Q9ASI1 (NIP41_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin NIP4-1

Alternative name(s):
NOD26-like intrinsic protein 4-1
OsNIP4;1

Gene names

Name:	NIP4-1
Ordered Locus Names:	Os01g0112400, LOC_Os01g02190
ORF Names:	OSJNBb0032H19.9, P0439B06.35

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in leaves and at lower levels in roots. Ref.5
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC 1.A.8.12) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Aquaporin NIP4-1		PRO_0000286038
Regions
	Transmembrane	59 – 79	21	Helical; Name=1; Potential
	Transmembrane	86 – 106	21	Helical; Name=2; Potential
	Transmembrane	133 – 153	21	Helical; Name=3; Potential
	Transmembrane	173 – 193	21	Helical; Name=4; Potential
	Transmembrane	201 – 221	21	Helical; Name=5; Potential
	Transmembrane	241 – 261	21	Helical; Name=6; Potential
	Motif	112 – 114	3	NPA 1
	Motif	227 – 229	3	NPA 2
	Compositional bias	36 – 42	7	Poly-Ala
Experimental info
	Sequence conflict	190	1	A → V in AK106825. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9ASI1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E3E1A7FD2C9FE54E	FASTA	286	29,806
	10 20 30 40 50 60 MTTDHAGKKV DVVVVGNVDG EHVGVEQARH DLHEEAAAAA AADHHATRGL AIGFLIREVM 70 80 90 100 110 120 VEGLASFLVV FWSCVAALMQ EMYGTLTFPM VCLVVAMTVA FVLSWLGPAH FNPAVTITFA 130 140 150 160 170 180 AYRRFPVWPK LPLYVAAQLA GSLLACLSVN AVMRPRHDHF YGTAPVVVHG TRLPFLMEFL 190 200 210 220 230 240 ASAVLMIVIA TVATDGTAGK TVGGIAIGAA VGGLGLVIGP VSGGSMNPAR TLGPAIVLGR 250 260 270 280 YDGVWIYVVA PVAGMLVGAL CNRAVRLSHR IVAFLCGTSV GIAGSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Identification of 33 rice aquaporin genes and analysis of their expression and function." Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M. Plant Cell Physiol. 46:1568-1577(2005) [PubMed: 16033806] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002882 Genomic DNA. Translation: BAB39896.1. AP003219 Genomic DNA. Translation: BAB61180.1. AP008207 Genomic DNA. Translation: BAF03727.1. AK106825 mRNA. No translation available.
RefSeq	NP_001041813.1. NM_001048348.1.
UniGene	Os.31744.
3D structure databases
HSSP	HSSP built from PDB template 2B5F based on UniProtKB Q41372.
ProteinModelPortal	Q9ASI1.
ModBase	Search...
Proteomic databases
PRIDE	Q9ASI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g02190.1; LOC_Os01g02190.1; LOC_Os01g02190.
GeneID	4326119.
KEGG	osa:4326119.
NMPDR	fig|39947.1.peg.852.
Organism-specific databases
Gramene	Q9ASI1.
Phylogenomic databases
GeneTree	EPGT00070000028199.
HOGENOM	HBG705794.
OMA	PAHFNPA.
ProtClustDB	CLSN2681755.
Family and domain databases
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K09874.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIP41_ORYSJ

Accession

Primary (citable) accession number: Q9ASI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program