Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9AXQ8 (DHYS_DIACA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyhypusine synthase

EC=2.5.1.46
Gene names
Name:DHS
OrganismDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifier3570 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeDianthus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.

Catalytic activity

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactor

NAD.

Pathway

Protein modification; eIF5A hypusination.

Sequence similarities

Belongs to the deoxyhypusine synthase family.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   LigandNAD
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processdeoxyhypusine biosynthetic process from spermidine

Inferred from electronic annotation. Source: UniProtKB-EC

peptidyl-lysine modification to hypusine

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondeoxyhypusine synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Deoxyhypusine synthase
PRO_0000134475

Regions

Nucleotide binding105 – 1095NAD By similarity
Nucleotide binding131 – 1333NAD By similarity
Nucleotide binding315 – 3162NAD By similarity
Nucleotide binding349 – 3502NAD By similarity
Region136 – 1372Spermidine binding By similarity
Region321 – 3233Spermidine binding By similarity
Region330 – 3367Spermidine binding By similarity

Sites

Active site3361Nucleophile By similarity
Binding site1371NAD By similarity
Binding site2431NAD By similarity
Binding site2481Spermidine By similarity
Binding site2901NAD; via amide nitrogen By similarity
Binding site2951Spermidine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9AXQ8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D410956C043B647C

FASTA37341,126
        10         20         30         40         50         60 
MEDANHDSVA SAHSAAFKKS ENLEGKSVKI EGYDFNQGVN YSKLLQSFAS NGFQASNLGD 

        70         80         90        100        110        120 
AIEVVNHMLD WSLADEAPVD DCSEEERDPK FRESVKCKVF LGFTSNLISS GVRDTIRYLV 

       130        140        150        160        170        180 
QHHMVDVIVT TTGGIEEDLI KGRSIKCLAP TFKGDFALPG AQLRSKGLNR IGNLLVPNDN 

       190        200        210        220        230        240 
YCKFEDWIIP ILDKMLEEQI SEKILWTPSK LIGRLGREIN DESSYLYWAF KNNIPVFCPG 

       250        260        270        280        290        300 
LTDGSLGDML YFHSFRNPGL IVDVVQDIRA VNGEAVHAAP RKTGMIILGG GLPKHHICNA 

       310        320        330        340        350        360 
NMMRNGADYA VFINTAEEFD GSDSGARPDE AISWGKISGS AKTVKVHCDA TIAFPLLVAE 

       370 
TFAAKREKER KSC 

« Hide

References

[1]"Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
Wang T.-W., Lu L., Wang D., Thompson J.E.
J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Flower.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF296080 mRNA. Translation: AAG53644.1.

3D structure databases

ProteinModelPortalQ9AXQ8.
SMRQ9AXQ8. Positions 10-367.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00354.

Family and domain databases

Gene3D3.40.910.10. 1 hit.
InterProIPR002773. Deoxyhypusine_synthase.
[Graphical view]
PANTHERPTHR11703. PTHR11703. 1 hit.
PfamPF01916. DS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00321. dhys. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDHYS_DIACA
AccessionPrimary (citable) accession number: Q9AXQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways