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Protein

Taxane 10-beta-hydroxylase

Gene

CYP725A1

Organism
Taxus cuspidata (Japanese yew)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transformation of a taxadienyl acetate by hydroxylation at C10 to yield taxadien-5-alpha-acetoxy-10-beta-ol.

Catalytic activityi

Taxa-4(20),11-dien-5-alpha-yl acetate + NADPH + O2 = 10-beta-hydroxytaxa-4(20),11-dien-5-alpha-yl acetate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: taxol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 10-deacetyl-2-debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Taxadien-5-alpha-ol O-acetyltransferase (TAT)
  2. Taxane 10-beta-hydroxylase (CYP725A1)
  3. no protein annotated in this organism
This subpathway is part of the pathway taxol biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 10-deacetyl-2-debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol, the pathway taxol biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi443Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Taxol biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13401.
BRENDAi1.14.13.76. 6225.
UniPathwayiUPA00842; UER00809.

Names & Taxonomyi

Protein namesi
Recommended name:
Taxane 10-beta-hydroxylase (EC:1.14.13.76)
Alternative name(s):
5-alpha-taxadienol-10-beta-hydroxylase
Cytochrome P450 725A1
Gene namesi
Name:CYP725A1
OrganismiTaxus cuspidata (Japanese yew)
Taxonomic identifieri99806 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000522021 – 497Taxane 10-beta-hydroxylaseAdd BLAST497

Structurei

3D structure databases

ProteinModelPortaliQ9AXM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

KOiK12924.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AXM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSFIFLRSI GTKFGQLESS PAILSLTLAP ILAIILLLLF RYNHRSSVKL
60 70 80 90 100
PPGKLGFPLI GETIQLLRTL RSETPQKFFD DRLKKFGPVY MTSLIGHPTV
110 120 130 140 150
VLCGPAGNKL VLSNEDKLVE MEGPKSFMKL IGEDSIVAKR GEDHRILRTA
160 170 180 190 200
LARFLGAQAL QNYLGRMSSE IGHHFNEKWK GKDEVKVLPL VRGLIFSIAS
210 220 230 240 250
TLFFDVNDGH QQKQLHHLLE TILVGSLSVP LDFPGTRYRK GLQARLKLDE
260 270 280 290 300
ILSSLIKRRR RDLRSGIASD DQDLLSVLLT FRDEKGNSLT DQGILDNFSA
310 320 330 340 350
MFHASYDTTV APMALIFKLL YSNPEYHEKV FQEQLEIIGN KKEGEEISWK
360 370 380 390 400
DLKSMKYTWQ AVQESLRMYP PVFGIFRKAI TDIHYDGYTI PKGWRVLCSP
410 420 430 440 450
YTTHLREEYF PEPEEFRPSR FEDEGRHVTP YTYVPFGGGL RTCPGWEFSK
460 470 480 490
IEILLFVHHF VKNFSSYIPV DPNEKVLSDP LPPLPANGFS IKLFPRS
Length:497
Mass (Da):56,691
Last modified:June 1, 2001 - v1
Checksum:iCFE40415ABC43814
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF318211 mRNA. Translation: AAK00946.1.

Genome annotation databases

KEGGiag:AAK00946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF318211 mRNA. Translation: AAK00946.1.

3D structure databases

ProteinModelPortaliQ9AXM6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAK00946.

Phylogenomic databases

KOiK12924.

Enzyme and pathway databases

UniPathwayiUPA00842; UER00809.
BioCyciMetaCyc:MONOMER-13401.
BRENDAi1.14.13.76. 6225.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT10H_TAXCU
AccessioniPrimary (citable) accession number: Q9AXM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: June 1, 2001
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.