Reviewed, UniProtKB/Swiss-Prot Q9AR74 (TCMO_RUTGR)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    Cytochrome P450C4H
    Cytochrome P450 73

Gene names

Name:

CYP73A2

Organism

Ruta graveolens (Common rue)

Taxonomic identifier

37565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Sapindales › Rutaceae › Ruta

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins By similarity.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Trans-cinnamate 4-monooxygenase		PRO_0000052253
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AR74-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7609D7508B9803D9	FASTA	506	58,105
	10 20 30 40 50 60 MDLLLLEKAL LGLFAAAVVA IAVSKLRGKR FKLPPGPLGF PVFGNWLQVG DDLNQRKLAN 70 80 90 100 110 120 LSKKFGDVYL LRMGQRNLVV VSSPEMAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYSEHWRKMR RIMTVPFFTN KVVQQQRFNW EDEAARVVED VKKDPQAATT GIVLRRRLQL 190 200 210 220 230 240 LMYNNMYRIM FDRRFESVDD PLFNKLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK 250 260 270 280 290 300 LVKEVKERRL KLFKDYFVEE RKKLTSTKSM TEENFKCAID HVLDAQQKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPDIQK KLRAEIDRVL GPDHQITEPD THKLPYLQAV 370 380 390 400 410 420 IKETLRLRMA IPLLVPHMNL NDAKLAGYDI PAESKILVNA WWLANNPAHW KDPQVFRPER 430 440 450 460 470 480 FLEEESGVEA NGNDFRYIPF GVGRRSCPGI ILALPILGIT IGRMVQNFEL LPPPGQSKID 490 500 TSEKGGQFSL FILNHSTIVL KPRSSV

References

[1]	"Molecular cloning and expression of Ruta graveolens cinnamate 4-hydroxylase cDNA." Gravot A., Gontier E., Bourgaud F., Deleury E., Goergen J.L. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ309127 mRNA. Translation: CAC35977.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 2152.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_RUTGR

Accession

Primary (citable) accession number: Q9AR74

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)