Reviewed, UniProtKB/Swiss-Prot Q9AXH9 (KAO1_HORVU)

Names and origin

Protein names

Recommended name:
    Ent-kaurenoic acid oxidase 1
    EC=1.14.13.79
Alternative name(s):
    gpr5

Gene names

Name:	KAO1
Synonyms:	GPR5

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes three successive oxidations of ent-kaurenoic acid giving gibberellin 12 (GA12), a key step in gibberellins (GAs) biosynthesis. GAs, which are involved many processes, including stem elongation, play a central role in plant development. Ref.1
Catalytic activity	Ent-kaur-16-en-19-oate + NADPH + O2 = ent-7-alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O. Ref.1 Ent-7-alpha-hydroxykaur-16-en-19-oate + NADPH + O2 = gibberellin A(12) aldehyde + NADP+ + 2 H2O. Ref.1 Gibberellin A(12) aldehyde + NADPH + O2 = gibberellin A(12) + NADP+ + H2O. Ref.1
Cofactor	Heme group By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Ent-kaurenoic acid oxidase 1		PRO_0000052180
Regions
	Transmembrane	5 – 25	21	Potential
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity
Experimental info
	Mutagenesis	372	1	N → K in M594; induces dwarfism, due to accumulation of kaurenoic acid.

Sequences

	Sequence		Length	Mass (Da)
	Q9AXH9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 76CB0C5CC6C3641F	FASTA	499	55,876
	10 20 30 40 50 60 MGEGAWWAVA AVVAALAVVA LDAAVRAAHA WYWTASLGAG RRGRLPPGDM GWPLVGGMWA 70 80 90 100 110 120 FLRAFKSGRP DSFIDSFARR FGRAGLYRAF MFSSPTIMAT TPEACKQVLM DDDAFVTGWP 130 140 150 160 170 180 KATVALIGPK SFVNMGYDEH RRLRKLTAAP INGFDALTSY LGFIDDTVVT TLRGWSERGG 190 200 210 220 230 240 DGHFEFLTEL RRMTFRIIVQ IFMGGADERT AAELERTYTE LNYGMRAMAI DLPGFAYHKA 250 260 270 280 290 300 IRARRRLVAA LQRVLDERRA RGGKTAAGAA APVDMMDRLI AVEDEGGRRL QDDEIIDVLV 310 320 330 340 350 360 MYLNAGHESS GHITMWATVF LQENPEILAK AKAEQEAIMR SIPPGQKGLT LRDFRKMAYL 370 380 390 400 410 420 SQVVDETLRF VNISFVSFRQ ATRDVFVNGY LIPKGWKVQL WYRSVHMDPQ VYPDPKKFDP 430 440 450 460 470 480 SRWEGPPPRA GTFLPFGLGT RLCPGNDLAK LEISVFLHHF LLGYKLTRKN PNCRVRYLPH 490 PRPVDNCLAK ITRLSSSHG

References

[1]

"The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway." Helliwell C.A., Chandler P.M., Poole A., Dennis E.S., Peacock J.W. Proc. Natl. Acad. Sci. U.S.A. 98:2065-2070(2001) [PubMed: 11172076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, MUTANT M594. Strain: cv. Himalaya.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF326277 mRNA. Translation: AAK11616.1.
3D structure databases
SMR	Q9AXH9. Positions 44-494.
ModBase	Search...
Organism-specific databases
Gramene	Q9AXH9.
Enzyme and pathway databases
BRENDA	1.14.13.79. 283.
Gene expression databases
Genevestigator	Q9AXH9.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAO1_HORVU

Accession

Primary (citable) accession number: Q9AXH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)