Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9AXQ8

- DHYS_DIACA

UniProt

Q9AXQ8 - DHYS_DIACA

Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.By similarity

    Catalytic activityi

    [eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

    Cofactori

    NAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei137 – 1371NADBy similarity
    Binding sitei243 – 2431NADBy similarity
    Binding sitei248 – 2481SpermidineBy similarity
    Binding sitei290 – 2901NAD; via amide nitrogenBy similarity
    Binding sitei295 – 2951SpermidineBy similarity
    Active sitei336 – 3361NucleophileBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi105 – 1095NADBy similarity
    Nucleotide bindingi131 – 1333NADBy similarity
    Nucleotide bindingi315 – 3162NADBy similarity
    Nucleotide bindingi349 – 3502NADBy similarity

    GO - Molecular functioni

    1. deoxyhypusine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyhypusine biosynthetic process from spermidine Source: UniProtKB-EC
    2. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Hypusine biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00354.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyhypusine synthase (EC:2.5.1.46)
    Gene namesi
    Name:DHS
    OrganismiDianthus caryophyllus (Carnation) (Clove pink)
    Taxonomic identifieri3570 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeDianthus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Deoxyhypusine synthasePRO_0000134475Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AXQ8.
    SMRiQ9AXQ8. Positions 10-367.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 1372Spermidine bindingBy similarity
    Regioni321 – 3233Spermidine bindingBy similarity
    Regioni330 – 3367Spermidine bindingBy similarity

    Sequence similaritiesi

    Belongs to the deoxyhypusine synthase family.Curated

    Family and domain databases

    Gene3Di3.40.910.10. 1 hit.
    InterProiIPR002773. Deoxyhypusine_synthase.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    [Graphical view]
    PANTHERiPTHR11703. PTHR11703. 1 hit.
    PfamiPF01916. DS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    TIGRFAMsiTIGR00321. dhys. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9AXQ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDANHDSVA SAHSAAFKKS ENLEGKSVKI EGYDFNQGVN YSKLLQSFAS    50
    NGFQASNLGD AIEVVNHMLD WSLADEAPVD DCSEEERDPK FRESVKCKVF 100
    LGFTSNLISS GVRDTIRYLV QHHMVDVIVT TTGGIEEDLI KGRSIKCLAP 150
    TFKGDFALPG AQLRSKGLNR IGNLLVPNDN YCKFEDWIIP ILDKMLEEQI 200
    SEKILWTPSK LIGRLGREIN DESSYLYWAF KNNIPVFCPG LTDGSLGDML 250
    YFHSFRNPGL IVDVVQDIRA VNGEAVHAAP RKTGMIILGG GLPKHHICNA 300
    NMMRNGADYA VFINTAEEFD GSDSGARPDE AISWGKISGS AKTVKVHCDA 350
    TIAFPLLVAE TFAAKREKER KSC 373
    Length:373
    Mass (Da):41,126
    Last modified:June 1, 2001 - v1
    Checksum:iD410956C043B647C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296080 mRNA. Translation: AAG53644.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296080 mRNA. Translation: AAG53644.1 .

    3D structure databases

    ProteinModelPortali Q9AXQ8.
    SMRi Q9AXQ8. Positions 10-367.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00354 .

    Family and domain databases

    Gene3Di 3.40.910.10. 1 hit.
    InterProi IPR002773. Deoxyhypusine_synthase.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    [Graphical view ]
    PANTHERi PTHR11703. PTHR11703. 1 hit.
    Pfami PF01916. DS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    TIGRFAMsi TIGR00321. dhys. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
      Wang T.-W., Lu L., Wang D., Thompson J.E.
      J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Flower.

    Entry informationi

    Entry nameiDHYS_DIACA
    AccessioniPrimary (citable) accession number: Q9AXQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 21, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3