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Q9AXT8 (CMT1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase 1

EC=2.1.1.37
Alternative name(s):
Chromomethylase 1
DNA cytosine methyltransferase MET2a
Zea methyltransferase2
Short name=Zmet2
Gene names
Name:MET2A
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length912 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the CpXpG methylation and in gene silencing.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 BAH domain.

Contains 1 chromo domain.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 912912DNA (cytosine-5)-methyltransferase 1
PRO_0000246694

Regions

Domain184 – 309126BAH
Domain341 – 872532SAM-dependent MTase C5-type
Domain441 – 50464Chromo
Compositional bias31 – 355Poly-Glu
Compositional bias73 – 10735Glu-rich

Sites

Active site5171 By similarity

Experimental info

Mutagenesis834 – 91279Missing in zmet2-m1::Mu; loss of activity. Ref.1

Secondary structure

........................................................................................................................... 912
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9AXT8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C697C45AE152FB4D

FASTA912101,046
        10         20         30         40         50         60 
MAPSSPSPAA PTRVSGRKRA AKAEEIHQNK EEEEEVAAAS SAKRSRKAAS SGKKPKSPPK 

        70         80         90        100        110        120 
QAKPGRKKKG DAEMKEPVED DVCAEEPDEE ELAMGEEEAE EQAMQEEVVA VAAGSPGKKR 

       130        140        150        160        170        180 
VGRRNAAAAA GDHEPEFIGS PVAADEARSN WPKRYGRSTA AKKPDEEEEL KARCHYRSAK 

       190        200        210        220        230        240 
VDNVVYCLGD DVYVKAGENE ADYIGRITEF FEGTDQCHYF TCRWFFRAED TVINSLVSIS 

       250        260        270        280        290        300 
VDGHKHDPRR VFLSEEKNDN VLDCIISKVK IVHVDPNMDP KAKAQLIESC DLYYDMSYSV 

       310        320        330        340        350        360 
AYSTFANISS ENGQSGSDTA SGISSDDVDL ETSSSMPTRT ATLLDLYSGC GGMSTGLCLG 

       370        380        390        400        410        420 
AALSGLKLET RWAVDFNSFA CQSLKYNHPQ TEVRNEKADE FLALLKEWAV LCKKYVQDVD 

       430        440        450        460        470        480 
SNLASSEDQA DEDSPLDKDE FVVEKLVGIC YGGSDRENGI YFKVQWEGYG PEEDTWEPID 

       490        500        510        520        530        540 
NLSDCPQKIR EFVQEGHKRK ILPLPGDVDV ICGGPPCQGI SGFNRYRNRD EPLKDEKNKQ 

       550        560        570        580        590        600 
MVTFMDIVAY LKPKYVLMEN VVDILKFADG YLGKYALSCL VAMKYQARLG MMVAGCYGLP 

       610        620        630        640        650        660 
QFRMRVFLWG ALSSMVLPKY PLPTYDVVVR GGAPNAFSQC MVAYDETQKP SLKKALLLGD 

       670        680        690        700        710        720 
AISDLPKVQN HQPNDVMEYG GSPKTEFQRY IRLSRKDMLD WSFGEGAGPD EGKLLDHQPL 

       730        740        750        760        770        780 
RLNNDDYERV QQIPVKKGAN FRDLKGVRVG ANNIVEWDPE IERVKLSSGK PLVPDYAMSF 

       790        800        810        820        830        840 
IKGKSLKPFG RLWWDETVPT VVTRAEPHNQ VIIHPTQARV LTIRENARLQ GFPDYYRLFG 

       850        860        870        880        890        900 
PIKEKYIQVG NAVAVPVARA LGYCLGQAYL GESEGSDPLY QLPPSFTSVG GRTAGQARAS 

       910 
PVGTPAGEVV EQ 

« Hide

References

[1]"Maize chromomethylase Zea methyltransferase2 is required for CpNpG methylation."
Papa C.M., Springer N.M., Muszynski M.G., Meeley R., Kaeppler S.M.
Plant Cell 13:1919-1928(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF 834-ASP--GLN-912.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF243043 mRNA. Translation: AAK11516.1.
RefSeqNP_001104978.1. NM_001111508.1.
UniGeneZm.587.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4FSXX-ray3.20A/B130-912[»]
4FT2X-ray3.20A/B130-912[»]
4FT4X-ray2.70A/B130-912[»]
ProteinModelPortalQ9AXT8.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE4907. M.ZmaIIA.

Proteomic databases

PRIDEQ9AXT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID541827.
KEGGzma:541827.

Organism-specific databases

GrameneQ9AXT8.

Phylogenomic databases

HOGENOMHOG000082844.
KOK00558.

Family and domain databases

InterProIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SMARTSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMSSF54160. SSF54160. 1 hit.
PROSITEPS51038. BAH. 1 hit.
PS00094. C5_MTASE_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCMT1_MAIZE
AccessionPrimary (citable) accession number: Q9AXT8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references