Reviewed, UniProtKB/Swiss-Prot Q9AQA0 (ILVC_BUCUM)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:

ilvC

Organism

Buchnera aphidicola subsp. Uroleucon ambrosiae

Taxonomic identifier

118117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	346 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›346	›346	Ketol-acid reductoisomerase		PRO_0000151295
Sites
	Active site	65	1	Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	346	1

Sequences

	Sequence		Length	Mass (Da)
	Q9AQA0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 77BDE5FF866785BA	FASTA	346	39,458
	10 20 30 40 50 60 KKNSILKKNQ SWINATENNF KVDDYESLIP NADLVINLTP DKEHHNVVTT LQKLMKKNSY 70 80 90 100 110 120 LGYSHGFNIV EFGEIIRKDI TVIMVAPKCP GTEVREEYKR GFGVPTLIAV HNENDIHQKG 130 140 150 160 170 180 LEIAKAWSFS IGSHRAGVLE SSFIAEVKSD LMGEQTILCG MLQTASILCY EKLITNKHDP 190 200 210 220 230 240 AYAAKLIQNG WETITESLKH GGITLMMDRL SNPSKIRAYK ISEKIKKILT SLFQQHMNNI 250 260 270 280 290 300 ISGEFSSEMM KDWKNNDKKL LDWRKQTKDT SFEKAPIYIK KIPEQEYYDH GILMIAILKA 310 320 330 340 GIELSFETMI NSGIIAESAY YESLHELPLI ANTIARKKLY EMNKVI

References

[1]	"Accelerated evolutionary rates at biosynthetic loci of Buchnera-Uroleucon." Wernegreen J.J., Moran N.A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF217553 Genomic DNA. Translation: AAK01024.1.
3D structure databases
SMR	Q9AQA0. Positions 1-346.
ModBase	Search...
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCUM

Accession

Primary (citable) accession number: Q9AQA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	June 1, 2001
Last modified:	November 4, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)