Q9ASW1 (B3GTG_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 16
EC=2.4.1.-

Gene names

Name:	B3GALT16
Ordered Locus Names:	At3g06440
ORF Names:	F24P17.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in juvenile leaves, stems, cauline leaves and siliques. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence AAF08572.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ASW1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ASW1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     552-559: LFKLEDVA → VSFVVSSP
     560-619: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Probable beta-1,3-galactosyltransferase 16		PRO_0000359426
Regions
	Transmembrane	21 – 37	17	Helical; Signal-anchor for type II membrane protein; Potential
	Domain	166 – 344	179	Galectin
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	568	1	N-linked (GlcNAc...) Potential
	Glycosylation	613	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	552 – 559	8	LFKLEDVA → VSFVVSSP in isoform 2.		VSP_036149
	Alternative sequence	560 – 619	60	Missing in isoform 2.		VSP_036150
Experimental info
	Sequence conflict	2	1	K → Q in AAQ77230. Ref.1
	Sequence conflict	130	1	N → S in AAQ77231. Ref.1
	Sequence conflict	134	1	R → G in AAQ77230. Ref.1
	Sequence conflict	207	1	R → C in AAQ77230. Ref.1
	Sequence conflict	207	1	R → C in AAQ77231. Ref.1
	Sequence conflict	285	1	F → S in AAQ77231. Ref.1
	Sequence conflict	311	1	R → W in AAQ77230. Ref.1
	Sequence conflict	342	1	L → I in AAQ77230. Ref.1
	Sequence conflict	368	1	G → E in AAQ77230. Ref.1
	Sequence conflict	428	1	K → T in AAQ77230. Ref.1
	Sequence conflict	428	1	K → T in AAQ77231. Ref.1
	Sequence conflict	439	1	V → C in AAQ77231. Ref.1
	Sequence conflict	467 – 470	4	KTDD → MSGC in AAQ77231. Ref.1
	Sequence conflict	590	1	N → K in AAQ77231. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 755F1DFC9ECFDBD8	FASTA	619	70,635
	10 20 30 40 50 60 MKQFMSVVRF KFGFTSVRMR DWSVGVSIMV LTLIFIIRYE QSDHTHTVDD SSIEGESVHE 70 80 90 100 110 120 PAKKPHFMTL EDLDYLFSNK SFFGEEEVSN GMLVWSRMRP FLERPDALPE TAQGIEEATL 130 140 150 160 170 180 AMKGLVLEIN REKRAYSSGM VSKEIRRICP DFVTAFDKDL SGLSHVLLEL PCGLIEDSSI 190 200 210 220 230 240 TLVGIPDEHS SSFQIQLVGS GLSGETRRPI ILRYNVNFSK PSIVQNTWTE KLGWGNEERC 250 260 270 280 290 300 QYHGSLKNHL VDELPLCNKQ TGRIISEKSS NDDATMELSL SNANFPFLKG SPFTAALWFG 310 320 330 340 350 360 LEGFHMTING RHETSFAYRE KLEPWLVSAV KVSGGLKILS VLATRLPIPD DHASLIIEEK 370 380 390 400 410 420 LKAPSLSGTR IELLVGVFST GNNFKRRMAL RRSWMQYEAV RSGKVAVRFL IGLHTNEKVN 430 440 450 460 470 480 LEMWRESKAY GDIQFMPFVD YYGLLSLKTV ALCILGTKVI PAKYIMKTDD DAFVRIDELL 490 500 510 520 530 540 SSLEERPSSA LLYGLISFDS SPDREQGSKW FIPKEEWPLD SYPPWAHGPG YIISHDIAKF 550 560 570 580 590 600 VVKGHRQRDL GLFKLEDVAM GIWIQQFNQT IKRVKYINDK RFHNSDCKSN YILVHYQTPR 610 LILCLWEKLQ KENQSICCE
	Isoform 2 [UniParc]. Checksum: 43D8BB478152040A	FASTA	559	63,180
	10 20 30 40 50 60 MKQFMSVVRF KFGFTSVRMR DWSVGVSIMV LTLIFIIRYE QSDHTHTVDD SSIEGESVHE 70 80 90 100 110 120 PAKKPHFMTL EDLDYLFSNK SFFGEEEVSN GMLVWSRMRP FLERPDALPE TAQGIEEATL 130 140 150 160 170 180 AMKGLVLEIN REKRAYSSGM VSKEIRRICP DFVTAFDKDL SGLSHVLLEL PCGLIEDSSI 190 200 210 220 230 240 TLVGIPDEHS SSFQIQLVGS GLSGETRRPI ILRYNVNFSK PSIVQNTWTE KLGWGNEERC 250 260 270 280 290 300 QYHGSLKNHL VDELPLCNKQ TGRIISEKSS NDDATMELSL SNANFPFLKG SPFTAALWFG 310 320 330 340 350 360 LEGFHMTING RHETSFAYRE KLEPWLVSAV KVSGGLKILS VLATRLPIPD DHASLIIEEK 370 380 390 400 410 420 LKAPSLSGTR IELLVGVFST GNNFKRRMAL RRSWMQYEAV RSGKVAVRFL IGLHTNEKVN 430 440 450 460 470 480 LEMWRESKAY GDIQFMPFVD YYGLLSLKTV ALCILGTKVI PAKYIMKTDD DAFVRIDELL 490 500 510 520 530 540 SSLEERPSSA LLYGLISFDS SPDREQGSKW FIPKEEWPLD SYPPWAHGPG YIISHDIAKF 550 VVKGHRQRDL GVSFVVSSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana putative beta 1,3 galactosyltransferase." Kiefer-Meyer M.-C., Faye L., Gomord V. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: cv. Wassilewskija.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY305276 mRNA. Translation: AAQ77230.1. AY305277 mRNA. Translation: AAQ77231.1. AC011623 Genomic DNA. Translation: AAF08572.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE74393.1. CP002686 Genomic DNA. Translation: AEE74394.1. AF361640 mRNA. Translation: AAK32808.1. BT002218 mRNA. Translation: AAN72229.1.
IPI	IPI00519740. IPI00846216.
RefSeq	NP_001078115.1. NM_001084646.1. NP_566284.1. NM_111519.2.
UniGene	At.28708.
3D structure databases
ProteinModelPortal	Q9ASW1.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q9ASW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G06440.1; AT3G06440.1; AT3G06440.
GeneID	819820.
GenomeReviews	Gene locus AT3G06440 in contig BA000014_GR.
KEGG	ath:AT3G06440.
NMPDR	fig|3702.1.peg.12647.
Organism-specific databases
TAIR	At3g06440.
Phylogenomic databases
eggNOG	KOG2287.
GeneTree	EPGT00070000028922.
HOGENOM	HBG318248.
InParanoid	Q9ASW1.
OMA	GRHETSF.
PhylomeDB	Q9ASW1.
ProtClustDB	CLSN2688114.
Gene expression databases
Genevestigator	Q9ASW1.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 2 hits.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 1 hit. SM00276. GLECT. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTG_ARATH

Accession

Primary (citable) accession number: Q9ASW1
Secondary accession number(s): Q4VWQ4, Q4VWQ5, Q9SQU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program