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Q9AXT8

- CMT1_MAIZE

UniProt

Q9AXT8 - CMT1_MAIZE

Protein

DNA (cytosine-5)-methyltransferase 1

Gene

MET2A

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Involved in the CpXpG methylation and in gene silencing.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei517 – 5171PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    3. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi4907. M.ZmaIIA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
    Alternative name(s):
    Chromomethylase 1
    DNA cytosine methyltransferase MET2a
    Zea methyltransferase2
    Short name:
    Zmet2
    Gene namesi
    Name:MET2A
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ9AXT8.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi834 – 91279Missing in zmet2-m1::Mu; loss of activity. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 912912DNA (cytosine-5)-methyltransferase 1PRO_0000246694Add
    BLAST

    Proteomic databases

    PRIDEiQ9AXT8.

    Structurei

    Secondary structure

    1
    912
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi144 – 1507
    Helixi152 – 1543
    Beta strandi172 – 1754
    Beta strandi177 – 1815
    Beta strandi184 – 1874
    Beta strandi191 – 1944
    Beta strandi203 – 21311
    Beta strandi218 – 22710
    Helixi228 – 2303
    Helixi234 – 2385
    Beta strandi250 – 26112
    Helixi262 – 2643
    Beta strandi265 – 2684
    Beta strandi271 – 2733
    Helixi280 – 28910
    Beta strandi291 – 2999
    Helixi301 – 3033
    Beta strandi304 – 3074
    Beta strandi339 – 3468
    Helixi352 – 36413
    Beta strandi366 – 37611
    Helixi378 – 38710
    Beta strandi391 – 3966
    Helixi398 – 41417
    Beta strandi443 – 45210
    Beta strandi454 – 4563
    Beta strandi458 – 4669
    Helixi471 – 4733
    Beta strandi475 – 4795
    Helixi480 – 4823
    Helixi486 – 49914
    Beta strandi509 – 5135
    Beta strandi519 – 5213
    Helixi522 – 5265
    Turni532 – 5343
    Helixi539 – 55113
    Beta strandi554 – 5618
    Helixi562 – 5654
    Helixi567 – 5704
    Helixi571 – 58212
    Beta strandi586 – 5938
    Helixi594 – 5974
    Beta strandi604 – 6118
    Helixi635 – 6373
    Helixi658 – 6625
    Beta strandi675 – 6773
    Helixi686 – 6916
    Turni695 – 6995
    Beta strandi703 – 7064
    Helixi724 – 7329
    Helixi741 – 7433
    Beta strandi747 – 7493
    Helixi751 – 7533
    Beta strandi755 – 7573
    Beta strandi759 – 7613
    Beta strandi769 – 7735
    Helixi775 – 7795
    Helixi780 – 7834
    Beta strandi798 – 8003
    Beta strandi807 – 8137
    Beta strandi815 – 8206
    Helixi823 – 8297
    Helixi842 – 85110
    Helixi855 – 87016
    Beta strandi878 – 8803
    Beta strandi884 – 8874

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4FSXX-ray3.20A/B130-912[»]
    4FT2X-ray3.20A/B130-912[»]
    4FT4X-ray2.70A/B130-912[»]
    ProteinModelPortaliQ9AXT8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini184 – 309126BAHPROSITE-ProRule annotationAdd
    BLAST
    Domaini341 – 872532SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini441 – 50464ChromoPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi31 – 355Poly-Glu
    Compositional biasi73 – 10735Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 BAH domain.PROSITE-ProRule annotation
    Contains 1 chromo domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000082844.
    KOiK00558.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEiPS51038. BAH. 1 hit.
    PS00094. C5_MTASE_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AXT8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPSSPSPAA PTRVSGRKRA AKAEEIHQNK EEEEEVAAAS SAKRSRKAAS    50
    SGKKPKSPPK QAKPGRKKKG DAEMKEPVED DVCAEEPDEE ELAMGEEEAE 100
    EQAMQEEVVA VAAGSPGKKR VGRRNAAAAA GDHEPEFIGS PVAADEARSN 150
    WPKRYGRSTA AKKPDEEEEL KARCHYRSAK VDNVVYCLGD DVYVKAGENE 200
    ADYIGRITEF FEGTDQCHYF TCRWFFRAED TVINSLVSIS VDGHKHDPRR 250
    VFLSEEKNDN VLDCIISKVK IVHVDPNMDP KAKAQLIESC DLYYDMSYSV 300
    AYSTFANISS ENGQSGSDTA SGISSDDVDL ETSSSMPTRT ATLLDLYSGC 350
    GGMSTGLCLG AALSGLKLET RWAVDFNSFA CQSLKYNHPQ TEVRNEKADE 400
    FLALLKEWAV LCKKYVQDVD SNLASSEDQA DEDSPLDKDE FVVEKLVGIC 450
    YGGSDRENGI YFKVQWEGYG PEEDTWEPID NLSDCPQKIR EFVQEGHKRK 500
    ILPLPGDVDV ICGGPPCQGI SGFNRYRNRD EPLKDEKNKQ MVTFMDIVAY 550
    LKPKYVLMEN VVDILKFADG YLGKYALSCL VAMKYQARLG MMVAGCYGLP 600
    QFRMRVFLWG ALSSMVLPKY PLPTYDVVVR GGAPNAFSQC MVAYDETQKP 650
    SLKKALLLGD AISDLPKVQN HQPNDVMEYG GSPKTEFQRY IRLSRKDMLD 700
    WSFGEGAGPD EGKLLDHQPL RLNNDDYERV QQIPVKKGAN FRDLKGVRVG 750
    ANNIVEWDPE IERVKLSSGK PLVPDYAMSF IKGKSLKPFG RLWWDETVPT 800
    VVTRAEPHNQ VIIHPTQARV LTIRENARLQ GFPDYYRLFG PIKEKYIQVG 850
    NAVAVPVARA LGYCLGQAYL GESEGSDPLY QLPPSFTSVG GRTAGQARAS 900
    PVGTPAGEVV EQ 912
    Length:912
    Mass (Da):101,046
    Last modified:June 1, 2001 - v1
    Checksum:iC697C45AE152FB4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF243043 mRNA. Translation: AAK11516.1.
    RefSeqiNP_001104978.1. NM_001111508.1.
    UniGeneiZm.587.

    Genome annotation databases

    GeneIDi541827.
    KEGGizma:541827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF243043 mRNA. Translation: AAK11516.1 .
    RefSeqi NP_001104978.1. NM_001111508.1.
    UniGenei Zm.587.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4FSX X-ray 3.20 A/B 130-912 [» ]
    4FT2 X-ray 3.20 A/B 130-912 [» ]
    4FT4 X-ray 2.70 A/B 130-912 [» ]
    ProteinModelPortali Q9AXT8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 4907. M.ZmaIIA.

    Proteomic databases

    PRIDEi Q9AXT8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 541827.
    KEGGi zma:541827.

    Organism-specific databases

    Gramenei Q9AXT8.

    Phylogenomic databases

    HOGENOMi HOG000082844.
    KOi K00558.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEi PS51038. BAH. 1 hit.
    PS00094. C5_MTASE_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Maize chromomethylase Zea methyltransferase2 is required for CpNpG methylation."
      Papa C.M., Springer N.M., Muszynski M.G., Meeley R., Kaeppler S.M.
      Plant Cell 13:1919-1928(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF 834-ASP--GLN-912.

    Entry informationi

    Entry nameiCMT1_MAIZE
    AccessioniPrimary (citable) accession number: Q9AXT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3