Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoprene synthase, chloroplastic

Gene

ISPS

Organism
Populus canescens (Grey poplar) (Populus tremula x Populus alba)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate.

Catalytic activityi

Dimethylallyl diphosphate = isoprene + diphosphate.1 Publication

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi345Magnesium or manganese 1By similarity1
Metal bindingi345Magnesium or manganese 2By similarity1
Metal bindingi349Magnesium or manganese 1By similarity1
Metal bindingi349Magnesium or manganese 2By similarity1
Metal bindingi489Magnesium or manganese 3By similarity1
Metal bindingi493Magnesium or manganese 3By similarity1
Metal bindingi497Magnesium or manganese 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.3.27. 4978.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoprene synthase, chloroplastic (EC:4.2.3.27)
Gene namesi
Name:ISPS
OrganismiPopulus canescens (Grey poplar) (Populus tremula x Populus alba)
Taxonomic identifieri80863 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastSequence analysisAdd BLAST37
ChainiPRO_000039818038 – 595Isoprene synthase, chloroplasticAdd BLAST558

Structurei

Secondary structure

1595
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi67 – 70Combined sources4
Helixi80 – 98Combined sources19
Helixi104 – 116Combined sources13
Helixi120 – 122Combined sources3
Helixi124 – 136Combined sources13
Helixi139 – 143Combined sources5
Turni144 – 146Combined sources3
Helixi148 – 160Combined sources13
Helixi167 – 173Combined sources7
Beta strandi176 – 178Combined sources3
Helixi182 – 186Combined sources5
Helixi188 – 198Combined sources11
Helixi206 – 220Combined sources15
Helixi224 – 227Combined sources4
Helixi229 – 240Combined sources12
Turni243 – 245Combined sources3
Helixi248 – 259Combined sources12
Helixi267 – 298Combined sources32
Helixi300 – 303Combined sources4
Helixi311 – 321Combined sources11
Helixi325 – 327Combined sources3
Helixi328 – 349Combined sources22
Helixi354 – 366Combined sources13
Helixi369 – 374Combined sources6
Helixi377 – 401Combined sources25
Helixi406 – 428Combined sources23
Helixi435 – 445Combined sources11
Helixi448 – 459Combined sources12
Helixi465 – 472Combined sources8
Helixi476 – 498Combined sources23
Turni499 – 501Combined sources3
Helixi506 – 514Combined sources9
Helixi518 – 541Combined sources24
Helixi548 – 564Combined sources17
Helixi574 – 585Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N0FX-ray2.70A/B53-595[»]
3N0GX-ray2.80A/B53-595[»]
ProteinModelPortaliQ9AR86.
SMRiQ9AR86.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AR86.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi345 – 349DDXXD motif5

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsb subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK12742.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9AR86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATELLCLHR PISLTHKLFR NPLPKVIQAT PLTLKLRCSV STENVSFTET
60 70 80 90 100
ETEARRSANY EPNSWDYDFL LSSDTDESIE VYKDKAKKLE AEVRREINNE
110 120 130 140 150
KAEFLTLLEL IDNVQRLGLG YRFESDIRRA LDRFVSSGGF DGVTKTSLHA
160 170 180 190 200
TALSFRLLRQ HGFEVSQEAF SGFKDQNGNF LENLKEDTKA ILSLYEASFL
210 220 230 240 250
ALEGENILDE ARVFAISHLK ELSEEKIGKE LAEQVNHALE LPLHRRTQRL
260 270 280 290 300
EAVWSIEAYR KKEDANQVLL ELAILDYNMI QSVYQRDLRE TSRWWRRVGL
310 320 330 340 350
ATKLHFAKDR LIESFYWAVG VAFEPQYSDC RNSVAKMFSF VTIIDDIYDV
360 370 380 390 400
YGTLDELELF TDAVERWDVN AINDLPDYMK LCFLALYNTI NEIAYDNLKD
410 420 430 440 450
KGENILPYLT KAWADLCNAF LQEAKWLYNK STPTFDDYFG NAWKSSSGPL
460 470 480 490 500
QLIFAYFAVV QNIKKEEIEN LQKYHDIISR PSHIFRLCND LASASAEIAR
510 520 530 540 550
GETANSVSCY MRTKGISEEL ATESVMNLID ETCKKMNKEK LGGSLFAKPF
560 570 580 590
VETAINLARQ SHCTYHNGDA HTSPDELTRK RVLSVITEPI LPFER
Length:595
Mass (Da):68,386
Last modified:June 1, 2001 - v1
Checksum:i4A5826BDCA5903F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294819 mRNA. Translation: CAC35696.1.

Genome annotation databases

KEGGiag:CAC35696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294819 mRNA. Translation: CAC35696.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N0FX-ray2.70A/B53-595[»]
3N0GX-ray2.80A/B53-595[»]
ProteinModelPortaliQ9AR86.
SMRiQ9AR86.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAC35696.

Phylogenomic databases

KOiK12742.

Enzyme and pathway databases

BRENDAi4.2.3.27. 4978.

Miscellaneous databases

EvolutionaryTraceiQ9AR86.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPS_POPCN
AccessioniPrimary (citable) accession number: Q9AR86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.