Reviewed, UniProtKB/Swiss-Prot Q9AMR9 (PANC1_BRAJA)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase 1
      Short name=PS 1
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase 1
    Pantoate-activating enzyme 1

Gene names

Name:	panC1
Ordered Locus Names:	blr2102
ORF Names:	id912

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity.
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Pantothenate synthetase 1 HAMAP MF_00158		PRO_0000128208
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
	Nucleotide binding	147 – 150	4	ATP By similarity
	Nucleotide binding	184 – 187	4	ATP By similarity
Sites
	Active site	37	1	Proton donor By similarity
	Binding site	61	1	Beta-alanine By similarity
	Binding site	61	1	Pantoate By similarity
	Binding site	153	1	Pantoate By similarity
	Binding site	176	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AMR9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A2CA526734D9A607	FASTA	283	31,322
	10 20 30 40 50 60 MKVITKVAEL RRALADVRNA EKRIGFVPTM GYLHDGHLAL ISASREHCDV TVVSIFVNPT 70 80 90 100 110 120 QFGPNEDLSR YPRDFARDEA LCGSAGVSII FAPSAEEIYP AQFESFVEPG ELAKPLCGAF 130 140 150 160 170 180 RPGHFRGVAT VVCKLFNMVQ PDVAYFGQKD FQQCAVIRRM TVDLNLPIEI VTVPTVREPD 190 200 210 220 230 240 GLAMSSRNRY LCPEERDRSL AISRGLFAAA HEFASGERDA ATLIALARRH LERVDRLQYL 250 260 270 280 ELVDPGTLRI ADSPLRYPAV LCVAAYVGST RLIDNVVLSW SPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA region of the Bradyrhizobium japonicum chromosome." Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R., Hennecke H. J. Bacteriol. 183:1405-1412(2001) [PubMed: 11157954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF322013 Genomic DNA. Translation: AAG61078.1. BA000040 Genomic DNA. Translation: BAC47367.1.
RefSeq	NP_768742.1.
3D structure databases
HSSP	HSSP built from PDB template 1IHO based on UniProtKB P31663.
ModBase	Search...
Genome annotation databases
GeneID	1055455.
GenomeReviews	Gene locus blr2102 in contig BA000040_GR.
KEGG	bja:blr2102.
NMPDR	fig|224911.1.peg.2102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9AMR9.
OMA	SRNVYLN.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2102-MON.
BRENDA	6.3.2.1. 280.
Family and domain databases
HAMAP	MF_00158. [Tree]
InterPro	IPR004821. Cyt_trans_rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC1_BRAJA

Accession

Primary (citable) accession number: Q9AMR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)