Reviewed, UniProtKB/Swiss-Prot Q9ARI6 (CMT2_MAIZE)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase 2
    EC=2.1.1.37
Alternative name(s):
    Chromomethylase 2
    Zea methyltransferase5
      Short name=Zmet5
    DNA cytosine methyltransferase MET5

Gene names

Name:

ZMET5

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	915 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the CpXpG methylation and in gene silencing By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 BAH domain. Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 915	915	DNA (cytosine-5)-methyltransferase 2		PRO_0000246695
Regions
	Domain	188 – 313	126	BAH
	Domain	445 – 508	64	Chromo

Sequences

	Sequence		Length	Mass (Da)
	Q9ARI6-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4BA29AC625D076B2	FASTA	915	101,641
	10 20 30 40 50 60 MAPSSPSSAR PTRASGRERS AMAEEIHQNQ EEEEEVVAAS TAKRRRKAAS SGKKPKPTPK 70 80 90 100 110 120 QAKPAVAGMK KKGETEKTEP VVDDVCAEEP DEEELAMGEE EAEAEEQAMQ EVVAAVAAGS 130 140 150 160 170 180 PGKKRVGRRS AAASGDHVPE FIGSPVGAAE AHSNWPKRYE RSTAANKPEE DDELKARCHY 190 200 210 220 230 240 RSAKVDNIVY CLGDDVYVKA GENEADYIGR ITEFFEGTDR CHYFTCRWFF RAEDTVINSL 250 260 270 280 290 300 VSINVDGHKH DPRRVFLSEE KNDNVLDCII SKVKIVHVDP NMDPKAKAQL IEHCDLYYDM 310 320 330 340 350 360 SYSVAYSTFA NISSENGQSG SETASGISSD DAGLETSSNM PERTATLLDL YSGCGGMSTG 370 380 390 400 410 420 LCLGAALSGL KLETRWAVDL NSFACQSLKY NHPQTEVRNE KADEFLALLK EWAVLCEKYV 430 440 450 460 470 480 HQDVDSNLAG SEDQEDADTL DKDEFVVQKL IGIRYDGTGR KKGVYFKVQW EEYGPEEDTW 490 500 510 520 530 540 EPIDNLSDCP LKIREFVQEG RKRKILPLPG DVDVICGGPP CQGISGFNRF RNRDEPLKDE 550 560 570 580 590 600 KNKQMVTFMD IVAYLKPKYV LMENVVDILK FADGYLGKYA LSCLVAMKYQ ARLGMMVAGC 610 620 630 640 650 660 YGLPQFRMRV FLWGALSSMV LPKYPLPTYD VVVRGGAPNA FSQCMVAYDE TQRPSLKKAL 670 680 690 700 710 720 LLGDAFSDLP KVENHQPNDV MEYGGSPKTE FQRYIRLGRK DMLDWSFGEE AGPDEGKLLD 730 740 750 760 770 780 HQPLRLNNDD YERVKQIPVK KGANFRDLKG VKVGANNVVE WDPEVERVYL SSGKPLVPDY 790 800 810 820 830 840 AMSFIKGKSL KPFGRLWWDQ TVPTVVTRAE PHNQVILHPT QARVLTIREN ARLQGFPDYY 850 860 870 880 890 900 RLFGPIKEKY IQVGNAVAVP VARALGYCLG QAYLGESDGS QPLYQLPASF TSVGRTAVQA 910 NAASVGTPAG EVVEQ

References

[1]	"Maize chromomethylase Zea methyltransferase2 is required for CpNpG methylation." Papa C.M., Springer N.M., Muszynski M.G., Meeley R., Kaeppler S.M. Plant Cell 13:1919-1928(2001) [PubMed: 11487702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY027539 mRNA. Translation: AAK15805.1.
RefSeq	NP_001105167.1.
UniGene	Zm.599
3D structure databases
HSSP	HSSP built from PDB template 1AP0 based on UniProtKB P83917.
ModBase	Search...
Genome annotation databases
GeneID	542060.
Organism-specific databases
Gramene	Q9ARI6.
Enzyme and pathway databases
BRENDA	2.1.1.37. 289.
Family and domain databases
InterPro	IPR001025. BAH. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR000953. Chromodomain. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF01426. BAH. 1 hit. PF00385. Chromo. 1 hit. PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view]
PROSITE	PS51038. BAH. 1 hit. PS00094. C5_MTASE_1. 1 hit. PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMT2_MAIZE

Accession

Primary (citable) accession number: Q9ARI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2001
Last modified:	September 22, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)