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Protein

CD40 ligand

Gene

CD40LG

Organism
Macaca nemestrina (Pig-tailed macaque)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.By similarity
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
CD40 ligand
Short name:
CD40-L
Alternative name(s):
Tumor necrosis factor ligand superfamily member 5
CD_antigen: CD154
Cleaved into the following 2 chains:
Gene namesi
Name:CD40LG
Synonyms:CD40L, TNFSF5
OrganismiMacaca nemestrina (Pig-tailed macaque)
Taxonomic identifieri9545 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity
  • Cell surface By similarity
CD40 ligand, soluble form :
  • Secreted By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini44 – 240197ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240CD40 ligand, membrane formPRO_0000034488Add
BLAST
Chaini113 – 240128CD40 ligand, soluble formBy similarityPRO_0000034489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi173 ↔ 197Sequence analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei112 – 1132CleavageBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer. Interacts with CD28.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9BDM7.
SMRiQ9BDM7. Positions 108-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG079629.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BDM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETYNQPSP RSAATGLPVR MKIFMYLLTI FLITQMIGSA LFAVYLHRRL
60 70 80 90 100
DKIEDERNLH EDFVFMKTIQ RCNTGERSLS LLDCEEIKSQ FEGFVKDIML
110 120 130 140 150
NKEEKKKENS FEMQKVLQWA EKGYYTMSNN LVTLENGKQL TVKRQGLYYI
160 170 180 190 200
YAQVTFCSNR EASSQAPFIA SLCLKSPGRF ERILLRAANT HSSTKPCGQQ
210 220 230 240
SIHLGGVFEL QPGASVFVNV TDPSQVSHGT GFTSFGLLKL
Length:240
Mass (Da):27,273
Last modified:June 1, 2001 - v1
Checksum:iBAE6AA0213CAEDEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF344853 mRNA. Translation: AAK37613.1.
RefSeqiNP_001292848.1. NM_001305919.1.

Genome annotation databases

GeneIDi105481370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF344853 mRNA. Translation: AAK37613.1.
RefSeqiNP_001292848.1. NM_001305919.1.

3D structure databases

ProteinModelPortaliQ9BDM7.
SMRiQ9BDM7. Positions 108-240.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi105481370.

Organism-specific databases

CTDi959.

Phylogenomic databases

HOVERGENiHBG079629.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules."
    Villinger F.J., Bostik P., Mayne A.E., King C.L., Genain C.P., Weiss W.R., Ansari A.A.
    Immunogenetics 53:315-328(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lymphocyte.

Entry informationi

Entry nameiCD40L_MACNE
AccessioniPrimary (citable) accession number: Q9BDM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.