Reviewed, UniProtKB/Swiss-Prot Q9AV97 (KDSA_ARATH)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS
      Short name=AtkdsA

Gene names

Name:	KDSA
Ordered Locus Names:	At1g79500
ORF Names:	T8K14.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rhamnogalacturonan II biosynthetic process Traceable author statement. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9AV97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	2-dehydro-3-deoxyphosphooctonate aldolase		PRO_0000187179
Experimental info
	Sequence conflict	2	1	A → T in AAM47465. Ref.3
	Sequence conflict	140	1	F → L in BAB41014. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 2C79A24D66D4FACB	FASTA	290	31,657
	10 20 30 40 50 60 MAATSLLYNQ LKAAEPFFLL AGPNVIESEE HILRMAKHIK DISTKVGLPL VFKSSFDKAN 70 80 90 100 110 120 RTSSKSFRGP GMAEGLKILE KVKVAYDLPI VTDVHESSQC EAVGKVADII QIPAFLCRQT 130 140 150 160 170 180 DLLVAAAQTG KIINIKKGQF CAPSVMENSA EKIRLAGNPN VMVCERGTMF GYNDLIVDPR 190 200 210 220 230 240 NFEWMREANC PVVADITHSL QQPAGKKLDG GGVASGGLRE LIPCIARTAV AVGVDGIFME 250 260 270 280 290 VHDDPLSAPV DGPTQWPLRH LEELLEELIA IARVTKGKQR LQIDLTPYRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate synthase: cDNA cloning and expression analyses." Matsuura K., Miyagawa I., Kobayashi M., Ohta D., Matoh T. J. Exp. Bot. 54:1785-1787(2003) [PubMed: 12754267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB059683 mRNA. Translation: BAB41014.1. AC007202 Genomic DNA. Translation: AAD30227.1. AF372947 mRNA. Translation: AAK50087.1. AY113162 mRNA. Translation: AAM47465.1.
IPI	IPI00542948.
PIR	C96826.
RefSeq	NP_001031300.1. NP_178068.1. NP_849906.1. NP_974176.1.
UniGene	At.20828
3D structure databases
HSSP	HSSP built from PDB template 1FY6 based on UniProtKB O66496.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9AV97.
Proteomic databases
PRIDE	Q9AV97.
Genome annotation databases
GeneID	844288.
GenomeReviews	Gene locus AT1G79500 in contig CT485782_GR.
KEGG	ath:AT1G79500.
NMPDR	fig|3702.1.peg.7513.
Organism-specific databases
TAIR	At1g79500.
Phylogenomic databases
OMA	DPRNFEW.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G79500-MON.
BRENDA	2.5.1.55. 302.
Gene expression databases
ArrayExpress	Q9AV97.
Genevestigator	Q9AV97.
GermOnline	AT1G79500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_ARATH

Accession

Primary (citable) accession number: Q9AV97
Secondary accession number(s): Q8L890, Q9SAJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	May 10, 2002
Last modified:	November 3, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)