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Protein

Isoprene synthase, chloroplastic

Gene

ISPS

Organism
Populus canescens (Grey poplar) (Populus tremula x Populus alba)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate.

Catalytic activityi

Dimethylallyl diphosphate = isoprene + diphosphate.1 Publication

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi345 – 3451Magnesium or manganese 1By similarity
Metal bindingi345 – 3451Magnesium or manganese 2By similarity
Metal bindingi349 – 3491Magnesium or manganese 1By similarity
Metal bindingi349 – 3491Magnesium or manganese 2By similarity
Metal bindingi489 – 4891Magnesium or manganese 3By similarity
Metal bindingi493 – 4931Magnesium or manganese 3By similarity
Metal bindingi497 – 4971Magnesium or manganese 3By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.3.27. 4978.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoprene synthase, chloroplastic (EC:4.2.3.27)
Gene namesi
Name:ISPS
OrganismiPopulus canescens (Grey poplar) (Populus tremula x Populus alba)
Taxonomic identifieri80863 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737ChloroplastSequence analysisAdd
BLAST
Chaini38 – 595558Isoprene synthase, chloroplasticPRO_0000398180Add
BLAST

Structurei

Secondary structure

1
595
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi67 – 704Combined sources
Helixi80 – 9819Combined sources
Helixi104 – 11613Combined sources
Helixi120 – 1223Combined sources
Helixi124 – 13613Combined sources
Helixi139 – 1435Combined sources
Turni144 – 1463Combined sources
Helixi148 – 16013Combined sources
Helixi167 – 1737Combined sources
Beta strandi176 – 1783Combined sources
Helixi182 – 1865Combined sources
Helixi188 – 19811Combined sources
Helixi206 – 22015Combined sources
Helixi224 – 2274Combined sources
Helixi229 – 24012Combined sources
Turni243 – 2453Combined sources
Helixi248 – 25912Combined sources
Helixi267 – 29832Combined sources
Helixi300 – 3034Combined sources
Helixi311 – 32111Combined sources
Helixi325 – 3273Combined sources
Helixi328 – 34922Combined sources
Helixi354 – 36613Combined sources
Helixi369 – 3746Combined sources
Helixi377 – 40125Combined sources
Helixi406 – 42823Combined sources
Helixi435 – 44511Combined sources
Helixi448 – 45912Combined sources
Helixi465 – 4728Combined sources
Helixi476 – 49823Combined sources
Turni499 – 5013Combined sources
Helixi506 – 5149Combined sources
Helixi518 – 54124Combined sources
Helixi548 – 56417Combined sources
Helixi574 – 58512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N0FX-ray2.70A/B53-595[»]
3N0GX-ray2.80A/B53-595[»]
ProteinModelPortaliQ9AR86.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AR86.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi345 – 3495DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsb subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK12742.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9AR86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATELLCLHR PISLTHKLFR NPLPKVIQAT PLTLKLRCSV STENVSFTET
60 70 80 90 100
ETEARRSANY EPNSWDYDFL LSSDTDESIE VYKDKAKKLE AEVRREINNE
110 120 130 140 150
KAEFLTLLEL IDNVQRLGLG YRFESDIRRA LDRFVSSGGF DGVTKTSLHA
160 170 180 190 200
TALSFRLLRQ HGFEVSQEAF SGFKDQNGNF LENLKEDTKA ILSLYEASFL
210 220 230 240 250
ALEGENILDE ARVFAISHLK ELSEEKIGKE LAEQVNHALE LPLHRRTQRL
260 270 280 290 300
EAVWSIEAYR KKEDANQVLL ELAILDYNMI QSVYQRDLRE TSRWWRRVGL
310 320 330 340 350
ATKLHFAKDR LIESFYWAVG VAFEPQYSDC RNSVAKMFSF VTIIDDIYDV
360 370 380 390 400
YGTLDELELF TDAVERWDVN AINDLPDYMK LCFLALYNTI NEIAYDNLKD
410 420 430 440 450
KGENILPYLT KAWADLCNAF LQEAKWLYNK STPTFDDYFG NAWKSSSGPL
460 470 480 490 500
QLIFAYFAVV QNIKKEEIEN LQKYHDIISR PSHIFRLCND LASASAEIAR
510 520 530 540 550
GETANSVSCY MRTKGISEEL ATESVMNLID ETCKKMNKEK LGGSLFAKPF
560 570 580 590
VETAINLARQ SHCTYHNGDA HTSPDELTRK RVLSVITEPI LPFER
Length:595
Mass (Da):68,386
Last modified:June 1, 2001 - v1
Checksum:i4A5826BDCA5903F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294819 mRNA. Translation: CAC35696.1.

Genome annotation databases

KEGGiag:CAC35696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294819 mRNA. Translation: CAC35696.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N0FX-ray2.70A/B53-595[»]
3N0GX-ray2.80A/B53-595[»]
ProteinModelPortaliQ9AR86.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAC35696.

Phylogenomic databases

KOiK12742.

Enzyme and pathway databases

BRENDAi4.2.3.27. 4978.

Miscellaneous databases

EvolutionaryTraceiQ9AR86.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPS_POPCN
AccessioniPrimary (citable) accession number: Q9AR86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.