ID   T10H_TAXCU              Reviewed;         497 AA.
AC   Q9AXM6;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-MAR-2009, entry version 39.
DE   RecName: Full=Taxane 10-beta-hydroxylase;
DE            EC=1.14.13.76;
DE   AltName: Full=5-alpha-taxadienol-10-beta-hydroxylase;
DE   AltName: Full=Cytochrome P450 725A1;
GN   Name=CYP725A1;
OS   Taxus cuspidata (Japanese yew).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Coniferopsida; Coniferales; Taxaceae; Taxus.
OX   NCBI_TaxID=99806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RX   PubMed=11171980; DOI=10.1073/pnas.98.4.1501;
RA   Schoendorf A., Rithner C.D., Williams R.M., Croteau R.B.;
RT   "Molecular cloning of a cytochrome P450 taxane 10beta-hydroxylase cDNA
RT   from Taxus and functional expression in yeast.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:1501-1506(2001).
CC   -!- FUNCTION: Involved in the transformation of a taxadienyl acetate
CC       by hydroxylation at C10 to yield taxadien-5-alpha-acetoxy-10-beta-
CC       ol.
CC   -!- CATALYTIC ACTIVITY: Taxa-4(20),11-dien-5-alpha-yl acetate + NADPH
CC       + O(2) = 10-beta-hydroxytaxa-4(20),11-dien-5-alpha-yl acetate +
CC       NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis; 10-deacetyl-2-
CC       debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol: step 2/3.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AF318211; AAK00946.1; -; mRNA.
DR   BioCyc; MetaCyc:MON-13401; -.
DR   BRENDA; 1.14.13.76; 261237.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0050597; F:taxane 10-beta-hydroxylase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase;
KW   Taxol biosynthesis.
FT   CHAIN         1    497       Taxane 10-beta-hydroxylase.
FT                                /FTId=PRO_0000052202.
FT   METAL       443    443       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   497 AA;  56691 MW;  CFE40415ABC43814 CRC64;
     MDSFIFLRSI GTKFGQLESS PAILSLTLAP ILAIILLLLF RYNHRSSVKL PPGKLGFPLI
     GETIQLLRTL RSETPQKFFD DRLKKFGPVY MTSLIGHPTV VLCGPAGNKL VLSNEDKLVE
     MEGPKSFMKL IGEDSIVAKR GEDHRILRTA LARFLGAQAL QNYLGRMSSE IGHHFNEKWK
     GKDEVKVLPL VRGLIFSIAS TLFFDVNDGH QQKQLHHLLE TILVGSLSVP LDFPGTRYRK
     GLQARLKLDE ILSSLIKRRR RDLRSGIASD DQDLLSVLLT FRDEKGNSLT DQGILDNFSA
     MFHASYDTTV APMALIFKLL YSNPEYHEKV FQEQLEIIGN KKEGEEISWK DLKSMKYTWQ
     AVQESLRMYP PVFGIFRKAI TDIHYDGYTI PKGWRVLCSP YTTHLREEYF PEPEEFRPSR
     FEDEGRHVTP YTYVPFGGGL RTCPGWEFSK IEILLFVHHF VKNFSSYIPV DPNEKVLSDP
     LPPLPANGFS IKLFPRS
//