Q9AXM6 (T10H_TAXCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taxane 10-beta-hydroxylase
EC=1.14.13.76

Alternative name(s):
5-alpha-taxadienol-10-beta-hydroxylase
Cytochrome P450 725A1

Gene names

Name:

CYP725A1

Organism

Taxus cuspidata (Japanese yew)

Taxonomic identifier

99806 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Taxaceae › Taxus

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transformation of a taxadienyl acetate by hydroxylation at C10 to yield taxadien-5-alpha-acetoxy-10-beta-ol.
Catalytic activity	Taxa-4(20),11-dien-5-alpha-yl acetate + NADPH + O2 = 10-beta-hydroxytaxa-4(20),11-dien-5-alpha-yl acetate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Alkaloid biosynthesis; taxol biosynthesis; 10-deacetyl-2-debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol: step 2/3.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Taxol biosynthesis
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	paclitaxel biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro taxane 10-beta-hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Taxane 10-beta-hydroxylase		PRO_0000052202
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AXM6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CFE40415ABC43814	FASTA	497	56,691
	10 20 30 40 50 60 MDSFIFLRSI GTKFGQLESS PAILSLTLAP ILAIILLLLF RYNHRSSVKL PPGKLGFPLI 70 80 90 100 110 120 GETIQLLRTL RSETPQKFFD DRLKKFGPVY MTSLIGHPTV VLCGPAGNKL VLSNEDKLVE 130 140 150 160 170 180 MEGPKSFMKL IGEDSIVAKR GEDHRILRTA LARFLGAQAL QNYLGRMSSE IGHHFNEKWK 190 200 210 220 230 240 GKDEVKVLPL VRGLIFSIAS TLFFDVNDGH QQKQLHHLLE TILVGSLSVP LDFPGTRYRK 250 260 270 280 290 300 GLQARLKLDE ILSSLIKRRR RDLRSGIASD DQDLLSVLLT FRDEKGNSLT DQGILDNFSA 310 320 330 340 350 360 MFHASYDTTV APMALIFKLL YSNPEYHEKV FQEQLEIIGN KKEGEEISWK DLKSMKYTWQ 370 380 390 400 410 420 AVQESLRMYP PVFGIFRKAI TDIHYDGYTI PKGWRVLCSP YTTHLREEYF PEPEEFRPSR 430 440 450 460 470 480 FEDEGRHVTP YTYVPFGGGL RTCPGWEFSK IEILLFVHHF VKNFSSYIPV DPNEKVLSDP 490 LPPLPANGFS IKLFPRS

References

[1]

"Molecular cloning of a cytochrome P450 taxane 10beta-hydroxylase cDNA from Taxus and functional expression in yeast." Schoendorf A., Rithner C.D., Williams R.M., Croteau R.B. Proc. Natl. Acad. Sci. U.S.A. 98:1501-1506(2001) [PubMed: 11171980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF318211 mRNA. Translation: AAK00946.1.
3D structure databases
ProteinModelPortal	Q9AXM6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13401.
BRENDA	1.14.13.76. 6225.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T10H_TAXCU

Accession

Primary (citable) accession number: Q9AXM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	June 1, 2001
Last modified:	September 21, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program