Unreviewed, UniProtKB/TrEMBL Q9AVR2 (Q9AVR2_9DIPS)

Names and origin

Protein names

Submitted name:
    Ribosome-inactivating protein EMBL CAC33178.1
    EC=3.2.2.22

Gene names

Name:

ebu1 EMBL CAC33178.1

Organism

Sambucus ebulus EMBL CAC33178.1

Taxonomic identifier

28503 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Dipsacales › Adoxaceae › Sambucus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Spearmint SPM016138

Ontologies

Keywords
Biological process	Plant defense RuleBase RU000426V2
Domain	Signal
Molecular function	Glycosidase Hydrolase Protein synthesis inhibitor Spearmint SPM016138 Toxin Spearmint SPM016138
Gene Ontology (GO)
Biological process	negative regulation of translation Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential EMBL CAC33178.1
	Chain	26 – 564	539	Potential EMBL CAC33178.1		PRO_5000067897
	Chain	26 – 298	273	ebulin l A-chain EMBL CAC33178.1		PRO_5000067898
	Chain	299 – 564	266	ebulin l B-chain EMBL CAC33178.1		PRO_5000067899

Sequences

	Sequence		Length	Mass (Da)
	Q9AVR2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8261681A6DB55CB8	FASTA	564	62,694
	10 20 30 40 50 60 MRVVKAAMLY LHIVVLAIYS VGIQGIDYPS VSFNLAGAKS TTYRDFLKNL RDRVATGTYE 70 80 90 100 110 120 VNGLPVLRRE SEVQVKNRFV LVRLTNYNGD TVTSAVDVTN LYLVAFSANG NSYFFKDATE 130 140 150 160 170 180 LQKSNLFLGT TQHTLSFTGN YDNLETAAGT RRESIELGPN PLDGAITSLW YDGGVARSLL 190 200 210 220 230 240 VLIQMVPEAA RFRYIEQEVR RSLQQLTSFT PNALMLSMEN NWSSMSLEVQ LSGDNVSPFS 250 260 270 280 290 300 GTVQLQNYDH TPRLVDNFEE LYKITGIAIL LFRCVATKTT HNAIRMPHVL VGEDNKFNDG 310 320 330 340 350 360 ETCAIPAPFT RRIVGRDGLC VDVRNGYDTD GTPIQLWPCG TQRNQQWTFY NDKTIRSMGK 370 380 390 400 410 420 CMTANGLNSG SYIMITDCST AAEDATKWEV LIDGSIINPS SGLVMTAPSG ASRTTLLLEN 430 440 450 460 470 480 NIHAASQGWT VSNDVQPIAT LIVGYNEMCL QANGENNNVW MEDCDVTSVQ QQWALFDDRT 490 500 510 520 530 540 IRVNNSRGLC VTSNGYVSKD LIVIRKCQGL ATQRWFFNSD GSVVNLKSTR VMDVKESDVS 550 560 LQEVIIFPAT GNPNQQWRTQ VPQI

References

[1]	"Molecular cloning of ebulin l." Girbes T., Iglesias R., Perez Y., Ferreras J.M., Citores L. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Leaves EMBL CAC33178.1.
[2]	"Ebulin 1, a nontoxic novel type 2 ribosome-inactivating protein from Sambucus ebulus L. leaves." Girbes T., Citores L., Iglesias R., Ferreras J.M., Munoz R., Rojo M.A., Arias F.J., Garcia J.R., Mendez E., Calonge M. J. Biol. Chem. 268:18195-18199(1993) [PubMed: 8349695] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l." Pascal J.M., Day P.J., Monzingo A.F., Ernst S.R., Robertus J.D., Iglesias R., Perez Y., Ferreras J.M., Citores L., Girbes T. Proteins 43:319-326(2001) [PubMed: 11288182] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 299-564.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ400822 mRNA. Translation: CAC33178.1.

PIR

A47424.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HWM	X-ray	2.80	A	26-279	[»]
			B	299-564	[»]
1HWN	X-ray	2.80	A	26-279	[»]
			B	299-564	[»]
1HWO	X-ray	2.90	A	26-279	[»]
			B	299-564	[»]
1HWP	X-ray	3.10	A	26-279	[»]
			B	299-564	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM13. Carbohydrate-Binding Module Family 13.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
IPR000772. Ricin_B_lectin.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00652. Ricin_B_lectin. 2 hits.
PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

SMART

SM00458. RICIN. 2 hits.
[Graphical view]

PROSITE

PS50231. RICIN_B_LECTIN. 2 hits.
PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9AVR2_9DIPS

Accession

Primary (citable) accession number: Q9AVR2

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)