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Q9AXT8

- CMT1_MAIZE

UniProt

Q9AXT8 - CMT1_MAIZE

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Protein

DNA (cytosine-5)-methyltransferase 1

Gene

MET2A

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the CpXpG methylation and in gene silencing.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei517 – 5171PROSITE-ProRule annotation

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  3. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi4907. M.ZmaIIA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
Alternative name(s):
Chromomethylase 1
DNA cytosine methyltransferase MET2a
Zea methyltransferase2
Short name:
Zmet2
Gene namesi
Name:MET2A
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ9AXT8.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi834 – 91279Missing in zmet2-m1::Mu; loss of activity. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912DNA (cytosine-5)-methyltransferase 1PRO_0000246694Add
BLAST

Proteomic databases

PRIDEiQ9AXT8.

Structurei

Secondary structure

1
912
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi144 – 1507
Helixi152 – 1543
Beta strandi172 – 1754
Beta strandi177 – 1815
Beta strandi184 – 1874
Beta strandi191 – 1944
Beta strandi203 – 21311
Beta strandi218 – 22710
Helixi228 – 2303
Helixi234 – 2385
Beta strandi250 – 26112
Helixi262 – 2643
Beta strandi265 – 2684
Beta strandi271 – 2733
Helixi280 – 28910
Beta strandi291 – 2999
Helixi301 – 3033
Beta strandi304 – 3074
Beta strandi339 – 3468
Helixi352 – 36413
Beta strandi366 – 37611
Helixi378 – 38710
Beta strandi391 – 3966
Helixi398 – 41417
Beta strandi443 – 45210
Beta strandi454 – 4563
Beta strandi458 – 4669
Helixi471 – 4733
Beta strandi475 – 4795
Helixi480 – 4823
Helixi486 – 49914
Beta strandi509 – 5135
Beta strandi519 – 5213
Helixi522 – 5265
Turni532 – 5343
Helixi539 – 55113
Beta strandi554 – 5618
Helixi562 – 5654
Helixi567 – 5704
Helixi571 – 58212
Beta strandi586 – 5938
Helixi594 – 5974
Beta strandi604 – 6118
Helixi635 – 6373
Helixi658 – 6625
Beta strandi675 – 6773
Helixi686 – 6916
Turni695 – 6995
Beta strandi703 – 7064
Helixi724 – 7329
Helixi741 – 7433
Beta strandi747 – 7493
Helixi751 – 7533
Beta strandi755 – 7573
Beta strandi759 – 7613
Beta strandi769 – 7735
Helixi775 – 7795
Helixi780 – 7834
Beta strandi798 – 8003
Beta strandi807 – 8137
Beta strandi815 – 8206
Helixi823 – 8297
Helixi842 – 85110
Helixi855 – 87016
Beta strandi878 – 8803
Beta strandi884 – 8874

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FSXX-ray3.20A/B130-912[»]
4FT2X-ray3.20A/B130-912[»]
4FT4X-ray2.70A/B130-912[»]
ProteinModelPortaliQ9AXT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini184 – 309126BAHPROSITE-ProRule annotationAdd
BLAST
Domaini341 – 872532SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST
Domaini441 – 50464ChromoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 355Poly-Glu
Compositional biasi73 – 10735Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 1 chromo domain.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000082844.
KOiK00558.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
PS00094. C5_MTASE_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AXT8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSSPSPAA PTRVSGRKRA AKAEEIHQNK EEEEEVAAAS SAKRSRKAAS
60 70 80 90 100
SGKKPKSPPK QAKPGRKKKG DAEMKEPVED DVCAEEPDEE ELAMGEEEAE
110 120 130 140 150
EQAMQEEVVA VAAGSPGKKR VGRRNAAAAA GDHEPEFIGS PVAADEARSN
160 170 180 190 200
WPKRYGRSTA AKKPDEEEEL KARCHYRSAK VDNVVYCLGD DVYVKAGENE
210 220 230 240 250
ADYIGRITEF FEGTDQCHYF TCRWFFRAED TVINSLVSIS VDGHKHDPRR
260 270 280 290 300
VFLSEEKNDN VLDCIISKVK IVHVDPNMDP KAKAQLIESC DLYYDMSYSV
310 320 330 340 350
AYSTFANISS ENGQSGSDTA SGISSDDVDL ETSSSMPTRT ATLLDLYSGC
360 370 380 390 400
GGMSTGLCLG AALSGLKLET RWAVDFNSFA CQSLKYNHPQ TEVRNEKADE
410 420 430 440 450
FLALLKEWAV LCKKYVQDVD SNLASSEDQA DEDSPLDKDE FVVEKLVGIC
460 470 480 490 500
YGGSDRENGI YFKVQWEGYG PEEDTWEPID NLSDCPQKIR EFVQEGHKRK
510 520 530 540 550
ILPLPGDVDV ICGGPPCQGI SGFNRYRNRD EPLKDEKNKQ MVTFMDIVAY
560 570 580 590 600
LKPKYVLMEN VVDILKFADG YLGKYALSCL VAMKYQARLG MMVAGCYGLP
610 620 630 640 650
QFRMRVFLWG ALSSMVLPKY PLPTYDVVVR GGAPNAFSQC MVAYDETQKP
660 670 680 690 700
SLKKALLLGD AISDLPKVQN HQPNDVMEYG GSPKTEFQRY IRLSRKDMLD
710 720 730 740 750
WSFGEGAGPD EGKLLDHQPL RLNNDDYERV QQIPVKKGAN FRDLKGVRVG
760 770 780 790 800
ANNIVEWDPE IERVKLSSGK PLVPDYAMSF IKGKSLKPFG RLWWDETVPT
810 820 830 840 850
VVTRAEPHNQ VIIHPTQARV LTIRENARLQ GFPDYYRLFG PIKEKYIQVG
860 870 880 890 900
NAVAVPVARA LGYCLGQAYL GESEGSDPLY QLPPSFTSVG GRTAGQARAS
910
PVGTPAGEVV EQ
Length:912
Mass (Da):101,046
Last modified:June 1, 2001 - v1
Checksum:iC697C45AE152FB4D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243043 mRNA. Translation: AAK11516.1.
RefSeqiNP_001104978.1. NM_001111508.1.
UniGeneiZm.587.

Genome annotation databases

GeneIDi541827.
KEGGizma:541827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243043 mRNA. Translation: AAK11516.1 .
RefSeqi NP_001104978.1. NM_001111508.1.
UniGenei Zm.587.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4FSX X-ray 3.20 A/B 130-912 [» ]
4FT2 X-ray 3.20 A/B 130-912 [» ]
4FT4 X-ray 2.70 A/B 130-912 [» ]
ProteinModelPortali Q9AXT8.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 4907. M.ZmaIIA.

Proteomic databases

PRIDEi Q9AXT8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 541827.
KEGGi zma:541827.

Organism-specific databases

Gramenei Q9AXT8.

Phylogenomic databases

HOGENOMi HOG000082844.
KOi K00558.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEi PS51038. BAH. 1 hit.
PS00094. C5_MTASE_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Maize chromomethylase Zea methyltransferase2 is required for CpNpG methylation."
    Papa C.M., Springer N.M., Muszynski M.G., Meeley R., Kaeppler S.M.
    Plant Cell 13:1919-1928(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF 834-ASP--GLN-912.

Entry informationi

Entry nameiCMT1_MAIZE
AccessioniPrimary (citable) accession number: Q9AXT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3