Q9AUR8 (COPA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coatomer subunit alpha-1

Alternative name(s):
Alpha-coat protein 1
Short name=Alpha-COP 1

Gene names

Ordered Locus Names:	Os03g0711400, LOC_Os03g50340
ORF Names:	OsJ_011822, OSJNBb0033N16.10

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	1218 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins By similarity.
Subunit structure	Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits By similarity.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity.
Sequence similarities	Contains 9 WD repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	COPI vesicle coat Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1218	1218	Coatomer subunit alpha-1		PRO_0000285601
Regions
	Repeat	7 – 48	42	WD 1
	Repeat	49 – 88	40	WD 2
	Repeat	91 – 132	42	WD 3
	Repeat	133 – 172	40	WD 4
	Repeat	202 – 241	40	WD 5
	Repeat	246 – 285	40	WD 6
	Repeat	288 – 326	39	WD 7
	Repeat	363 – 404	42	WD 8
	Repeat	450 – 489	40	WD 9

Sequences

	Sequence		Length	Mass (Da)
	Q9AUR8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D8B06A8E0F14EB9D	FASTA	1,218	136,381
	10 20 30 40 50 60 MLTKFETKSN RVKGLSFHPR RPWILASLHS GVIQMWDYRM GTLLDRFDEH DGPVRGVHFH 70 80 90 100 110 120 ATQPLFVSGG DDYKIKVWNY KTHRCLFTLH GHLDYIRTVQ FHHEYPWIVS ASDDQTIRIW 130 140 150 160 170 180 NWQSRTCVAV LTGHNHYVMC ASFHPKEDLV VSASLDQTVR VWDIGALRKK TVSPADDILR 190 200 210 220 230 240 LTQMNTDLFG GVDAVVKYVL EGHDRGVNWA SFHPTLPLIV SGADDRQVKL WRMNDTKAWE 250 260 270 280 290 300 VDTLRGHMNN VSCVMFHAKQ DIIVSNSEDK SIRIWDATKR TGIQTFRREH DRFWILSAHP 310 320 330 340 350 360 EMNLLAAGHD SGMIVFKLER ERPAFSVSGD TVFYVKDRFL RFFEFTTQKE VQLAPIRRPG 370 380 390 400 410 420 SVSLNQSPKT LSYSPTENAV LICSDVDGGS YELYIVPKDS AGRADYLQDA KKGAGGSAVF 430 440 450 460 470 480 VARNRFAVLE KSSNQVLVKN LKNEIVKKSP LPIATDAIYY AGTGNLLCKA EDRVTIFDLQ 490 500 510 520 530 540 QRLILGELQA PSVKYVVWSS DMESVALLSK HAVVIANKKL VHRCTLHETI RVKSGAWDEN 550 560 570 580 590 600 GVFIYTTLNH IKYCLPNGDS GIIKTLDVPI YITRVIGNNI FCLDRDGKNK LVTVDASEYI 610 620 630 640 650 660 FKLALLRKRY DHVMSMIKNS QLCGQAVISY LQQKGFPEVA LHFVKDEKTR FNLALESGNI 670 680 690 700 710 720 QIAVASAKEI DDKDHWYRLG IEALRQGNVG IVEYAYQRTK NFERLAFLYL ITGYMDKVGF 730 740 750 760 770 780 MCKIAGQNNN LMGQFHNALY LGDALKRVEI LENAGQLPLA YITATTHGLT EIADRLAAEL 790 800 810 820 830 840 GENIPSLPEG KARSLLIPPA PLTASGDWPL LRVMHGIFEG GLDATGKAEL EEDDEAAGAD 850 860 870 880 890 900 WGDEDLDMVD ASEAMANGGD GFDAEEGEAN EEDGEEGGWD LEDLELPPEA ETPKNAGNAR 910 920 930 940 950 960 SAVFVAPPPG MPVSLIWTQK SSLAGEHAAA GNFDTAMRLL SRQLGIKNFA PLKPLFVDLH 970 980 990 1000 1010 1020 MGSHSYLRAL ATAPIIPIAV EKGWSESASP NVRGPPALVF TFPQMEDRLK AAYKATTDGK 1030 1040 1050 1060 1070 1080 FPEALRQFLS ILHTIPLIVV DSRREVDEVK ELIEIVREYV LGLRMELKRK ELRDDVNRQQ 1090 1100 1110 1120 1130 1140 ELAAYFTNCK LQRVHMRLVL GSAMGLCYKQ KNFATAEHFA RMLLENNPNE AQARRARQVQ 1150 1160 1170 1180 1190 1200 QQCSGKKDSS ELNYDYRNPF VVCGATYVPI YRGQKDVSCP YCGSRFVPSI EGQLCTICEL 1210 AVVGADASGL LCSPTQSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC082645 Genomic DNA. Translation: AAK18834.1. DP000009 Genomic DNA. Translation: ABF98513.1. AP008209 Genomic DNA. Translation: BAF12970.1. CM000140 Genomic DNA. Translation: EAZ28339.1. AK100286 mRNA. No translation available.
RefSeq	NP_001051056.1. NM_001057591.1.
UniGene	Os.37960.
3D structure databases
HSSP	HSSP built from PDB template 1VYH based on UniProtKB P63005.
ProteinModelPortal	Q9AUR8.
ModBase	Search...
Proteomic databases
PaxDb	Q9AUR8.
PRIDE	Q9AUR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g50340.1; LOC_Os03g50340.1; LOC_Os03g50340.
GeneID	4333885.
KEGG	osa:4333885.
Organism-specific databases
Gramene	Q9AUR8.
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000195913.
KO	K05236.
OMA	TIFCLER.
ProtClustDB	CLSN2919403.
Gene expression databases
ArrayExpress	Q9AUR8.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR016391. Coatomer_asu. IPR010714. Coatomer_asu_C. IPR006692. Coatomer_WD-assoc_reg. IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF04053. Coatomer_WDAD. 1 hit. PF06957. COPI_C. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PIRSF	PIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COPA1_ORYSJ

Accession

Primary (citable) accession number: Q9AUR8
Secondary accession number(s): Q10E15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program