLSSL FANYAGFDTP IEKGKGKAKK GSSFQTVSAL
660 670 680 690 700
HRENLNKLMT NLRSTHPHFV RCIIPNETKS PGVIDNPLVM HQLRCNGVLE
710 720 730 740 750
GIRICRKGFP NRILYGDFRQ RYRILNPAAI PEGQFIDSRK GAEKLLGSLD
760 770 780 790 800
IDHNQYKFGH TKVFFKAGLL GLLEEMRDER LSRIITRIQA QSRGVLSRME
810 820 830 840 850
FKKLLERRDS LLIIQWNIRA FMGVKNWPWM KLYFKIKPLL KSAETEKEIA
860 870 880 890 900
LMKEEFGRLK EALEKSEARR KELEEKMVSL LQEKNDLQLQ VQAEQDNLAD
910 920 930 940 950
AEERCDQLIK NKIQLEAKVK EMTERLEDEE EMNAELTAKK RKLEDECSEL
960 970 980 990 1000
KRDIDDLELT LAKVEKEKHA TENKVKNLTE EMAGLDEIIA KLTKEKKALQ
1010 1020 1030 1040 1050
EAHQQALDDL QAEEDKVNTL TKAKVKLEQH VDDLEGSLEQ EKKVRMDLER
1060 1070 1080 1090 1100
AKRKLEGDLK LTQESIMDLE NDKQQLDERL KKKDFELNAL NARIEDEQAL
1110 1120 1130 1140 1150
GSQLQKKLKE LQARIEELEE ELEAERTARA KVEKLRSDLS RELEEISERL
1160 1170 1180 1190 1200
EEAGGATSVQ IEMNKKREAE FQKMRRDLEE ATLQHEATAA ALRKKHADSV
1210 1220 1230 1240 1250
AELSEQIDNL QRVKQKLEKE KSEFKLELDD VTSNMEQIIK AKANLEKMCR
1260 1270 1280 1290 1300
TLEDQMNEHR SKAEETQRSV NDLTSQRAKL QTENGELSRQ LDEKEALISQ
1310 1320 1330 1340 1350
LTRGKLTYTQ QLEDLKRQLE EEVKAKNALA HALQSARHDC DLLREQYEEE
1360 1370 1380 1390 1400
TEAKAELQRV LSKANSEVAQ WRTKYETDAI QRTEELEEAK KKLAQRLQDA
1410 1420 1430 1440 1450
EEAVEAVNAK CSSLEKTKHR LQNEIEDLMV DVERSNAAAA ALDKKQRNFD
1460 1470 1480 1490 1500
KILAEWKQKY EESQSELESS QKEARSLSTE LFKLKNAYEE SLEHLETFKR
1510 1520 1530 1540 1550
ENKNLQEEIS DLTEQLGSSG KTIHELEKVR KQLEAEKLEL QSALEEAEAS
1560 1570 1580 1590 1600
LEQEEGKILR AQLEFNQIKA EMERKLAEKD EEMEQAKRNH LRVVDSLQTS
1610 1620 1630 1640 1650
LDAETRSRNE ALRVKKKMEG DLNEMEIQLS HANRLAAEAQ KQVKSLQSLL
1660 1670 1680 1690 1700
KDTQIQLDDA VRANDDLKEN IAIVERRNNL LQAELEELRA VVEQTERSRK
1710 1720 1730 1740 1750
LAEQELIETS ERVQLLHSQN TSLINQKKKM EADLSQLQTE VEEAVQECRN
1760 1770 1780 1790 1800
AEEKAKKAIT DAAMMAEELK KEQDTSAHLE RMKKNMEQTI KDLQHRLDEA
1810 1820 1830 1840 1850
EQIALKGGKK QLQKLEARVR ELENELEAEQ KRNAESVKGM RKSERRIKEL
1860 1870 1880 1890 1900
TYQTEEDRKN LLRLQDLVDK LQLKVKAYKR QAEEAEEQAN TNLSKFRKVQ
1910 1920 1930
HELDEAEERA DIAESQVNKL RAKSRDIGTK GLNEE
Length:1,935
Mass (Da):223,229
Last modified:June 1, 2001 - v1
Checksum:iDEA05200BCF42557
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti754 – 755NQ → QN AA sequence (PubMed:5058224).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059400 mRNA. Translation: BAB40922.1.
RefSeqiNP_777152.1. NM_174727.1.
UniGeneiBt.107108.
Bt.60957.

Genome annotation databases

GeneIDi282714.
KEGGibta:282714.

Similar proteinsi

Entry informationi

Entry nameiMYH7_BOVIN
AccessioniPrimary (citable) accession number: Q9BE39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-7 (MYO7).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families