Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA (cytosine-5)-methyltransferase 1

Gene

MET2A

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the CpXpG methylation and in gene silencing.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei517PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi4907. M.ZmaIIA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
Alternative name(s):
Chromomethylase 1
DNA cytosine methyltransferase MET2a
Zea methyltransferase2
Short name:
Zmet2
Gene namesi
Name:MET2A
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi834 – 912Missing in zmet2-m1::Mu; loss of activity. 1 PublicationAdd BLAST79

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002466941 – 912DNA (cytosine-5)-methyltransferase 1Add BLAST912

Proteomic databases

PaxDbiQ9AXT8.
PRIDEiQ9AXT8.

PTM databases

iPTMnetiQ9AXT8.

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G025592_P01.

Structurei

Secondary structure

1912
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 150Combined sources7
Helixi152 – 154Combined sources3
Beta strandi172 – 175Combined sources4
Beta strandi177 – 181Combined sources5
Beta strandi184 – 187Combined sources4
Beta strandi191 – 194Combined sources4
Beta strandi203 – 213Combined sources11
Beta strandi218 – 227Combined sources10
Helixi228 – 230Combined sources3
Helixi234 – 238Combined sources5
Beta strandi250 – 261Combined sources12
Helixi262 – 264Combined sources3
Beta strandi265 – 268Combined sources4
Beta strandi271 – 273Combined sources3
Helixi280 – 289Combined sources10
Beta strandi291 – 299Combined sources9
Helixi301 – 303Combined sources3
Beta strandi304 – 307Combined sources4
Beta strandi339 – 346Combined sources8
Helixi352 – 364Combined sources13
Beta strandi366 – 376Combined sources11
Helixi378 – 387Combined sources10
Beta strandi391 – 396Combined sources6
Helixi398 – 414Combined sources17
Beta strandi443 – 452Combined sources10
Beta strandi454 – 456Combined sources3
Beta strandi458 – 466Combined sources9
Helixi471 – 473Combined sources3
Beta strandi475 – 479Combined sources5
Helixi480 – 482Combined sources3
Helixi486 – 499Combined sources14
Beta strandi509 – 513Combined sources5
Beta strandi519 – 521Combined sources3
Helixi522 – 526Combined sources5
Turni532 – 534Combined sources3
Helixi539 – 551Combined sources13
Beta strandi554 – 561Combined sources8
Helixi562 – 565Combined sources4
Helixi567 – 570Combined sources4
Helixi571 – 582Combined sources12
Beta strandi586 – 593Combined sources8
Helixi594 – 597Combined sources4
Beta strandi604 – 611Combined sources8
Helixi635 – 637Combined sources3
Helixi658 – 662Combined sources5
Beta strandi675 – 677Combined sources3
Helixi686 – 691Combined sources6
Turni695 – 699Combined sources5
Beta strandi703 – 706Combined sources4
Helixi724 – 732Combined sources9
Helixi741 – 743Combined sources3
Beta strandi747 – 749Combined sources3
Helixi751 – 753Combined sources3
Beta strandi755 – 757Combined sources3
Beta strandi759 – 761Combined sources3
Beta strandi769 – 773Combined sources5
Helixi775 – 779Combined sources5
Helixi780 – 783Combined sources4
Beta strandi798 – 800Combined sources3
Beta strandi807 – 813Combined sources7
Beta strandi815 – 820Combined sources6
Helixi823 – 829Combined sources7
Helixi842 – 851Combined sources10
Helixi855 – 870Combined sources16
Beta strandi878 – 880Combined sources3
Beta strandi884 – 887Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FSXX-ray3.20A/B130-912[»]
4FT2X-ray3.20A/B130-912[»]
4FT4X-ray2.70A/B130-912[»]
ProteinModelPortaliQ9AXT8.
SMRiQ9AXT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 309BAHPROSITE-ProRule annotationAdd BLAST126
Domaini341 – 872SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST532
Domaini441 – 504ChromoPROSITE-ProRule annotationAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 35Poly-Glu5
Compositional biasi73 – 107Glu-richAdd BLAST35

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 1 chromo domain.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH5K. Eukaryota.
COG0270. LUCA.
HOGENOMiHOG000082844.
KOiK00558.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
PS00094. C5_MTASE_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AXT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSSPSPAA PTRVSGRKRA AKAEEIHQNK EEEEEVAAAS SAKRSRKAAS
60 70 80 90 100
SGKKPKSPPK QAKPGRKKKG DAEMKEPVED DVCAEEPDEE ELAMGEEEAE
110 120 130 140 150
EQAMQEEVVA VAAGSPGKKR VGRRNAAAAA GDHEPEFIGS PVAADEARSN
160 170 180 190 200
WPKRYGRSTA AKKPDEEEEL KARCHYRSAK VDNVVYCLGD DVYVKAGENE
210 220 230 240 250
ADYIGRITEF FEGTDQCHYF TCRWFFRAED TVINSLVSIS VDGHKHDPRR
260 270 280 290 300
VFLSEEKNDN VLDCIISKVK IVHVDPNMDP KAKAQLIESC DLYYDMSYSV
310 320 330 340 350
AYSTFANISS ENGQSGSDTA SGISSDDVDL ETSSSMPTRT ATLLDLYSGC
360 370 380 390 400
GGMSTGLCLG AALSGLKLET RWAVDFNSFA CQSLKYNHPQ TEVRNEKADE
410 420 430 440 450
FLALLKEWAV LCKKYVQDVD SNLASSEDQA DEDSPLDKDE FVVEKLVGIC
460 470 480 490 500
YGGSDRENGI YFKVQWEGYG PEEDTWEPID NLSDCPQKIR EFVQEGHKRK
510 520 530 540 550
ILPLPGDVDV ICGGPPCQGI SGFNRYRNRD EPLKDEKNKQ MVTFMDIVAY
560 570 580 590 600
LKPKYVLMEN VVDILKFADG YLGKYALSCL VAMKYQARLG MMVAGCYGLP
610 620 630 640 650
QFRMRVFLWG ALSSMVLPKY PLPTYDVVVR GGAPNAFSQC MVAYDETQKP
660 670 680 690 700
SLKKALLLGD AISDLPKVQN HQPNDVMEYG GSPKTEFQRY IRLSRKDMLD
710 720 730 740 750
WSFGEGAGPD EGKLLDHQPL RLNNDDYERV QQIPVKKGAN FRDLKGVRVG
760 770 780 790 800
ANNIVEWDPE IERVKLSSGK PLVPDYAMSF IKGKSLKPFG RLWWDETVPT
810 820 830 840 850
VVTRAEPHNQ VIIHPTQARV LTIRENARLQ GFPDYYRLFG PIKEKYIQVG
860 870 880 890 900
NAVAVPVARA LGYCLGQAYL GESEGSDPLY QLPPSFTSVG GRTAGQARAS
910
PVGTPAGEVV EQ
Length:912
Mass (Da):101,046
Last modified:June 1, 2001 - v1
Checksum:iC697C45AE152FB4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243043 mRNA. Translation: AAK11516.1.
RefSeqiNP_001104978.1. NM_001111508.1.
UniGeneiZm.163182.
Zm.587.

Genome annotation databases

GeneIDi541827.
KEGGizma:541827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243043 mRNA. Translation: AAK11516.1.
RefSeqiNP_001104978.1. NM_001111508.1.
UniGeneiZm.163182.
Zm.587.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FSXX-ray3.20A/B130-912[»]
4FT2X-ray3.20A/B130-912[»]
4FT4X-ray2.70A/B130-912[»]
ProteinModelPortaliQ9AXT8.
SMRiQ9AXT8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G025592_P01.

Protein family/group databases

REBASEi4907. M.ZmaIIA.

PTM databases

iPTMnetiQ9AXT8.

Proteomic databases

PaxDbiQ9AXT8.
PRIDEiQ9AXT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi541827.
KEGGizma:541827.

Phylogenomic databases

eggNOGiENOG410IH5K. Eukaryota.
COG0270. LUCA.
HOGENOMiHOG000082844.
KOiK00558.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
PS00094. C5_MTASE_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMT1_MAIZE
AccessioniPrimary (citable) accession number: Q9AXT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.