Reviewed, UniProtKB/Swiss-Prot Q9AXT8 (CMT1_MAIZE)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase 1
    EC=2.1.1.37
Alternative name(s):
    Chromomethylase 1
    Zea methyltransferase2
      Short name=Zmet2
    DNA cytosine methyltransferase MET2a

Gene names

Name:

MET2A

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the CpXpG methylation and in gene silencing.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 BAH domain. Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 912	912	DNA (cytosine-5)-methyltransferase 1		PRO_0000246694
Regions
	Domain	184 – 309	126	BAH
	Domain	441 – 504	64	Chromo
	Compositional bias	31 – 35	5	Poly-Glu
	Compositional bias	73 – 107	35	Glu-rich
Experimental info
	Mutagenesis	834 – 912	79	Missing in zmet2-m1::Mu; loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9AXT8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C697C45AE152FB4D	FASTA	912	101,046
	10 20 30 40 50 60 MAPSSPSPAA PTRVSGRKRA AKAEEIHQNK EEEEEVAAAS SAKRSRKAAS SGKKPKSPPK 70 80 90 100 110 120 QAKPGRKKKG DAEMKEPVED DVCAEEPDEE ELAMGEEEAE EQAMQEEVVA VAAGSPGKKR 130 140 150 160 170 180 VGRRNAAAAA GDHEPEFIGS PVAADEARSN WPKRYGRSTA AKKPDEEEEL KARCHYRSAK 190 200 210 220 230 240 VDNVVYCLGD DVYVKAGENE ADYIGRITEF FEGTDQCHYF TCRWFFRAED TVINSLVSIS 250 260 270 280 290 300 VDGHKHDPRR VFLSEEKNDN VLDCIISKVK IVHVDPNMDP KAKAQLIESC DLYYDMSYSV 310 320 330 340 350 360 AYSTFANISS ENGQSGSDTA SGISSDDVDL ETSSSMPTRT ATLLDLYSGC GGMSTGLCLG 370 380 390 400 410 420 AALSGLKLET RWAVDFNSFA CQSLKYNHPQ TEVRNEKADE FLALLKEWAV LCKKYVQDVD 430 440 450 460 470 480 SNLASSEDQA DEDSPLDKDE FVVEKLVGIC YGGSDRENGI YFKVQWEGYG PEEDTWEPID 490 500 510 520 530 540 NLSDCPQKIR EFVQEGHKRK ILPLPGDVDV ICGGPPCQGI SGFNRYRNRD EPLKDEKNKQ 550 560 570 580 590 600 MVTFMDIVAY LKPKYVLMEN VVDILKFADG YLGKYALSCL VAMKYQARLG MMVAGCYGLP 610 620 630 640 650 660 QFRMRVFLWG ALSSMVLPKY PLPTYDVVVR GGAPNAFSQC MVAYDETQKP SLKKALLLGD 670 680 690 700 710 720 AISDLPKVQN HQPNDVMEYG GSPKTEFQRY IRLSRKDMLD WSFGEGAGPD EGKLLDHQPL 730 740 750 760 770 780 RLNNDDYERV QQIPVKKGAN FRDLKGVRVG ANNIVEWDPE IERVKLSSGK PLVPDYAMSF 790 800 810 820 830 840 IKGKSLKPFG RLWWDETVPT VVTRAEPHNQ VIIHPTQARV LTIRENARLQ GFPDYYRLFG 850 860 870 880 890 900 PIKEKYIQVG NAVAVPVARA LGYCLGQAYL GESEGSDPLY QLPPSFTSVG GRTAGQARAS 910 PVGTPAGEVV EQ

References

[1]	"Maize chromomethylase Zea methyltransferase2 is required for CpNpG methylation." Papa C.M., Springer N.M., Muszynski M.G., Meeley R., Kaeppler S.M. Plant Cell 13:1919-1928(2001) [PubMed: 11487702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF 834-ASP--GLN-912.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF243043 mRNA. Translation: AAK11516.1.
RefSeq	NP_001104978.1.
UniGene	Zm.587
3D structure databases
SMR	Q9AXT8. Positions 185-310, 342-867.
ModBase	Search...
Protein family/group databases
REBASE	4907. M.ZmaIIA.
Genome annotation databases
GeneID	541827.
KEGG	zma:541827.
Organism-specific databases
Gramene	Q9AXT8.
Enzyme and pathway databases
BRENDA	2.1.1.37. 289.
Family and domain databases
InterPro	IPR001025. BAH_dom. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR000953. Chromodomain. IPR016197. Chromodomain-like. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF01426. BAH. 1 hit. PF00385. Chromo. 1 hit. PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view]
PROSITE	PS51038. BAH. 1 hit. PS00094. C5_MTASE_1. 1 hit. PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMT1_MAIZE

Accession

Primary (citable) accession number: Q9AXT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)