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Q9ASR0 (TBB3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tubulin beta-3 chain
Gene names
Name:TUBB3
Synonyms:TUB3
Ordered Locus Names:At5g62700
ORF Names:MRG21.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit structure

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Subcellular location

Cytoplasmcytoskeleton.

Miscellaneous

There are nine genes coding for beta-tubulin. The sequences coded by genes 2 and 3 are identical.

Sequence similarities

Belongs to the tubulin family.

Sequence caution

The sequence AAK32919.1 differs from that shown. Reason: Frameshift at position 337.

The sequence AAM16247.1 differs from that shown. Reason: Frameshift at position 337.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Tubulin beta-3 chain
PRO_0000419523

Regions

Nucleotide binding140 – 1467GTP Potential

Sequences

Sequence LengthMass (Da)Tools
Q9ASR0 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: DF4C996D75A9A219

FASTA45050,734
        10         20         30         40         50         60 
MREILHIQGG QCGNQIGAKF WEVVCAEHGI DPTGRYTGDS DLQLERINVY YNEASCGRFV 

        70         80         90        100        110        120 
PRAVLMDLEP GTMDSLRSGP YGQTFRPDNF VFGQSGAGNN WAKGHYTEGA ELIDSVLDVV 

       130        140        150        160        170        180 
RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMLTFSVF PSPKVSDTVV 

       190        200        210        220        230        240 
EPYNATLSVH QLVENADECM VLDNEALYDI CFRTLKLTTP SFGDLNHLIS ATMSGVTCCL 

       250        260        270        280        290        300 
RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRSLTVPELT QQMWDSKNMM 

       310        320        330        340        350        360 
CAADPRHGRY LTASAMFRGK MSTKEVDEQM LNVQNKNSSY FVEWIPNNVK STVCDIPPTG 

       370        380        390        400        410        420 
LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS 

       430        440        450 
EYQQYQDATA DEEGDYEDEE EGEYQQEEEY 

« Hide

References

« Hide 'large scale' references
[1]"The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes."
Snustad D.P., Haas N.A., Kopczak S.D., Silflow C.D.
Plant Cell 4:549-556(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84701 Genomic DNA. Translation: AAA32882.1.
AB009053, AB020751 Genomic DNA. Translation: BAB10838.1.
CP002688 Genomic DNA. Translation: AED97644.1.
AF367332 mRNA. Translation: AAK32919.1. Frameshift.
AY060550 mRNA. Translation: AAL31181.1.
AY093986 mRNA. Translation: AAM16247.1. Frameshift.
PIRJQ1587.
RefSeqNP_568959.1. NM_125664.3.
NP_568960.1. NM_125665.3.
UniGeneAt.24202.
At.49208.

3D structure databases

ProteinModelPortalQ9ASR0.
SMRQ9ASR0. Positions 1-431.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9ASR0. 1 interaction.

Proteomic databases

PRIDEQ9ASR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G62690.1; AT5G62690.1; AT5G62690.
AT5G62700.1; AT5G62700.1; AT5G62700.
GeneID836390.
836391.
KEGGath:AT5G62690.
ath:AT5G62700.

Organism-specific databases

TAIRAT5G62700.

Phylogenomic databases

eggNOGCOG5023.
HOGENOMHOG000165710.
KOK07375.
PhylomeDBQ9ASR0.

Family and domain databases

Gene3D1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERPTHR11588. PTHR11588. 1 hit.
PfamPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTBB3_ARATH
AccessionPrimary (citable) accession number: Q9ASR0
Secondary accession number(s): P29512
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names