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Protein

Elongation factor 2

Gene

LOS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity). Involved in cold responses leading to freezing tolerance via the induction of cold-responsive genes (PubMed:9401119, PubMed:12032361).By similarity2 Publications

Caution

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 34GTPBy similarity6
Nucleotide bindingi104 – 108GTPSequence analysis5
Nucleotide bindingi158 – 161GTPBy similarity4

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • mRNA binding Source: TAIR
  • translation elongation factor activity Source: UniProtKB-KW

GO - Biological processi

  • cold acclimation Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-156902 Peptide chain elongation
R-ATH-5358493 Synthesis of diphthamide-EEF2
R-ATH-6798695 Neutrophil degranulation
R-ATH-8876725 Protein methylation
UniPathwayiUPA00345

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2Curated
Short name:
EF-2Curated
Alternative name(s):
Protein LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 11 Publication
Gene namesi
Name:LOS11 Publication
Ordered Locus Names:At1g56070/At1g56075Imported
ORF Names:T6H22.13Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G56070
TAIRilocus:2205235 AT1G56070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Blocks specifically low temperature-induced transcription of cold-responsive genes such as RD29A (PubMed:9401119, PubMed:12032361). Reduced capacity to develop freezing tolerance but does not impair the vernalization response. Defective in protein synthesis in the cold (PubMed:12032361).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004355641 – 843Elongation factor 2Add BLAST843

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei837PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ASR1
PRIDEiQ9ASR1
ProMEXiQ9ASR1

PTM databases

iPTMnetiQ9ASR1
SwissPalmiQ9ASR1

Expressioni

Tissue specificityi

Expressed in root, stem, leaves, flowers and siliques.1 Publication

Inductioni

Induced by cold.1 Publication

Gene expression databases

ExpressionAtlasiQ9ASR1 baseline and differential

Interactioni

Subunit structurei

May interact with glutaredoxins (Grxs).1 Publication

Protein-protein interaction databases

IntActiQ9ASR1 2 interactors.
MINTiQ9ASR1
STRINGi3702.AT1G56070.1

Structurei

3D structure databases

ProteinModelPortaliQ9ASR1
SMRiQ9ASR1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 344tr-type GPROSITE-ProRule annotationAdd BLAST328

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469 Eukaryota
COG0480 LUCA
HOGENOMiHOG000231589
KOiK03234
OMAiEHLISGM
OrthoDBiEOG093601SM
PhylomeDBiQ9ASR1

Family and domain databases

Gene3Di3.30.230.102 hits
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ASR1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKFTADELR RIMDYKHNIR NMSVIAHVDH GKSTLTDSLV AAAGIIAQEV
60 70 80 90 100
AGDVRMTDTR ADEAERGITI KSTGISLYYE MTDESLKSFT GARDGNEYLI
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR ITDGALVVVD CIEGVCVQTE TVLRQALGER
160 170 180 190 200
IRPVLTVNKM DRCFLELQVD GEEAYQTFSR VIENANVIMA TYEDPLLGDV
210 220 230 240 250
QVYPEKGTVA FSAGLHGWAF TLTNFAKMYA SKFGVVESKM MERLWGENFF
260 270 280 290 300
DPATRKWSGK NTGSPTCKRG FVQFCYEPIK QIIATCMNDQ KDKLWPMLAK
310 320 330 340 350
LGVSMKNDEK ELMGKPLMKR VMQTWLPAST ALLEMMIFHL PSPHTAQRYR
360 370 380 390 400
VENLYEGPLD DQYANAIRNC DPNGPLMLYV SKMIPASDKG RFFAFGRVFA
410 420 430 440 450
GKVSTGMKVR IMGPNYIPGE KKDLYTKSVQ RTVIWMGKRQ ETVEDVPCGN
460 470 480 490 500
TVAMVGLDQF ITKNATLTNE KEVDAHPIRA MKFSVSPVVR VAVQCKVASD
510 520 530 540 550
LPKLVEGLKR LAKSDPMVVC TMEESGEHIV AGAGELHLEI CLKDLQDDFM
560 570 580 590 600
GGAEIIKSDP VVSFRETVCD RSTRTVMSKS PNKHNRLYME ARPMEEGLAE
610 620 630 640 650
AIDDGRIGPR DDPKIRSKIL AEEFGWDKDL AKKIWAFGPE TTGPNMVVDM
660 670 680 690 700
CKGVQYLNEI KDSVVAGFQW ASKEGPLAEE NMRGICFEVC DVVLHSDAIH
710 720 730 740 750
RGGGQVIPTA RRVIYASQIT AKPRLLEPVY MVEIQAPEGA LGGIYSVLNQ
760 770 780 790 800
KRGHVFEEMQ RPGTPLYNIK AYLPVVESFG FSSQLRAATS GQAFPQCVFD
810 820 830 840
HWEMMSSDPL EPGTQASVLV ADIRKRKGLK EAMTPLSEFE DKL
Length:843
Mass (Da):93,891
Last modified:June 1, 2001 - v1
Checksum:i861551554192B2AB
GO
Isoform 2 (identifier: Q9ASR1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-664: Missing.
     818-828: VLVADIRKRKG → FWWLISGRGRD
     829-843: Missing.

Note: No experimental confirmation available.Imported
Show »
Length:213
Mass (Da):23,622
Checksum:i427DB185DA7FB535
GO

Sequence cautioni

The sequence AAF02837 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD94254 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAD94268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05811350 – 664Missing in isoform 2. Add BLAST615
Alternative sequenceiVSP_058114818 – 828VLVADIRKRKG → FWWLISGRGRD in isoform 2. Add BLAST11
Alternative sequenceiVSP_058115829 – 843Missing in isoform 2. Add BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA Translation: AAF02837.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33338.1
CP002684 Genomic DNA Translation: ANM58611.1
CP002684 Genomic DNA Translation: ANM58612.1
AF367331 mRNA Translation: AAK32918.1
AY035011 mRNA Translation: AAK59516.2
AY054461 mRNA Translation: AAK96653.1
BT000661 mRNA Translation: AAN31808.1
BT000722 mRNA Translation: AAN31864.1
BT000786 mRNA Translation: AAN31925.1
BT002714 mRNA Translation: AAO11630.1
BT006187 mRNA Translation: AAP04170.1
AK319019 mRNA Translation: BAH57134.1
AK220706 mRNA Translation: BAD93810.1
AK221896 mRNA Translation: BAD94254.1 Different initiation.
AK221900 mRNA Translation: BAD94268.1 Different initiation.
PIRiA96602
RefSeqiNP_001321033.1, NM_001333759.1 [Q9ASR1-1]
NP_001321034.1, NM_001333760.1 [Q9ASR1-1]
NP_849818.1, NM_179487.2 [Q9ASR1-1]
UniGeneiAt.47544

Genome annotation databases

EnsemblPlantsiAT1G56070.1; AT1G56070.1; AT1G56070 [Q9ASR1-1]
AT1G56070.2; AT1G56070.2; AT1G56070 [Q9ASR1-1]
AT1G56070.3; AT1G56070.3; AT1G56070 [Q9ASR1-1]
GeneIDi842058
GrameneiAT1G56070.1; AT1G56070.1; AT1G56070 [Q9ASR1-1]
AT1G56070.2; AT1G56070.2; AT1G56070 [Q9ASR1-1]
AT1G56070.3; AT1G56070.3; AT1G56070 [Q9ASR1-1]
KEGGiath:AT1G56070

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEF2_ARATH
AccessioniPrimary (citable) accession number: Q9ASR1
Secondary accession number(s): C0Z355
, Q56WX9, Q56WY3, Q570K2, Q84R07, Q8H145, Q94CA4, Q9SGT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome