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Q9AR22 (CTH1_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2, chloroplastic
Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 2
EC=1.14.13.81
Alternative name(s):
Copper target homolog 1 protein
Gene names
Name:CTH1
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.1

Catalytic activity

Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O.

131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O.

131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.

Cofactor

Iron By similarity.

Pathway

Porphyrin biosynthesis; chlorophyll biosynthesis.

Subcellular location

Plastid › chloroplast By similarity.

Induction

By Copper and oxygen. Ref.1

Sequence similarities

Belongs to the AcsF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 407Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2, chloroplasticPRO_0000000600

Experimental info

Sequence conflict181P → L in AAL14712. Ref.1
Sequence conflict381A → V in AAL14712. Ref.1
Sequence conflict3561N → D in AAL14712. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9AR22 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 4C3A2920C158AA50

FASTA40747,482
        10         20         30         40         50         60 
MATMLNKRVT GAFGKQAPVR SARVAAVRPS RSSVRVAATA APQEVEGFKV MRDGIKVASD 

        70         80         90        100        110        120 
ETLLTPRFYT TDFDEMERLF SLELNKNMDM EEFEAMLNEF KLDYNQRHFV RNETFKEAAE 

       130        140        150        160        170        180 
KIQGPTRKIF IEFLERSCTA EFSGFLLYKE LGRRLKATNP VVAEIFTLMS RDEARHAGFL 

       190        200        210        220        230        240 
NKAMSDFNLA LDLGFLTKNR KYTFFKPKFI FYATYLSEKI GYWRYISIYR HLQRNPDNQL 

       250        260        270        280        290        300 
YPLFEYFENW CQDENRHGDF FTAVLKARPE MVNDWAAKLW SRFFCLSVYI TMYLNDHQRD 

       310        320        330        340        350        360 
AFYSSLGLNT TQFNQHVIIE TNKSTERIFP AVPDVENPEF FRRMDLLVKY NAQLVNIGSM 

       370        380        390        400 
NLPSPIKAIM KAPILERMVA EVFQVFIMTP KESGSYDLDA NKTALVY

« Hide

References

[1]"Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation."
Moseley J.L., Page M.D., Alder N.P., Eriksson M., Quinn J., Soto F., Theg S.M., Hippler M., Merchant S.
Plant Cell 14:673-688(2002) [PubMed: 11910013] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF337037 mRNA. Translation: AAK32149.1.
AF426027 mRNA. Translation: AAL14712.2.
AF337038 Genomic DNA. Translation: AAK32150.1.
RefSeqXP_001691047.1. XM_001690995.1.
XP_001691048.1. XM_001690996.1.
UniGeneCre.4405.

3D structure databases

ProteinModelPortalQ9AR22.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9AR22.

Proteomic databases

PRIDEQ9AR22.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsEDP05493; EDP05494; CHLREDRAFT_205856.
EDP05494; EDP05493; CHLREDRAFT_205856.
GeneID5716556.
KEGGcre:CHLREDRAFT_205856.

Phylogenomic databases

PhylomeDBQ9AR22.
ProtClustDBPLN02508.

Family and domain databases

InterProIPR008434. AcsF.
IPR012347. Ferritin-rel.
IPR009078. Ferritin/RR-like.
IPR003251. Rubrerythrin.
[Graphical view]
Gene3DG3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KOK04035.
PfamPF02915. Rubrerythrin. 1 hit.
[Graphical view]
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMsTIGR02029. AcsF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCTH1_CHLRE
AccessionPrimary (citable) accession number: Q9AR22
Secondary accession number(s): Q944P4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2001
Last modified: December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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