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Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137NADBy similarity1
Binding sitei243NADBy similarity1
Binding sitei248SpermidineBy similarity1
Binding sitei290NAD; via amide nitrogenBy similarity1
Binding sitei295SpermidineBy similarity1
Active sitei336NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 109NADBy similarity5
Nucleotide bindingi131 – 133NADBy similarity3
Nucleotide bindingi315 – 316NADBy similarity2
Nucleotide bindingi349 – 350NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
OrganismiDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifieri3570 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeCaryophylleaeDianthus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344751 – 373Deoxyhypusine synthaseAdd BLAST373

Structurei

3D structure databases

ProteinModelPortaliQ9AXQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 137Spermidine bindingBy similarity2
Regioni321 – 323Spermidine bindingBy similarity3
Regioni330 – 336Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9AXQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDANHDSVA SAHSAAFKKS ENLEGKSVKI EGYDFNQGVN YSKLLQSFAS
60 70 80 90 100
NGFQASNLGD AIEVVNHMLD WSLADEAPVD DCSEEERDPK FRESVKCKVF
110 120 130 140 150
LGFTSNLISS GVRDTIRYLV QHHMVDVIVT TTGGIEEDLI KGRSIKCLAP
160 170 180 190 200
TFKGDFALPG AQLRSKGLNR IGNLLVPNDN YCKFEDWIIP ILDKMLEEQI
210 220 230 240 250
SEKILWTPSK LIGRLGREIN DESSYLYWAF KNNIPVFCPG LTDGSLGDML
260 270 280 290 300
YFHSFRNPGL IVDVVQDIRA VNGEAVHAAP RKTGMIILGG GLPKHHICNA
310 320 330 340 350
NMMRNGADYA VFINTAEEFD GSDSGARPDE AISWGKISGS AKTVKVHCDA
360 370
TIAFPLLVAE TFAAKREKER KSC
Length:373
Mass (Da):41,126
Last modified:June 1, 2001 - v1
Checksum:iD410956C043B647C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296080 mRNA. Translation: AAG53644.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296080 mRNA. Translation: AAG53644.1.

3D structure databases

ProteinModelPortaliQ9AXQ8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_DIACA
AccessioniPrimary (citable) accession number: Q9AXQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: June 1, 2001
Last modified: October 5, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.