Chitinase A precursor - Vibrio harveyi

Preferred order of sections

Names and origin

Protein names

Submitted name:
Chitinase A EMBL AAK11576.1

Gene names

Name:

chiA EMBL AAK11576.1

Organism

Vibrio harveyi (Beneckea harveyi) EMBL AAK11576.1

Taxonomic identifier

669 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	850 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. RuleBase RU004453

Ontologies

Keywords
Domain	Signal EMBL AAK11576.1
Molecular function	Glycosidase RuleBase RU000489 Hydrolase
Technical term	3D-structure PDB 3B9A PDB 3B9D PDB 3B9E PDB 3B8S PDB 3ARV PDB 3ARY PDB 3ARO PDB 3AS0 PDB 3ARU PDB 3ARX PDB 3ARS PDB 3ARW PDB 3AS1 PDB 3ARP PDB 3ARR PDB 3AS3 PDB 3ART PDB 3ARQ PDB 3AS2 PDB 3ARZ
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential EMBL AAK11576.1

Chain

22 – 850

829

Potential EMBL AAK11576.1

PRO_5000058799

Regions

Region

275 – 276

2

N-acetyl-D-glucosamine binding PDB 3B9A PDB 3B9D

Region

570 – 571

2

N-acetyl-D-glucosamine binding PDB 3B9A PDB 3B9D

Sites

Binding site

164

1

N-acetyl-D-glucosamine PDB 3B9A

Binding site

168

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

173

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

370

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

392

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

397

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

435

1

N-acetyl-D-glucosamine PDB 3B9A PDB 3B9D

Binding site

463

1

N-acetyl-D-glucosamine PDB 3B9A

Binding site

498

1

N-acetyl-D-glucosamine PDB 3B9D

Sequences

Sequence

Length

Mass (Da)

Tools

Q9AMP1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 5C960448826A8DEB
Show »

FASTA

850

90,249

        10         20         30         40         50         60 
MIRFNLCAAG VALALSGAAN AAPTAPSIDM YGSNNLQFSK IELAMETTSG YNDMVKYHEL 

        70         80         90        100        110        120 
AKIKVKFNQW SGTSGDTYNV YFDGVKVATG AITGSQTTAS FEYGQGGLYQ MEIEACDATG 

       130        140        150        160        170        180 
CSKSAPVEIT IADTDGSHLK PLTMNVDPNN KSYNTDPSIV MGTYFVEWGI YGRDYTVDNM 

       190        200        210        220        230        240 
PVDNLTHILY GFIPICGPNE SVKSVGGNSF NALQTACRGV NDYEVVIHDP WAAYQKSFPQ 

       250        260        270        280        290        300 
AGHEYSTPIK GNYAMLMALK QRNPDLKIIP SIGGWTLSDP FYDFVDKKNR DTFVASVKKF 

       310        320        330        340        350        360 
LKTWKFYDGV DIDWEFPGGG GAAADKGDPV NDGPAYIALM RELRVMLDEL EAETGRTYEL 

       370        380        390        400        410        420 
TSAIGVGYDK IEDVDYADAV QYMDYIFAMT YDFYGGWNNV PGHQTALYCG SFMRPGQCDG 

       430        440        450        460        470        480 
GGVDENGEPY KGPAYTADNG IQLLLAQGVP ANKLVLGTAM YGRGWEGVTP DTLTDPNDPM 

       490        500        510        520        530        540 
TGTATGKLKG STAQGVWEDG VIDYKGIKSF MLGANNTGIN GFEYGYDAQA EAPWVWNRST 

       550        560        570        580        590        600 
GELITFDDHR SVLAKGNYAK SLGLAGLFSW EIDADNGDIL NAMHEGMAGG VVTPPNRKPT 

       610        620        630        640        650        660 
AAAGADQAVT GPASVVLDGS NSTDSDGTIA SYAWEQVSGT AVVLSGANTA TASFDAVEVA 

       670        680        690        700        710        720 
AEEQLTFKLT VTDNEGATAS DLVVVTVKPA GVIDPPNTAP VAQVSAPATA SAGDVVVIDA 

       730        740        750        760        770        780 
SASSDADNDT LTFDWTLPQG LNATVNGAKV TFTAAEYPQD TSLSFTVSVS DGQAVSTASA 

       790        800        810        820        830        840 
TVVVAKHSTG GGTCTNAWDA TAVYTGGDQV TYAGKTWEAK WWDSRVKILA SLANGVFGKK 

       850 
LVHLTVTKHC

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References

[1]	"Isolation and Characterization of the gene encoding the precursor of chitinase chiA from Vibrio carchariae." Suginta W., Rigden D.J., Estibeiro P., Duncan R.R., Ketudat-Cairns J., Fothergill-Gilmore L.A. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism." Songsiriritthigul C., Pantoom S., Aguda A.H., Robinson R.C., Suginta W. J. Struct. Biol. 162:491-499(2008) [PubMed: 18467126] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 22-597 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.
[3]	"Potent Family-18 Chitinase Inhibitors: X-Ray Structures, Affinities, and Binding Mechanisms." Pantoom S., Vetter I.R., Prinz H., Suginta W. Submitted (DEC-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 22-597.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF323180 Genomic DNA. Translation: AAK11576.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ARO	X-ray	2.22	A	22-597	[»]
3ARP	X-ray	1.55	A	22-597	[»]
3ARQ	X-ray	1.50	A	22-597	[»]
3ARR	X-ray	1.65	A	22-597	[»]
3ARS	X-ray	2.45	A	22-597	[»]
3ART	X-ray	2.23	A	22-597	[»]
3ARU	X-ray	1.90	A	22-597	[»]
3ARV	X-ray	1.50	A	22-597	[»]
3ARW	X-ray	1.50	A	22-597	[»]
3ARX	X-ray	1.16	A	22-597	[»]
3ARY	X-ray	1.35	A	22-597	[»]
3ARZ	X-ray	1.82	A	22-597	[»]
3AS0	X-ray	2.00	A	22-597	[»]
3AS1	X-ray	2.00	A	22-597	[»]
3AS2	X-ray	1.80	A	22-597	[»]
3AS3	X-ray	2.40	A	22-597	[»]
3B8S	X-ray	2.00	A/B	22-597	[»]
3B9A	X-ray	1.80	A	22-597	[»]
3B9D	X-ray	1.72	A	22-597	[»]
3B9E	X-ray	1.70	A	22-597	[»]

ProteinModelPortal

Q9AMP1.

SMR

Q9AMP1. Positions 22-588.

ModBase

Search...

Protein family/group databases

CAZy

CBM5. Carbohydrate-Binding Module Family 5.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR003610. CBM_fam5/12.
IPR011583. Chitinase_II.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
IPR002859. PKD/REJ-like.
IPR000601. PKD_dom.
[Graphical view]

Gene3D

G3DSA:2.10.10.20. G3DSA:2.10.10.20. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF02839. CBM_5_12. 1 hit.
PF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
PF02010. REJ. 1 hit.
[Graphical view]

SMART

SM00636. Glyco_18. 1 hit.
SM00089. PKD. 3 hits.
[Graphical view]

SUPFAM

SSF51055. CBM_5_12. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF49299. PKD. 1 hit.

PROSITE

PS01095. CHITINASE_18. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9AMP1_VIBHA

Accession

Primary (citable) accession number: Q9AMP1

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)