Q9AXQ8 (DHYS_DIACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyhypusine synthase
EC=2.5.1.46

Gene names

Name:

DHS

Organism

Dianthus caryophyllus (Carnation) (Clove pink)

Taxonomic identifier

3570 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.
Catalytic activity	[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.
Cofactor	NAD.
Pathway	Protein modification; eIF5A hypusination.
Sequence similarities	Belongs to the deoxyhypusine synthase family.

Ontologies

Keywords
Biological process	Hypusine biosynthesis
Ligand	NAD
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	deoxyhypusine biosynthetic process from spermidine Inferred from electronic annotation. Source: EC peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	deoxyhypusine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Deoxyhypusine synthase		PRO_0000134475
Regions
	Nucleotide binding	105 – 109	5	NAD By similarity
	Nucleotide binding	131 – 133	3	NAD By similarity
	Nucleotide binding	315 – 316	2	NAD By similarity
	Nucleotide binding	349 – 350	2	NAD By similarity
	Region	136 – 137	2	Spermidine binding By similarity
	Region	321 – 323	3	Spermidine binding By similarity
	Region	330 – 336	7	Spermidine binding By similarity
Sites
	Active site	336	1	Nucleophile By similarity
	Binding site	137	1	NAD By similarity
	Binding site	243	1	NAD By similarity
	Binding site	248	1	Spermidine By similarity
	Binding site	290	1	NAD; via amide nitrogen By similarity
	Binding site	295	1	Spermidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AXQ8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D410956C043B647C	FASTA	373	41,126
	10 20 30 40 50 60 MEDANHDSVA SAHSAAFKKS ENLEGKSVKI EGYDFNQGVN YSKLLQSFAS NGFQASNLGD 70 80 90 100 110 120 AIEVVNHMLD WSLADEAPVD DCSEEERDPK FRESVKCKVF LGFTSNLISS GVRDTIRYLV 130 140 150 160 170 180 QHHMVDVIVT TTGGIEEDLI KGRSIKCLAP TFKGDFALPG AQLRSKGLNR IGNLLVPNDN 190 200 210 220 230 240 YCKFEDWIIP ILDKMLEEQI SEKILWTPSK LIGRLGREIN DESSYLYWAF KNNIPVFCPG 250 260 270 280 290 300 LTDGSLGDML YFHSFRNPGL IVDVVQDIRA VNGEAVHAAP RKTGMIILGG GLPKHHICNA 310 320 330 340 350 360 NMMRNGADYA VFINTAEEFD GSDSGARPDE AISWGKISGS AKTVKVHCDA TIAFPLLVAE 370 TFAAKREKER KSC

References

[1]

"Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato." Wang T.-W., Lu L., Wang D., Thompson J.E. J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF296080 mRNA. Translation: AAG53644.1.
3D structure databases
ProteinModelPortal	Q9AXQ8.
SMR	Q9AXQ8. Positions 10-367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00354.
Family and domain databases
Gene3D	3.40.910.10. 1 hit.
InterPro	IPR002773. Deoxyhypusine_synthase. [Graphical view]
PANTHER	PTHR11703. PTHR11703. 1 hit.
Pfam	PF01916. DS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00321. dhys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHYS_DIACA

Accession

Primary (citable) accession number: Q9AXQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program