Reviewed, UniProtKB/Swiss-Prot Q9AUE0 (CALS1_ARATH)

Names and origin

Protein names

Recommended name:
    Callose synthase 1
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 6

Gene names

Name:	CALS1
Synonyms:	GSL6
Ordered Locus Names:	At1g05570
ORF Names:	F3F20.1, T25N20.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in callose synthesis at the forming cell plate during cytokinesis. Not required for callose formation after wounding or pathogen attack. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.6
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Enzyme regulation	May be regulated by ROP1 through the interaction with UGT1.
Subunit structure	Interacts with UGT1 and phragmoplastin. May form a functional complex with UGT1, ROP1 and phragmoplastin. Ref.1 Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Localized in the forming cell plate during cytokinesis. Ref.1 Ref.5
Sequence similarities	Belongs to the glycosyltransferase 48 family.
Sequence caution	The sequence AAD30609.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF79729.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	1,3-beta-glucan synthase complex Inferred from electronic annotation. Source: InterPro cell plate Ref.1 Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1950	1950	Callose synthase 1		PRO_0000334573
Regions
	Topological domain	1 – 481	481	Cytoplasmic Potential
	Transmembrane	482 – 502	21	Potential
	Topological domain	503 – 505	3	Extracellular Potential
	Transmembrane	506 – 526	21	Potential
	Topological domain	527 – 552	26	Cytoplasmic Potential
	Transmembrane	553 – 573	21	Potential
	Topological domain	574 – 599	26	Extracellular Potential
	Transmembrane	600 – 620	21	Potential
	Topological domain	621 – 655	35	Cytoplasmic Potential
	Transmembrane	656 – 676	21	Potential
	Topological domain	677 – 712	36	Extracellular Potential
	Transmembrane	713 – 733	21	Potential
	Topological domain	734 – 1511	778	Cytoplasmic Potential
	Transmembrane	1512 – 1532	21	Potential
	Topological domain	1533 – 1560	28	Extracellular Potential
	Transmembrane	1561 – 1581	21	Potential
	Topological domain	1582 – 1591	10	Cytoplasmic Potential
	Transmembrane	1592 – 1612	21	Potential
	Topological domain	1613 – 1655	43	Extracellular Potential
	Transmembrane	1656 – 1676	21	Potential
	Topological domain	1677 – 1682	6	Cytoplasmic Potential
	Transmembrane	1683 – 1703	21	Potential
	Topological domain	1704 – 1755	52	Extracellular Potential
	Transmembrane	1756 – 1776	21	Potential
	Topological domain	1777 – 1787	11	Cytoplasmic Potential
	Transmembrane	1788 – 1808	21	Potential
	Topological domain	1809 – 1828	20	Extracellular Potential
	Transmembrane	1829 – 1849	21	Potential
	Topological domain	1850 – 1851	2	Cytoplasmic Potential
	Transmembrane	1852 – 1872	21	Potential
	Topological domain	1873 – 1894	22	Extracellular Potential
	Transmembrane	1895 – 1915	21	Potential
	Topological domain	1916 – 1950	35	Cytoplasmic Potential
	Compositional bias	7 – 12	6	Poly-Pro
Amino acid modifications
	Glycosylation	577	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	88	1	T → A in AAK37413. Ref.1
	Sequence conflict	126	1	Q → R in AAK37413. Ref.1
	Sequence conflict	560	1	A → S in AAK37413. Ref.1
	Sequence conflict	725	1	L → P in AAK37413. Ref.1
	Sequence conflict	1268 – 1270	3	KGT → EGA in AAK37413. Ref.1
	Sequence conflict	1396	1	V → A in BAF00852. Ref.3
	Sequence conflict	1653	1	R → H in BAF00852. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9AUE0-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 25EA79B202BA7443	FASTA	1,950	225,761
	10 20 30 40 50 60 MAQRREPDPP PPQRRILRTQ TVGSLGEAML DSEVVPSSLV EIAPILRVAN EVEASNPRVA 70 80 90 100 110 120 YLCRFYAFEK AHRLDPTSSG RGVRQFKTAL LQRLERENET TLAGRQKSDA REMQSFYQHY 130 140 150 160 170 180 YKKYIQALLN AADKADRAQL TKAYQTAAVL FEVLKAVNQT EDVEVADEIL ETHNKVEEKT 190 200 210 220 230 240 QIYVPYNILP LDPDSQNQAI MRLPEIQAAV AALRNTRGLP WTAGHKKKLD EDILDWLQSM 250 260 270 280 290 300 FGFQKDNVLN QREHLILLLA NVHIRQFPKP DQQPKLDDRA LTIVMKKLFR NYKKWCKYLG 310 320 330 340 350 360 RKSSLWLPTI QQEVQQRKLL YMGLYLLIWG EAANLRFMPE CLCYIYHHMA FELYGMLAGS 370 380 390 400 410 420 VSPMTGEHVK PAYGGEDEAF LQKVVTPIYQ TISKEAKRSR GGKSKHSVWR NYDDLNEYFW 430 440 450 460 470 480 SIRCFRLGWP MRADADFFCQ TAEELRLERS EIKSNSGDRW MGKVNFVEIR SFWHIFRSFD 490 500 510 520 530 540 RLWSFYILCL QAMIVIAWNG SGELSAIFQG DVFLKVLSVF ITAAILKLAQ AVLDIALSWK 550 560 570 580 590 600 ARHSMSLYVK LRYVMKVGAA AVWVVVMAVT YAYSWKNASG FSQTIKNWFG GHSHNSPSLF 610 620 630 640 650 660 IVAILIYLSP NMLSALLFLF PFIRRYLERS DYKIMMLMMW WSQPRLYIGR GMHESALSLF 670 680 690 700 710 720 KYTMFWIVLL ISKLAFSYYA EIKPLVGPTK DIMRIHISVY SWHEFFPHAK NNLGVVIALW 730 740 750 760 770 780 SPVILVYFMD TQIWYAIVST LVGGLNGAFR RLGEIRTLGM LRSRFQSIPG AFNDCLVPQD 790 800 810 820 830 840 NSDDTKKKRF RATFSRKFDQ LPSSKDKEAA RFAQMWNKII SSFREEDLIS DREMELLLVP 850 860 870 880 890 900 YWSDPDLDLI RWPPFLLASK IPIALDMAKD SNGKDRELKK RLAVDSYMTC AVRECYASFK 910 920 930 940 950 960 NLINYLVVGE REGQVINDIF SKIDEHIEKE TLITELNLSA LPDLYGQFVR LIEYLLENRE 970 980 990 1000 1010 1020 EDKDQIVIVL LNMLELVTRD IMEEEVPSLL ETAHNGSYVK YDVMTPLHQQ RKYFSQLRFP 1030 1040 1050 1060 1070 1080 VYSQTEAWKE KIKRLHLLLT VKESAMDVPS NLEARRRLTF FSNSLFMDMP PAPKIRNMLS 1090 1100 1110 1120 1130 1140 FSVLTPYFSE DVLFSIFGLE QQNEDGVSIL FYLQKIFPDE WTNFLERVKC GNEEELRARE 1150 1160 1170 1180 1190 1200 DLEEELRLWA SYRGQTLTKT VRGMMYYRKA LELQAFLDMA KDEELLKGYK ALELTSEEAS 1210 1220 1230 1240 1250 1260 KSGGSLWAQC QALADMKFTF VVSCQQYSIH KRSGDQRAKD ILRLMTTYPS IRVAYIDEVE 1270 1280 1290 1300 1310 1320 QTHKESYKGT EEKIYYSALV KAAPQTKPMD SSESVQTLDQ LIYRIKLPGP AILGEGKPEN 1330 1340 1350 1360 1370 1380 QNHAIIFTRG EGLQTIDMNQ DNYMEEAFKM RNLLQEFLEK HGGVRCPTIL GLREHIFTGS 1390 1400 1410 1420 1430 1440 VSSLAWFMSN QENSFVTIGQ RVLASPLKVR FHYGHPDIFD RLFHLTRGGI CKASKVINLS 1450 1460 1470 1480 1490 1500 EDIFAGFNST LREGNVTHHE YIQVGKGRDV GLNQISMFEA KIANGNGEQT LSRDLYRLGH 1510 1520 1530 1540 1550 1560 RFDFFRMLSC YFTTIGFYFS TMLTVLTVYV FLYGRLYLVL SGLEEGLSSQ RAFRNNKPLE 1570 1580 1590 1600 1610 1620 AALASQSFVQ IGFLMALPMM MEIGLERGFH NALIEFVLMQ LQLASVFFTF QLGTKTHYYG 1630 1640 1650 1660 1670 1680 RTLFHGGAEY RGTGRGFVVF HAKFAENYRF YSRSHFVKGI ELMILLLVYQ IFGQSYRGVV 1690 1700 1710 1720 1730 1740 TYILITVSIW FMVVTWLFAP FLFNPSGFEW QKIVDDWTDW NKWIYNRGGI GVPPEKSWES 1750 1760 1770 1780 1790 1800 WWEKELEHLR HSGVRGITLE IFLALRFFIF QYGLVYHLST FKGKNQSFWV YGASWFVILF 1810 1820 1830 1840 1850 1860 ILLIVKGLGV GRRRFSTNFQ LLFRIIKGLV FLTFVAILIT FLALPLITIK DLFICMLAFM 1870 1880 1890 1900 1910 1920 PTGWGMLLIA QACKPLIQQL GIWSSVRTLA RGYEIVMGLL LFTPVAFLAW FPFVSEFQTR 1930 1940 1950 MLFNQAFSRG LQISRILGGQ RKDRSSKNKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE, INTERACTION WITH UGT1 AND PHRAGMOPLASTIN. Strain: cv. Columbia. Tissue: Shoot meristem.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1202-1950. Strain: cv. Columbia.
[4]	"A novel UDP-glucose transferase is part of the callose synthase complex and interacts with phragmoplastin at the forming cell plate." Hong Z., Zhang Z., Olson J.M., Verma D.P.S. Plant Cell 13:769-779(2001) [PubMed: 11283335] [Abstract] Cited for: SUBUNIT, INTERACTION WITH UGT1.
[5]	"Plant callose synthase complexes." Verma D.P.S., Hong Z. Plant Mol. Biol. 47:693-701(2001) [PubMed: 11785931] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation." Jacobs A.K., Lipka V., Burton R.A., Panstruga R., Strizhov N., Schulze-Lefert P., Fincher G.B. Plant Cell 15:2503-2513(2003) [PubMed: 14555698] [Abstract] Cited for: FUNCTION.
[7]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF237733 mRNA. Translation: AAK37413.1. AC005106 Genomic DNA. Translation: AAF79729.1. Sequence problems. AC007153 Genomic DNA. Translation: AAD30609.1. Sequence problems. AK228963 mRNA. Translation: BAF00852.1.
IPI	IPI00544652.
PIR	E86189.
RefSeq	NP_563743.2.
UniGene	At.15333
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9AUE0. 2 interactions.
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Proteomic databases
PRIDE	Q9AUE0.
Genome annotation databases
GeneID	837059.
GenomeReviews	Gene locus AT1G05570 in contig CT485782_GR.
KEGG	ath:AT1G05570.
Organism-specific databases
TAIR	At1g05570.
Family and domain databases
InterPro	IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF02364. Glucan_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALS1_ARATH

Accession

Primary (citable) accession number: Q9AUE0
Secondary accession number(s): Q0WPU7, Q9LR43, Q9SYJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)