Reviewed, UniProtKB/Swiss-Prot Q9AMV8 (METE_BRAJA)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	blr2068
ORF Names:	id830

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 781	781	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098618
Sites
	Metal binding	663	1	Zinc By similarity
	Metal binding	665	1	Zinc By similarity
	Metal binding	748	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AMV8-1 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: 496DE1A22509E8E8	FASTA	781	87,402
	10 20 30 40 50 60 MSPLSLPVAT LGTPRIGPRR ELKLALESYW AGKISEQQLH EDAFGLRAAN WARQKSLGVT 70 80 90 100 110 120 IIPSNDFSLY DQVLDTSVMV GAIPEIYAAN GESVSLQTYF AMARGSQCGE QDASCAQSPR 130 140 150 160 170 180 GSGVPAQEMT KWFDTNYHYM VPEFYRGQAF RLCSRKPVEE YEEARALGFQ TRPVLIGPVT 190 200 210 220 230 240 FLKLGKSTDS AFQPLSLLDD LIPVYIDLLH ELAKRGASWV QFDEPCLVLD LDEAARNSLL 250 260 270 280 290 300 HAYNRFAKEG PAIKIMLASY FGALGDNLDT ALSLPISGLH VDLVRAPELL DQIVADGRSD 310 320 330 340 350 360 LVMSLGVIDG RNVWRSNLPS LRQRLKPSIA KLGRHRVQLA PSCSLLHVPV DVELETGLAS 370 380 390 400 410 420 DVKSWLAFSV QKMRELAILA RVLAGDQNVG LALAESECAA TARRTSPKIH NANVAVRMRA 430 440 450 460 470 480 IDQTMRQRAT PFARRSEIQR ERFGLPAFPT TTIGSFPQTT AVRNARAAHA RGAMSEEQYD 490 500 510 520 530 540 RFLKEEIARA VRWQEDIGLD VLVHGEFERN DMVQYFSEQL AGFAFTRNGW VQSYGSRCVR 550 560 570 580 590 600 PPILFGDVVR PKPITVEWWR YAQSLTSKPM KAMLTGPVTI LNWSFVRDDI PRSEVCRQLA 610 620 630 640 650 660 LAMRDEVRDL ENAGAAMIQI DEAALREGLP LRRSDWKAYL DWAGDCFRIC SSGVTDQTQI 670 680 690 700 710 720 HTHMCYSEFN DIIGAIAAMD ADVISIETSR SKMELLDAFR RYEYPNQIGP GVYDIHSPRV 730 740 750 760 770 780 PETDEMKELI VLARTRLQDS QLWVNPDCGL KTRKWEEVRP ALANMVAAAR ELRAASDPQI R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA region of the Bradyrhizobium japonicum chromosome." Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R., Hennecke H. J. Bacteriol. 183:1405-1412(2001) [PubMed: 11157954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF322013 Genomic DNA. Translation: AAG61038.1. Different initiation. BA000040 Genomic DNA. Translation: BAC47333.1.
RefSeq	NP_768708.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1055481.
GenomeReviews	Gene locus blr2068 in contig BA000040_GR.
KEGG	bja:blr2068.
NMPDR	fig|224911.1.peg.2068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9AMV8.
OMA	CSLLHTP.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2068-MON.
BRENDA	2.1.1.14. 280.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BRAJA

Accession

Primary (citable) accession number: Q9AMV8
Secondary accession number(s): Q89TE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)