Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9AXQ8

- DHYS_DIACA

UniProt

Q9AXQ8 - DHYS_DIACA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei137 – 1371NADBy similarity
Binding sitei243 – 2431NADBy similarity
Binding sitei248 – 2481SpermidineBy similarity
Binding sitei290 – 2901NAD; via amide nitrogenBy similarity
Binding sitei295 – 2951SpermidineBy similarity
Active sitei336 – 3361NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1095NADBy similarity
Nucleotide bindingi131 – 1333NADBy similarity
Nucleotide bindingi315 – 3162NADBy similarity
Nucleotide bindingi349 – 3502NADBy similarity

GO - Molecular functioni

  1. deoxyhypusine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. deoxyhypusine biosynthetic process from spermidine Source: UniProtKB-EC
  2. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
OrganismiDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifieri3570 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeDianthus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Deoxyhypusine synthasePRO_0000134475Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9AXQ8.
SMRiQ9AXQ8. Positions 10-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1372Spermidine bindingBy similarity
Regioni321 – 3233Spermidine bindingBy similarity
Regioni330 – 3367Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9AXQ8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDANHDSVA SAHSAAFKKS ENLEGKSVKI EGYDFNQGVN YSKLLQSFAS
60 70 80 90 100
NGFQASNLGD AIEVVNHMLD WSLADEAPVD DCSEEERDPK FRESVKCKVF
110 120 130 140 150
LGFTSNLISS GVRDTIRYLV QHHMVDVIVT TTGGIEEDLI KGRSIKCLAP
160 170 180 190 200
TFKGDFALPG AQLRSKGLNR IGNLLVPNDN YCKFEDWIIP ILDKMLEEQI
210 220 230 240 250
SEKILWTPSK LIGRLGREIN DESSYLYWAF KNNIPVFCPG LTDGSLGDML
260 270 280 290 300
YFHSFRNPGL IVDVVQDIRA VNGEAVHAAP RKTGMIILGG GLPKHHICNA
310 320 330 340 350
NMMRNGADYA VFINTAEEFD GSDSGARPDE AISWGKISGS AKTVKVHCDA
360 370
TIAFPLLVAE TFAAKREKER KSC
Length:373
Mass (Da):41,126
Last modified:June 1, 2001 - v1
Checksum:iD410956C043B647C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF296080 mRNA. Translation: AAG53644.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF296080 mRNA. Translation: AAG53644.1 .

3D structure databases

ProteinModelPortali Q9AXQ8.
SMRi Q9AXQ8. Positions 10-367.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00354 .

Family and domain databases

Gene3Di 3.40.910.10. 1 hit.
InterProi IPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view ]
PANTHERi PTHR11703. PTHR11703. 1 hit.
Pfami PF01916. DS. 1 hit.
[Graphical view ]
SUPFAMi SSF52467. SSF52467. 1 hit.
TIGRFAMsi TIGR00321. dhys. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
    Wang T.-W., Lu L., Wang D., Thompson J.E.
    J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Flower.

Entry informationi

Entry nameiDHYS_DIACA
AccessioniPrimary (citable) accession number: Q9AXQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: June 1, 2001
Last modified: October 1, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3