Q9AP06 (KATG_BURCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Synonyms:	katA

Organism

Burkholderia cepacia (Pseudomonas cepacia)

Taxonomic identifier

292 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Required for maintaining the normal activity of the TCA cycle. Ref.1
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Catalase-peroxidase HAMAP MF_01961		PRO_0000354738
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253) By similarity
	Cross-link	227 ↔ 253		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AP06 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5ECA94CF843676C4	FASTA	760	83,315
	10 20 30 40 50 60 MSNEGQCPFN HANGGGTTNR DWWPNELRLD LLSQHSSKTD PLDPGFNYAE AFNSLDLDAL 70 80 90 100 110 120 RKDLAALMTD SQDWWPADFG HYGPLFVRMA WHSAGTYRMG DGRGGAGRGQ QRFAPLNSWP 130 140 150 160 170 180 DNVSLDKARR LLWPIKQKYG QKISWADLLI LTGDVALTTM GFKTFGYAGG REDTWEPDRD 190 200 210 220 230 240 VYWGSETTWL GGDLRYDKGG ACESQHGGNA GRNLENPLAA VQMDLIYNPE GGPDGNPDPV 250 260 270 280 290 300 AAAYDIREVF GRMAMNDEET VALIAGGHAF GKTHGAGPAD NVGLEPEAAG LEQQGLGWKN 310 320 330 340 350 360 SFGTGKGADT ITSGLEVTWS DTPTQWGMGF FKNLFGYEWE LTKSPAGAHQ WVAKNAEPTI 370 380 390 400 410 420 PHAHDPSKKL LPTMLTTDLS LRFDPVYEKI SRHFMDNPDV FADAFARAWF KLTHRDMGPR 430 440 450 460 470 480 ARYLGPDVPT EELIWQDPIP AVDHVLTTRN VAPLKETILA SGLSVAELVS TAWASASTFR 490 500 510 520 530 540 GSDKRGGANG ARIRLAPQKD WAVNEPARLA KVLKVLERIQ GEFNSTQPGG KKISLADLIV 550 560 570 580 590 600 LAGGAGIEQA AKRAGHDVVV PFAPGRMDAS QEQTDAHSFA VLEPVADGFR NFVKGKFAVP 610 620 630 640 650 660 AEALLIDKAQ LLTLTAPQMT ALVGGLRVLN VQTGDEKHGV FTDQPETLTV DFFRNLLDMA 670 680 690 700 710 720 TEWKPIAGED TYEGRDRRTG ELKWTGTRVD LVFGSNAVLR ALSEVYASAD GEAKFIRDFV 730 740 750 760 AAWVKVMNLD RFDLACKKRW YIDASGLPGE PALSARIQWR

References

[1]	"A minor catalase/peroxidase from Burkholderia cenocepacia is required for normal aconitase activity." Lefebre M.D., Flannagan R.S., Valvano M.A. Microbiology 151:1975-1985(2005) [PubMed: 15942004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: C5424.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF317697 Genomic DNA. Translation: AAG60688.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q9AP06.
SMR	Q9AP06. Positions 16-735.
ModBase	Search...
Protein family/group databases
PeroxiBase	2323. BcCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BURCE

Accession

Primary (citable) accession number: Q9AP06

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 1, 2001
Last modified:	October 19, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program