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Protein

Histone acetyltransferase GCN5

Gene

HAG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acetylates histone H3 and ADA2 proteins in vitro. Acetylates 'Lys-14' of histone H3. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators. Acts via the formation of large multiprotein complexes that modify the chromatin (By similarity).By similarity5 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Enzyme regulationi

Histone acetyltransferase activity stimulated by the presence of ADA2 proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei289Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • acetyltransferase activity Source: TAIR
  • DNA binding Source: TAIR
  • H3 histone acetyltransferase activity Source: TAIR
  • histone acetyltransferase activity Source: TAIR

GO - Biological processi

  • flower development Source: TAIR
  • histone acetylation Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • regulation of vegetative phase change Source: TAIR
  • response to light stimulus Source: TAIR
  • root morphogenesis Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BRENDAi2.3.1.48. 399.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase GCN5 (EC:2.3.1.48)
Short name:
AtGCN5
Alternative name(s):
BIG TOP protein
Gene namesi
Name:HAG1
Synonyms:BGT, GCN5, HAT1
Ordered Locus Names:At3g54610
ORF Names:T14E10.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54610.

Subcellular locationi

GO - Cellular componenti

  • histone acetyltransferase complex Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Pleiotropic effects on plant growth and development, including dwarf size, aberrant root development, and short petals and stamens in flowers. No embryonic phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi478 – 568Missing in bgt-1/hag1-3; no embryonic phenotypes, but suppresses the tpl-1 phenotype. 1 PublicationAdd BLAST91

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002697481 – 568Histone acetyltransferase GCN5Add BLAST568

Proteomic databases

PaxDbiQ9AR19.

PTM databases

iPTMnetiQ9AR19.

Expressioni

Tissue specificityi

Expressed in roots and leaves.1 Publication

Gene expression databases

GenevisibleiQ9AR19. AT.

Interactioni

Subunit structurei

Interacts in vitro with ADA2a, ADA2b and the transcriptional activator DREB1B/CBF1. Interacts with PP2C1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADA2BQ9ATB45EBI-979271,EBI-979237

Protein-protein interaction databases

BioGridi9942. 28 interactors.
IntActiQ9AR19. 3 interactors.
STRINGi3702.AT3G54610.1.

Structurei

3D structure databases

ProteinModelPortaliQ9AR19.
SMRiQ9AR19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 371N-acetyltransferasePROSITE-ProRule annotationAdd BLAST150
Domaini472 – 543BromoPROSITE-ProRule annotationAdd BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni293 – 295Acetyl-CoA bindingBy similarity3
Regioni300 – 306Acetyl-CoA bindingBy similarity7
Regioni332 – 335Acetyl-CoA bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 66Ser-richAdd BLAST64
Compositional biasi89 – 116Asp-richAdd BLAST28

Domaini

The N-terminal part (1-150) is not required for interactions with the ADA2 proteins.

Sequence similaritiesi

Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG1472. Eukaryota.
COG5076. LUCA.
HOGENOMiHOG000192257.
InParanoidiQ9AR19.
KOiK06062.
OMAiRLKFVCL.
OrthoDBiEOG093608QI.
PhylomeDBiQ9AR19.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
PF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AR19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSHSSHLNA ANRSRSSQTP SPSHSASASV TSSLHKRKLA ATTAANAAAS
60 70 80 90 100
EDHAPPSSSF PPSSFSADTR DGALTSNDEL ESISARGADT DSDPDESEDI
110 120 130 140 150
VVDDDEDEFA PEQDQDSSIR TFTAARLDSS SGVNGSSRNT KLKTESSTVK
160 170 180 190 200
LESSDGGKDG GSSVVGTGVS GTVGGSSISG LVPKDESVKV LAENFQTSGA
210 220 230 240 250
YIAREEALKR EEQAGRLKFV CYSNDSIDEH MMCLIGLKNI FARQLPNMPK
260 270 280 290 300
EYIVRLLMDR KHKSVMVLRG NLVVGGITYR PYHSQKFGEI AFCAITADEQ
310 320 330 340 350
VKGYGTRLMN HLKQHARDVD GLTHFLTYAD NNAVGYFVKQ GFTKEIYLEK
360 370 380 390 400
DVWHGFIKDY DGGLLMECKI DPKLPYTDLS SMIRQQRKAI DERIRELSNC
410 420 430 440 450
QNVYPKIEFL KNEAGIPRKI IKVEEIRGLR EAGWTPDQWG HTRFKLFNGS
460 470 480 490 500
ADMVTNQKQL NALMRALLKT MQDHADAWPF KEPVDSRDVP DYYDIIKDPI
510 520 530 540 550
DLKVIAKRVE SEQYYVTLDM FVADARRMFN NCRTYNSPDT IYYKCATRLE
560
THFHSKVQAG LQSGAKSQ
Length:568
Mass (Da):63,124
Last modified:June 1, 2001 - v1
Checksum:i505C4A1816C376CC
GO

Sequence cautioni

The sequence AAB92257 differs from that shown. Reason: Frameshift at positions 42, 163 and 548.Curated
The sequence CAB77581 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68D → S in AAB92257 (Ref. 1) Curated1
Sequence conflicti230H → R in AAB92257 (Ref. 1) Curated1
Sequence conflicti243R → G in AAB92257 (Ref. 1) Curated1
Sequence conflicti247N → K in AAB92257 (Ref. 1) Curated1
Sequence conflicti247N → K in AAB87070 (Ref. 1) Curated1
Sequence conflicti363G → A in AAB92257 (Ref. 1) Curated1
Sequence conflicti363G → A in AAB87070 (Ref. 1) Curated1
Sequence conflicti365L → P in AAB92257 (Ref. 1) Curated1
Sequence conflicti474H → R in AAB92257 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037442 mRNA. Translation: AAB92257.1. Frameshift.
AF031958 mRNA. Translation: AAB87070.1.
AF338768 mRNA. Translation: AAK31318.1.
AF338771 Genomic DNA. Translation: AAK31321.1.
AL138656 Genomic DNA. Translation: CAB77581.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79255.1.
BT008909 mRNA. Translation: AAP68348.1.
BT002057 mRNA. Translation: AAN72068.1.
PIRiT47620.
RefSeqiNP_567002.1. NM_115318.3.
UniGeneiAt.10490.

Genome annotation databases

EnsemblPlantsiAT3G54610.1; AT3G54610.1; AT3G54610.
GeneIDi824626.
GrameneiAT3G54610.1; AT3G54610.1; AT3G54610.
KEGGiath:AT3G54610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037442 mRNA. Translation: AAB92257.1. Frameshift.
AF031958 mRNA. Translation: AAB87070.1.
AF338768 mRNA. Translation: AAK31318.1.
AF338771 Genomic DNA. Translation: AAK31321.1.
AL138656 Genomic DNA. Translation: CAB77581.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79255.1.
BT008909 mRNA. Translation: AAP68348.1.
BT002057 mRNA. Translation: AAN72068.1.
PIRiT47620.
RefSeqiNP_567002.1. NM_115318.3.
UniGeneiAt.10490.

3D structure databases

ProteinModelPortaliQ9AR19.
SMRiQ9AR19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9942. 28 interactors.
IntActiQ9AR19. 3 interactors.
STRINGi3702.AT3G54610.1.

PTM databases

iPTMnetiQ9AR19.

Proteomic databases

PaxDbiQ9AR19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54610.1; AT3G54610.1; AT3G54610.
GeneIDi824626.
GrameneiAT3G54610.1; AT3G54610.1; AT3G54610.
KEGGiath:AT3G54610.

Organism-specific databases

TAIRiAT3G54610.

Phylogenomic databases

eggNOGiKOG1472. Eukaryota.
COG5076. LUCA.
HOGENOMiHOG000192257.
InParanoidiQ9AR19.
KOiK06062.
OMAiRLKFVCL.
OrthoDBiEOG093608QI.
PhylomeDBiQ9AR19.

Enzyme and pathway databases

BRENDAi2.3.1.48. 399.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiQ9AR19.

Gene expression databases

GenevisibleiQ9AR19. AT.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
PF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCN5_ARATH
AccessioniPrimary (citable) accession number: Q9AR19
Secondary accession number(s): O22654, Q7G1G9, Q9M1G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in HAG1 suppress the tpl-1 phenotype showing a transformation during embryogenesis of the shoot pole into a second root pole.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.