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Protein

Callose synthase 1

Gene

CALS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in callose synthesis at the forming cell plate during cytokinesis. Not required for callose formation after wounding or pathogen attack. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.1 Publication

Catalytic activityi

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Enzyme regulationi

May be regulated by ROP1 through the interaction with UGT1.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G05570-MONOMER.
ARA:GQT-2827-MONOMER.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
Callose synthase 1 (EC:2.4.1.34)
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 6
Gene namesi
Name:CALS1
Synonyms:GSL6
Ordered Locus Names:At1g05570
ORF Names:F3F20.1, T25N20.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G05570.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 481481CytoplasmicSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 5053ExtracellularSequence analysis
Transmembranei506 – 52621HelicalSequence analysisAdd
BLAST
Topological domaini527 – 55226CytoplasmicSequence analysisAdd
BLAST
Transmembranei553 – 57321HelicalSequence analysisAdd
BLAST
Topological domaini574 – 59926ExtracellularSequence analysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence analysisAdd
BLAST
Topological domaini621 – 65535CytoplasmicSequence analysisAdd
BLAST
Transmembranei656 – 67621HelicalSequence analysisAdd
BLAST
Topological domaini677 – 71236ExtracellularSequence analysisAdd
BLAST
Transmembranei713 – 73321HelicalSequence analysisAdd
BLAST
Topological domaini734 – 1511778CytoplasmicSequence analysisAdd
BLAST
Transmembranei1512 – 153221HelicalSequence analysisAdd
BLAST
Topological domaini1533 – 156028ExtracellularSequence analysisAdd
BLAST
Transmembranei1561 – 158121HelicalSequence analysisAdd
BLAST
Topological domaini1582 – 159110CytoplasmicSequence analysis
Transmembranei1592 – 161221HelicalSequence analysisAdd
BLAST
Topological domaini1613 – 165543ExtracellularSequence analysisAdd
BLAST
Transmembranei1656 – 167621HelicalSequence analysisAdd
BLAST
Topological domaini1677 – 16826CytoplasmicSequence analysis
Transmembranei1683 – 170321HelicalSequence analysisAdd
BLAST
Topological domaini1704 – 175552ExtracellularSequence analysisAdd
BLAST
Transmembranei1756 – 177621HelicalSequence analysisAdd
BLAST
Topological domaini1777 – 178711CytoplasmicSequence analysisAdd
BLAST
Transmembranei1788 – 180821HelicalSequence analysisAdd
BLAST
Topological domaini1809 – 182820ExtracellularSequence analysisAdd
BLAST
Transmembranei1829 – 184921HelicalSequence analysisAdd
BLAST
Topological domaini1850 – 18512CytoplasmicSequence analysis
Transmembranei1852 – 187221HelicalSequence analysisAdd
BLAST
Topological domaini1873 – 189422ExtracellularSequence analysisAdd
BLAST
Transmembranei1895 – 191521HelicalSequence analysisAdd
BLAST
Topological domaini1916 – 195035CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • 1,3-beta-D-glucan synthase complex Source: InterPro
  • cell plate Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19501950Callose synthase 1PRO_0000334573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi577 – 5771N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9AUE0.
PRIDEiQ9AUE0.

PTM databases

iPTMnetiQ9AUE0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9AUE0. baseline and differential.
GenevisibleiQ9AUE0. AT.

Interactioni

Subunit structurei

Interacts with UGT1 and phragmoplastin. May form a functional complex with UGT1, ROP1 and phragmoplastin.2 Publications

Protein-protein interaction databases

BioGridi22301. 1 interaction.
IntActiQ9AUE0. 2 interactions.
STRINGi3702.AT1G05570.1.

Structurei

3D structure databases

ProteinModelPortaliQ9AUE0.
SMRiQ9AUE0. Positions 34-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 126Poly-Pro

Sequence similaritiesi

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ9AUE0.
KOiK11000.
OMAiRCPTILG.
PhylomeDBiQ9AUE0.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026953. CALS.
IPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PANTHERiPTHR12741:SF9. PTHR12741:SF9. 1 hit.
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 2 hits.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9AUE0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQRREPDPP PPQRRILRTQ TVGSLGEAML DSEVVPSSLV EIAPILRVAN
60 70 80 90 100
EVEASNPRVA YLCRFYAFEK AHRLDPTSSG RGVRQFKTAL LQRLERENET
110 120 130 140 150
TLAGRQKSDA REMQSFYQHY YKKYIQALLN AADKADRAQL TKAYQTAAVL
160 170 180 190 200
FEVLKAVNQT EDVEVADEIL ETHNKVEEKT QIYVPYNILP LDPDSQNQAI
210 220 230 240 250
MRLPEIQAAV AALRNTRGLP WTAGHKKKLD EDILDWLQSM FGFQKDNVLN
260 270 280 290 300
QREHLILLLA NVHIRQFPKP DQQPKLDDRA LTIVMKKLFR NYKKWCKYLG
310 320 330 340 350
RKSSLWLPTI QQEVQQRKLL YMGLYLLIWG EAANLRFMPE CLCYIYHHMA
360 370 380 390 400
FELYGMLAGS VSPMTGEHVK PAYGGEDEAF LQKVVTPIYQ TISKEAKRSR
410 420 430 440 450
GGKSKHSVWR NYDDLNEYFW SIRCFRLGWP MRADADFFCQ TAEELRLERS
460 470 480 490 500
EIKSNSGDRW MGKVNFVEIR SFWHIFRSFD RLWSFYILCL QAMIVIAWNG
510 520 530 540 550
SGELSAIFQG DVFLKVLSVF ITAAILKLAQ AVLDIALSWK ARHSMSLYVK
560 570 580 590 600
LRYVMKVGAA AVWVVVMAVT YAYSWKNASG FSQTIKNWFG GHSHNSPSLF
610 620 630 640 650
IVAILIYLSP NMLSALLFLF PFIRRYLERS DYKIMMLMMW WSQPRLYIGR
660 670 680 690 700
GMHESALSLF KYTMFWIVLL ISKLAFSYYA EIKPLVGPTK DIMRIHISVY
710 720 730 740 750
SWHEFFPHAK NNLGVVIALW SPVILVYFMD TQIWYAIVST LVGGLNGAFR
760 770 780 790 800
RLGEIRTLGM LRSRFQSIPG AFNDCLVPQD NSDDTKKKRF RATFSRKFDQ
810 820 830 840 850
LPSSKDKEAA RFAQMWNKII SSFREEDLIS DREMELLLVP YWSDPDLDLI
860 870 880 890 900
RWPPFLLASK IPIALDMAKD SNGKDRELKK RLAVDSYMTC AVRECYASFK
910 920 930 940 950
NLINYLVVGE REGQVINDIF SKIDEHIEKE TLITELNLSA LPDLYGQFVR
960 970 980 990 1000
LIEYLLENRE EDKDQIVIVL LNMLELVTRD IMEEEVPSLL ETAHNGSYVK
1010 1020 1030 1040 1050
YDVMTPLHQQ RKYFSQLRFP VYSQTEAWKE KIKRLHLLLT VKESAMDVPS
1060 1070 1080 1090 1100
NLEARRRLTF FSNSLFMDMP PAPKIRNMLS FSVLTPYFSE DVLFSIFGLE
1110 1120 1130 1140 1150
QQNEDGVSIL FYLQKIFPDE WTNFLERVKC GNEEELRARE DLEEELRLWA
1160 1170 1180 1190 1200
SYRGQTLTKT VRGMMYYRKA LELQAFLDMA KDEELLKGYK ALELTSEEAS
1210 1220 1230 1240 1250
KSGGSLWAQC QALADMKFTF VVSCQQYSIH KRSGDQRAKD ILRLMTTYPS
1260 1270 1280 1290 1300
IRVAYIDEVE QTHKESYKGT EEKIYYSALV KAAPQTKPMD SSESVQTLDQ
1310 1320 1330 1340 1350
LIYRIKLPGP AILGEGKPEN QNHAIIFTRG EGLQTIDMNQ DNYMEEAFKM
1360 1370 1380 1390 1400
RNLLQEFLEK HGGVRCPTIL GLREHIFTGS VSSLAWFMSN QENSFVTIGQ
1410 1420 1430 1440 1450
RVLASPLKVR FHYGHPDIFD RLFHLTRGGI CKASKVINLS EDIFAGFNST
1460 1470 1480 1490 1500
LREGNVTHHE YIQVGKGRDV GLNQISMFEA KIANGNGEQT LSRDLYRLGH
1510 1520 1530 1540 1550
RFDFFRMLSC YFTTIGFYFS TMLTVLTVYV FLYGRLYLVL SGLEEGLSSQ
1560 1570 1580 1590 1600
RAFRNNKPLE AALASQSFVQ IGFLMALPMM MEIGLERGFH NALIEFVLMQ
1610 1620 1630 1640 1650
LQLASVFFTF QLGTKTHYYG RTLFHGGAEY RGTGRGFVVF HAKFAENYRF
1660 1670 1680 1690 1700
YSRSHFVKGI ELMILLLVYQ IFGQSYRGVV TYILITVSIW FMVVTWLFAP
1710 1720 1730 1740 1750
FLFNPSGFEW QKIVDDWTDW NKWIYNRGGI GVPPEKSWES WWEKELEHLR
1760 1770 1780 1790 1800
HSGVRGITLE IFLALRFFIF QYGLVYHLST FKGKNQSFWV YGASWFVILF
1810 1820 1830 1840 1850
ILLIVKGLGV GRRRFSTNFQ LLFRIIKGLV FLTFVAILIT FLALPLITIK
1860 1870 1880 1890 1900
DLFICMLAFM PTGWGMLLIA QACKPLIQQL GIWSSVRTLA RGYEIVMGLL
1910 1920 1930 1940 1950
LFTPVAFLAW FPFVSEFQTR MLFNQAFSRG LQISRILGGQ RKDRSSKNKE
Length:1,950
Mass (Da):225,761
Last modified:May 20, 2008 - v2
Checksum:i25EA79B202BA7443
GO

Sequence cautioni

The sequence AAD30609.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAF79729.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881T → A in AAK37413 (PubMed:11283334).Curated
Sequence conflicti126 – 1261Q → R in AAK37413 (PubMed:11283334).Curated
Sequence conflicti560 – 5601A → S in AAK37413 (PubMed:11283334).Curated
Sequence conflicti725 – 7251L → P in AAK37413 (PubMed:11283334).Curated
Sequence conflicti1268 – 12703KGT → EGA in AAK37413 (PubMed:11283334).Curated
Sequence conflicti1396 – 13961V → A in BAF00852 (Ref. 4) Curated
Sequence conflicti1653 – 16531R → H in BAF00852 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237733 mRNA. Translation: AAK37413.1.
AC005106 Genomic DNA. Translation: AAF79729.1. Sequence problems.
AC007153 Genomic DNA. Translation: AAD30609.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27855.1.
AK228963 mRNA. Translation: BAF00852.1.
PIRiE86189.
RefSeqiNP_563743.2. NM_100436.4. [Q9AUE0-1]
UniGeneiAt.15333.

Genome annotation databases

EnsemblPlantsiAT1G05570.1; AT1G05570.1; AT1G05570. [Q9AUE0-1]
GeneIDi837059.
KEGGiath:AT1G05570.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237733 mRNA. Translation: AAK37413.1.
AC005106 Genomic DNA. Translation: AAF79729.1. Sequence problems.
AC007153 Genomic DNA. Translation: AAD30609.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27855.1.
AK228963 mRNA. Translation: BAF00852.1.
PIRiE86189.
RefSeqiNP_563743.2. NM_100436.4. [Q9AUE0-1]
UniGeneiAt.15333.

3D structure databases

ProteinModelPortaliQ9AUE0.
SMRiQ9AUE0. Positions 34-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22301. 1 interaction.
IntActiQ9AUE0. 2 interactions.
STRINGi3702.AT1G05570.1.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

PTM databases

iPTMnetiQ9AUE0.

Proteomic databases

PaxDbiQ9AUE0.
PRIDEiQ9AUE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G05570.1; AT1G05570.1; AT1G05570. [Q9AUE0-1]
GeneIDi837059.
KEGGiath:AT1G05570.

Organism-specific databases

TAIRiAT1G05570.

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ9AUE0.
KOiK11000.
OMAiRCPTILG.
PhylomeDBiQ9AUE0.

Enzyme and pathway databases

BioCyciARA:AT1G05570-MONOMER.
ARA:GQT-2827-MONOMER.

Miscellaneous databases

PROiQ9AUE0.

Gene expression databases

ExpressionAtlasiQ9AUE0. baseline and differential.
GenevisibleiQ9AUE0. AT.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026953. CALS.
IPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PANTHERiPTHR12741:SF9. PTHR12741:SF9. 1 hit.
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 2 hits.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cell plate-specific callose synthase and its interaction with phragmoplastin."
    Hong Z., Delauney A.J., Verma D.P.S.
    Plant Cell 13:755-768(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE, INTERACTION WITH UGT1 AND PHRAGMOPLASTIN.
    Strain: cv. Columbia.
    Tissue: Shoot meristem.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1202-1950.
    Strain: cv. Columbia.
  5. "A novel UDP-glucose transferase is part of the callose synthase complex and interacts with phragmoplastin at the forming cell plate."
    Hong Z., Zhang Z., Olson J.M., Verma D.P.S.
    Plant Cell 13:769-779(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH UGT1.
  6. Cited for: SUBCELLULAR LOCATION.
  7. "An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation."
    Jacobs A.K., Lipka V., Burton R.A., Panstruga R., Strizhov N., Schulze-Lefert P., Fincher G.B.
    Plant Cell 15:2503-2513(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
    Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
    Plant Mol. Biol. 58:333-349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiCALS1_ARATH
AccessioniPrimary (citable) accession number: Q9AUE0
Secondary accession number(s): Q0WPU7, Q9LR43, Q9SYJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 8, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.