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Q9AVR2 (Q9AVR2_SAMEB) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
rRNA N-glycosidase RuleBase RU004915
EC=3.2.2.22 RuleBase RU004915
Gene names
Name:ebu1 EMBL CAC33178.1
OrganismSambucus ebulus (Dwarf elderberry) EMBL CAC33178.1
Taxonomic identifier28503 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Dipsacales › Adoxaceae › Sambucus

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. SAAS SAAS016139 RuleBase RU004915

Sequence similarities

Belongs to the ribosome-inactivating family. RuleBase RU004915

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential EMBL CAC33178.1
Chain26 – 564539 Potential EMBL CAC33178.1
PRO_5000067897
Chain26 – 298273ebulin l A-chain EMBL CAC33178.1
PRO_5000067898
Chain299 – 564266ebulin l B-chain EMBL CAC33178.1
PRO_5000067899

Regions

Region322 – 3254Galactose binding PDB 1HWN PDB 1HWO PDB 1HWM PDB 1HWP
Region335 – 3373Galactose binding PDB 1HWN PDB 1HWO PDB 1HWM PDB 1HWP
Region342 – 3454Galactose binding PDB 1HWN PDB 1HWO PDB 1HWP
Region533 – 5375Galactose binding PDB 1HWN
Region552 – 5554Galactose binding PDB 1HWN

Sites

Binding site3251Glucose PDB 1HWO
Binding site4131Galactose PDB 1HWM
Binding site5471Galactose PDB 1HWN

Amino acid modifications

Glycosylation4841N-linked (GlcNAc...) PDB 1HWN PDB 1HWO PDB 1HWM PDB 1HWP

Sequences

Sequence LengthMass (Da)Tools
Q9AVR2 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 8261681A6DB55CB8

FASTA56462,694
        10         20         30         40         50         60 
MRVVKAAMLY LHIVVLAIYS VGIQGIDYPS VSFNLAGAKS TTYRDFLKNL RDRVATGTYE 

        70         80         90        100        110        120 
VNGLPVLRRE SEVQVKNRFV LVRLTNYNGD TVTSAVDVTN LYLVAFSANG NSYFFKDATE 

       130        140        150        160        170        180 
LQKSNLFLGT TQHTLSFTGN YDNLETAAGT RRESIELGPN PLDGAITSLW YDGGVARSLL 

       190        200        210        220        230        240 
VLIQMVPEAA RFRYIEQEVR RSLQQLTSFT PNALMLSMEN NWSSMSLEVQ LSGDNVSPFS 

       250        260        270        280        290        300 
GTVQLQNYDH TPRLVDNFEE LYKITGIAIL LFRCVATKTT HNAIRMPHVL VGEDNKFNDG 

       310        320        330        340        350        360 
ETCAIPAPFT RRIVGRDGLC VDVRNGYDTD GTPIQLWPCG TQRNQQWTFY NDKTIRSMGK 

       370        380        390        400        410        420 
CMTANGLNSG SYIMITDCST AAEDATKWEV LIDGSIINPS SGLVMTAPSG ASRTTLLLEN 

       430        440        450        460        470        480 
NIHAASQGWT VSNDVQPIAT LIVGYNEMCL QANGENNNVW MEDCDVTSVQ QQWALFDDRT 

       490        500        510        520        530        540 
IRVNNSRGLC VTSNGYVSKD LIVIRKCQGL ATQRWFFNSD GSVVNLKSTR VMDVKESDVS 

       550        560 
LQEVIIFPAT GNPNQQWRTQ VPQI

« Hide

References

[1]"Molecular cloning of ebulin l."
Girbes T., Iglesias R., Perez Y., Ferreras J.M., Citores L.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Leaves EMBL CAC33178.1.
[2]"Ebulin 1, a nontoxic novel type 2 ribosome-inactivating protein from Sambucus ebulus L. leaves."
Girbes T., Citores L., Iglesias R., Ferreras J.M., Munoz R., Rojo M.A., Arias F.J., Garcia J.R., Mendez E., Calonge M.
J. Biol. Chem. 268:18195-18199(1993) [PubMed: 8349695] [Abstract]
Cited for: PROTEIN SEQUENCE.
[3]"2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l."
Pascal J.M., Day P.J., Monzingo A.F., Ernst S.R., Robertus J.D., Iglesias R., Perez Y., Ferreras J.M., Citores L., Girbes T.
Proteins 43:319-326(2001) [PubMed: 11288182] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 26-279 AND 299-564 IN COMPLEX WITH GALACTOSE AND GLUCOSE, GLYCOSYLATION AT ASN-484.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ400822 mRNA. Translation: CAC33178.1.
PIRA47424.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HWMX-ray2.80A26-279[»]
B299-564[»]
1HWNX-ray2.80A26-279[»]
B299-564[»]
1HWOX-ray2.90A26-279[»]
B299-564[»]
1HWPX-ray3.10A26-279[»]
B299-564[»]
ProteinModelPortalQ9AVR2.
SMRQ9AVR2. Positions 26-279, 301-564.
ModBaseSearch...

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
IPR008997. Ricin_B-rel_lectin.
IPR000772. Ricin_B_lectin.
[Graphical view]
Gene3DG3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
PfamPF00652. Ricin_B_lectin. 2 hits.
PF00161. RIP. 1 hit.
[Graphical view]
PRINTSPR00396. SHIGARICIN.
SMARTSM00458. RICIN. 2 hits.
[Graphical view]
SUPFAMSSF56371. Ribosome_inactivat_prot. 1 hit.
SSF50370. RicinB_like. 2 hits.
PROSITEPS50231. RICIN_B_LECTIN. 2 hits.
PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ9AVR2_SAMEB
AccessionPrimary (citable) accession number: Q9AVR2
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info