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Protein

Diacylglycerol O-acyltransferase 2

Gene

DGAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA (PubMed:23770095, PubMed:24663078). Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA (PubMed:23770095).2 Publications

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

  • diacylglycerol O-acyltransferase activity Source: TAIR

GO - Biological processi

  • glycerol metabolic process Source: UniProtKB-KW
  • lipid particle organization Source: TAIR
  • neutral lipid biosynthetic process Source: TAIR
  • triglyceride biosynthetic process Source: TAIR

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G51520-MONOMER.
ReactomeiR-ATH-1482883. Acyl chain remodeling of DAG and TAG.
R-ATH-2142753. Arachidonic acid metabolism.
R-ATH-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-ATH-75109. Triglyceride Biosynthesis.
R-ATH-8848584. Wax biosynthesis.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2Curated (EC:2.3.1.201 Publication)
Short name:
AtDGAT21 Publication
Gene namesi
Name:DGAT21 Publication
Ordered Locus Names:At3g51520
ORF Names:F26O13.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2081865. AT3G51520.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: TAIR

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Disruption phenotypei

No decrease in oil content.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986141 – 314Diacylglycerol O-acyltransferase 2Add BLAST314

Proteomic databases

PaxDbiQ9ASU1.

Expressioni

Tissue specificityi

Ubiquitous. Lower levels in seeds than in other tissues. Expressed in embryo and root meristematic cells (PubMed:23042274).2 Publications

Gene expression databases

ExpressionAtlasiQ9ASU1. baseline and differential.
GenevisibleiQ9ASU1. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G51520.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ASU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
HOGENOMiHOG000239410.
InParanoidiQ9ASU1.
KOiK14457.
OMAiVQETFHM.
OrthoDBiEOG09360FKQ.
PhylomeDBiQ9ASU1.

Family and domain databases

InterProiView protein in InterPro
IPR007130. DAGAT.
PfamiView protein in Pfam
PF03982. DAGAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ASU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGSREFRAE EHSNQFHSII AMAIWLGAIH FNVALVLCSL IFLPPSLSLM
60 70 80 90 100
VLGLLSLFIF IPIDHRSKYG RKLARYICKH ACNYFPVSLY VEDYEAFQPN
110 120 130 140 150
RAYVFGYEPH SVLPIGVVAL CDLTGFMPIP NIKVLASSAI FYTPFLRHIW
160 170 180 190 200
TWLGLTAASR KNFTSLLDSG YSCVLVPGGV QETFHMQHDA ENVFLSRRRG
210 220 230 240 250
FVRIAMEQGS PLVPVFCFGQ ARVYKWWKPD CDLYLKLSRA IRFTPICFWG
260 270 280 290 300
VFGSPLPCRQ PMHVVVGKPI EVTKTLKPTD EEIAKFHGQY VEALRDLFER
310
HKSRVGYDLE LKIL
Length:314
Mass (Da):35,854
Last modified:June 1, 2001 - v1
Checksum:i28DC9E0B50FF5949
GO

Sequence cautioni

The sequence CAB63016 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133452 Genomic DNA. Translation: CAB63016.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78802.1.
AF361832 mRNA. Translation: AAK32844.1.
AY078045 mRNA. Translation: AAL77746.1.
AY087571 mRNA. Translation: AAM65113.1.
PIRiT45783.
RefSeqiNP_566952.1. NM_115011.3.
UniGeneiAt.28717.

Genome annotation databases

EnsemblPlantsiAT3G51520.1; AT3G51520.1; AT3G51520.
GeneIDi824315.
GrameneiAT3G51520.1; AT3G51520.1; AT3G51520.
KEGGiath:AT3G51520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133452 Genomic DNA. Translation: CAB63016.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78802.1.
AF361832 mRNA. Translation: AAK32844.1.
AY078045 mRNA. Translation: AAL77746.1.
AY087571 mRNA. Translation: AAM65113.1.
PIRiT45783.
RefSeqiNP_566952.1. NM_115011.3.
UniGeneiAt.28717.

3D structure databases

ProteinModelPortaliQ9ASU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G51520.1.

Proteomic databases

PaxDbiQ9ASU1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G51520.1; AT3G51520.1; AT3G51520.
GeneIDi824315.
GrameneiAT3G51520.1; AT3G51520.1; AT3G51520.
KEGGiath:AT3G51520.

Organism-specific databases

AraportiAT3G51520.
TAIRilocus:2081865. AT3G51520.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
HOGENOMiHOG000239410.
InParanoidiQ9ASU1.
KOiK14457.
OMAiVQETFHM.
OrthoDBiEOG09360FKQ.
PhylomeDBiQ9ASU1.

Enzyme and pathway databases

UniPathwayiUPA00282.
BioCyciARA:AT3G51520-MONOMER.
ReactomeiR-ATH-1482883. Acyl chain remodeling of DAG and TAG.
R-ATH-2142753. Arachidonic acid metabolism.
R-ATH-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-ATH-75109. Triglyceride Biosynthesis.
R-ATH-8848584. Wax biosynthesis.

Miscellaneous databases

PROiPR:Q9ASU1.

Gene expression databases

ExpressionAtlasiQ9ASU1. baseline and differential.
GenevisibleiQ9ASU1. AT.

Family and domain databases

InterProiView protein in InterPro
IPR007130. DAGAT.
PfamiView protein in Pfam
PF03982. DAGAT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDGAT2_ARATH
AccessioniPrimary (citable) accession number: Q9ASU1
Secondary accession number(s): Q9SCZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: April 12, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.