Q9AV71 (CESA7_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulose synthase A catalytic subunit 7 [UDP-forming]
EC=2.4.1.12

Alternative name(s):
OsCesA7

Gene names

Name:	CESA7
Ordered Locus Names:	Os10g0467800, LOC_Os10g32980
ORF Names:	OSJNBa0006L06.10

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1063 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity. Involved in the secondary cell wall formation. Ref.5
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Disruption phenotype	Plants develop a brittle culm (bc) phenotype with a reduction of up to 75% percent of cellulose content in culm. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1063	1063	Cellulose synthase A catalytic subunit 7 [UDP-forming]		PRO_0000319367
Regions
	Topological domain	1 – 213	213	Cytoplasmic Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Topological domain	235 – 237	3	Extracellular Potential
	Transmembrane	238 – 258	21	Helical; Potential
	Topological domain	259 – 845	587	Cytoplasmic Potential
	Transmembrane	846 – 866	21	Helical; Potential
	Topological domain	867 – 871	5	Extracellular Potential
	Transmembrane	872 – 892	21	Helical; Potential
	Topological domain	893 – 907	15	Cytoplasmic Potential
	Transmembrane	908 – 928	21	Helical; Potential
	Topological domain	929 – 959	31	Extracellular Potential
	Transmembrane	960 – 980	21	Helical; Potential
	Topological domain	981 – 991	11	Cytoplasmic Potential
	Transmembrane	992 – 1012	21	Helical; Potential
	Topological domain	1013 – 1021	9	Extracellular Potential
	Transmembrane	1022 – 1042	21	Helical; Potential
	Topological domain	1043 – 1063	21	Cytoplasmic Potential
	Zinc finger	18 – 64	47	RING-type; degenerate
	Coiled coil	387 – 414	28	Potential
Sites
	Active site	333	1	Potential
	Active site	762	1	Potential
	Metal binding	18	1	Zinc 1 By similarity
	Metal binding	21	1	Zinc 1 By similarity
	Metal binding	37	1	Zinc 2 By similarity
	Metal binding	40	1	Zinc 2 By similarity
	Metal binding	45	1	Zinc 1 By similarity
	Metal binding	48	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	63	1	Zinc 2 By similarity
	Binding site	499	1	Substrate Potential
	Binding site	501	1	Substrate Potential
Amino acid modifications
	Glycosylation	935	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9AV71 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BE02386EFAECDE4E	FASTA	1,063	119,788
	10 20 30 40 50 60 MDTASVTGGE HKGKEKTCRV CGEEVAARED GKPFVACAEC GFPVCKPCYE YERSEGTQCC 70 80 90 100 110 120 PQCNTRYKRH KGCPRVEGDE DDGGDMDDFE EEFQIKSPTK QKPPHEPVNF DVYSENGEQP 130 140 150 160 170 180 AQKWRPGGPA LSSFTGSVAG KDLEQEREME GGMEWKDRID KWKTKQEKRG KLNRDDSDDD 190 200 210 220 230 240 DDKNDDEYML LAEARQPLWR KVPIPSSKIN PYRIVIVLRL VVLCFFLKFR ITTPAMDAVP 250 260 270 280 290 300 LWLASVICEL WFALSWILDQ LPKWSPVTRE TYLDRLALRY ERDGEPCRLA PIDFFVSTVD 310 320 330 340 350 360 PLKEPPIITA NTVLSILAVD YPVDRVSCYV SDDGASMLLF DTLSETAEFA RRWVPFCKKF 370 380 390 400 410 420 TIEPRAPEFY FSQKIDYLKD KVQPTFVKER RAMKREYEEF KVRINALVAK AQKKPEEGWV 430 440 450 460 470 480 MQDGTPWPGN NTRDHPGMIQ VYLGSQGALD VEGSELPRLV YVSREKRPGY NHHKKAGAMN 490 500 510 520 530 540 SLVRVSAVLT NAPFILNLDC DHYVNNSKAV REAMCFLMDK QLGKKLCYVQ FPQRFDGIDR 550 560 570 580 590 600 HDRYANRNTV FFDINMKGLD GIQGPVYVGT GTVFNRQALY GYDPPRPEKR PKMTCDCWPS 610 620 630 640 650 660 WCCCCCCFGG GKRGKSHKNK KGGGGGEGGG LDEPRRGLLG FYKKRSKKDK LGGGAASLAG 670 680 690 700 710 720 GKKGYRKHQR GFELEEIEEG LEGYDELERS SLMSQKSFEK RFGQSPVFIA STLVEDGGLP 730 740 750 760 770 780 QGAAADPAAL IKEAIHVISC GYEEKTEWGK EIGWIYGSVT EDILTGFKMH CRGWKSVYCT 790 800 810 820 830 840 PARAAFKGSA PINLSDRLHQ VLRWALGSVE IFMSRHCPLW YAYGGRLKWL ERFAYTNTIV 850 860 870 880 890 900 YPFTSIPLLA YCTIPAVCLL TGKFIIPTLN NLASIWFIAL FLSIIATGVL ELRWSGVSIE 910 920 930 940 950 960 DWWRNEQFWV IGGVSAHLFA VFQGLLKVLG GVDTNFTVTS KAAADETDAF GELYLFKWTT 970 980 990 1000 1010 1020 LLVPPTTLII INMVGIVAGV SDAVNNGYGS WGPLFGKLFF SFWVILHLYP FLKGLMGRQN 1030 1040 1050 1060 RTPTIVVLWS ILLASIFSLV WVRIDPFIPK PKGPVLKPCG VSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall." Tanaka K., Murata K., Yamazaki M., Onosato K., Miyao A., Hirochika H. Plant Physiol. 133:73-83(2003) [PubMed: 12970476] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC022457 Genomic DNA. Translation: AAK27814.1. DP000086 Genomic DNA. Translation: AAP54202.1. AP008216 Genomic DNA. Translation: BAF26725.1. AK072259 mRNA. No translation available.
RefSeq	NP_001064811.1. NM_001071346.1.
UniGene	Os.3206.
3D structure databases
ProteinModelPortal	Q9AV71.
SMR	Q9AV71. Positions 18-76.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9AV71.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os10g32980.1; LOC_Os10g32980.1; LOC_Os10g32980.
GeneID	4348853.
KEGG	osa:4348853.
NMPDR	fig|39947.1.peg.13425.
Organism-specific databases
Gramene	Q9AV71.
Phylogenomic databases
GeneTree	EPGT00070000027964.
HOGENOM	HBG744549.
OMA	LLAYCTI.
PhylomeDB	Q9AV71.
ProtClustDB	PLN02915.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KO	K10999.
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA7_ORYSJ

Accession

Primary (citable) accession number: Q9AV71
Secondary accession number(s): Q7XDK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program