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Q9ASR0

- TBB3_ARATH

UniProt

Q9ASR0 - TBB3_ARATH

Protein

Tubulin beta-3 chain

Gene

TUBB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi140 – 1467GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. structural constituent of cytoskeleton Source: InterPro

    GO - Biological processi

    1. microtubule-based process Source: InterPro
    2. protein polymerization Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_216620. Post-chaperonin tubulin folding pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tubulin beta-3 chain
    Gene namesi
    Name:TUBB3
    Synonyms:TUB3
    Ordered Locus Names:At5g62700
    ORF Names:MRG21.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G62700.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 450450Tubulin beta-3 chainPRO_0000419523Add
    BLAST

    Proteomic databases

    PRIDEiQ9ASR0.

    Interactioni

    Subunit structurei

    Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

    Protein-protein interaction databases

    IntActiQ9ASR0. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ASR0.
    SMRiQ9ASR0. Positions 1-431.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tubulin family.Curated

    Phylogenomic databases

    eggNOGiCOG5023.
    HOGENOMiHOG000165710.
    KOiK07375.
    PhylomeDBiQ9ASR0.

    Family and domain databases

    Gene3Di1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProiIPR013838. Beta-tubulin_BS.
    IPR002453. Beta_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view]
    PANTHERiPTHR11588. PTHR11588. 1 hit.
    PfamiPF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view]
    PRINTSiPR01163. BETATUBULIN.
    PR01161. TUBULIN.
    SMARTiSM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEiPS00227. TUBULIN. 1 hit.
    PS00228. TUBULIN_B_AUTOREG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ASR0-1 [UniParc]FASTAAdd to Basket

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    MREILHIQGG QCGNQIGAKF WEVVCAEHGI DPTGRYTGDS DLQLERINVY    50
    YNEASCGRFV PRAVLMDLEP GTMDSLRSGP YGQTFRPDNF VFGQSGAGNN 100
    WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL 150
    LISKIREEYP DRMMLTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM 200
    VLDNEALYDI CFRTLKLTTP SFGDLNHLIS ATMSGVTCCL RFPGQLNSDL 250
    RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRSLTVPELT QQMWDSKNMM 300
    CAADPRHGRY LTASAMFRGK MSTKEVDEQM LNVQNKNSSY FVEWIPNNVK 350
    STVCDIPPTG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG 400
    EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEEGDYEDEE EGEYQQEEEY 450
    Length:450
    Mass (Da):50,734
    Last modified:October 3, 2012 - v2
    Checksum:iDF4C996D75A9A219
    GO

    Sequence cautioni

    The sequence AAK32919.1 differs from that shown. Reason: Frameshift at position 337.
    The sequence AAM16247.1 differs from that shown. Reason: Frameshift at position 337.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84701 Genomic DNA. Translation: AAA32882.1.
    AB009053, AB020751 Genomic DNA. Translation: BAB10838.1.
    CP002688 Genomic DNA. Translation: AED97644.1.
    AF367332 mRNA. Translation: AAK32919.1. Frameshift.
    AY060550 mRNA. Translation: AAL31181.1.
    AY093986 mRNA. Translation: AAM16247.1. Frameshift.
    PIRiJQ1587.
    RefSeqiNP_568959.1. NM_125664.3.
    NP_568960.1. NM_125665.3.
    UniGeneiAt.24202.
    At.49208.

    Genome annotation databases

    EnsemblPlantsiAT5G62690.1; AT5G62690.1; AT5G62690.
    AT5G62700.1; AT5G62700.1; AT5G62700.
    GeneIDi836390.
    836391.
    KEGGiath:AT5G62690.
    ath:AT5G62700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84701 Genomic DNA. Translation: AAA32882.1 .
    AB009053 , AB020751 Genomic DNA. Translation: BAB10838.1 .
    CP002688 Genomic DNA. Translation: AED97644.1 .
    AF367332 mRNA. Translation: AAK32919.1 . Frameshift.
    AY060550 mRNA. Translation: AAL31181.1 .
    AY093986 mRNA. Translation: AAM16247.1 . Frameshift.
    PIRi JQ1587.
    RefSeqi NP_568959.1. NM_125664.3.
    NP_568960.1. NM_125665.3.
    UniGenei At.24202.
    At.49208.

    3D structure databases

    ProteinModelPortali Q9ASR0.
    SMRi Q9ASR0. Positions 1-431.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9ASR0. 1 interaction.

    Proteomic databases

    PRIDEi Q9ASR0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G62690.1 ; AT5G62690.1 ; AT5G62690 .
    AT5G62700.1 ; AT5G62700.1 ; AT5G62700 .
    GeneIDi 836390.
    836391.
    KEGGi ath:AT5G62690.
    ath:AT5G62700.

    Organism-specific databases

    TAIRi AT5G62700.

    Phylogenomic databases

    eggNOGi COG5023.
    HOGENOMi HOG000165710.
    KOi K07375.
    PhylomeDBi Q9ASR0.

    Enzyme and pathway databases

    Reactomei REACT_216620. Post-chaperonin tubulin folding pathway.

    Family and domain databases

    Gene3Di 1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProi IPR013838. Beta-tubulin_BS.
    IPR002453. Beta_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view ]
    PANTHERi PTHR11588. PTHR11588. 1 hit.
    Pfami PF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01163. BETATUBULIN.
    PR01161. TUBULIN.
    SMARTi SM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEi PS00227. TUBULIN. 1 hit.
    PS00228. TUBULIN_B_AUTOREG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes."
      Snustad D.P., Haas N.A., Kopczak S.D., Silflow C.D.
      Plant Cell 4:549-556(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiTBB3_ARATH
    AccessioniPrimary (citable) accession number: Q9ASR0
    Secondary accession number(s): P29512
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are nine genes coding for beta-tubulin. The sequences coded by genes 2 and 3 are identical.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3