Reviewed, UniProtKB/Swiss-Prot Q9AR22 (CTH1_CHLRE)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 2
    EC=1.14.13.81
Alternative name(s):
    Copper target homolog 1 protein

Gene names

Name:

CTH1

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.1
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Induction	By Copper and oxygen. Ref.1
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 407		Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2, chloroplastic		PRO_0000000600
Experimental info
	Sequence conflict	18	1	P → L in AAL14712. Ref.1
	Sequence conflict	38	1	A → V in AAL14712. Ref.1
	Sequence conflict	356	1	N → D in AAL14712. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9AR22-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4C3A2920C158AA50	FASTA	407	47,482
	10 20 30 40 50 60 MATMLNKRVT GAFGKQAPVR SARVAAVRPS RSSVRVAATA APQEVEGFKV MRDGIKVASD 70 80 90 100 110 120 ETLLTPRFYT TDFDEMERLF SLELNKNMDM EEFEAMLNEF KLDYNQRHFV RNETFKEAAE 130 140 150 160 170 180 KIQGPTRKIF IEFLERSCTA EFSGFLLYKE LGRRLKATNP VVAEIFTLMS RDEARHAGFL 190 200 210 220 230 240 NKAMSDFNLA LDLGFLTKNR KYTFFKPKFI FYATYLSEKI GYWRYISIYR HLQRNPDNQL 250 260 270 280 290 300 YPLFEYFENW CQDENRHGDF FTAVLKARPE MVNDWAAKLW SRFFCLSVYI TMYLNDHQRD 310 320 330 340 350 360 AFYSSLGLNT TQFNQHVIIE TNKSTERIFP AVPDVENPEF FRRMDLLVKY NAQLVNIGSM 370 380 390 400 NLPSPIKAIM KAPILERMVA EVFQVFIMTP KESGSYDLDA NKTALVY

References

[1]

"Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation." Moseley J.L., Page M.D., Alder N.P., Eriksson M., Quinn J., Soto F., Theg S.M., Hippler M., Merchant S. Plant Cell 14:673-688(2002) [PubMed: 11910013] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF337037 mRNA. Translation: AAK32149.1. AF426027 mRNA. Translation: AAL14712.2. AF337038 Genomic DNA. Translation: AAK32150.1.
RefSeq	XP_001691047.1. XP_001691048.1.
UniGene	Cre.4405
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9AR22.
Genome annotation databases
GeneID	5716556.
KEGG	cre:CHLREDRAFT_205856.
Enzyme and pathway databases
BRENDA	1.14.13.81. 144.
Family and domain databases
InterPro	IPR008434. AcsF. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CTH1_CHLRE

Accession

Primary (citable) accession number: Q9AR22
Secondary accession number(s): Q944P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)