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Q9ALM4

- Q9ALM4_9PSEU

UniProt

Q9ALM4 - Q9ALM4_9PSEU

Protein
Submitted name:

Polyketide synthase extender modules 3-4

Gene

spnC

Organism
Saccharopolyspora spinosa
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1276 – 12761NADP; via carbonyl oxygenImported
    Binding sitei1300 – 13001NADPImported
    Binding sitei1337 – 13371NADPImported
    Binding sitei1366 – 13661NADP; via amide nitrogenImported
    Binding sitei1371 – 13711NADP; via amide nitrogenImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1204 – 12052NADPImported
    Nucleotide bindingi1225 – 12273NADPImported
    Nucleotide bindingi1254 – 12552NADPImported

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. nucleotide binding Source: UniProtKB-KW
    3. oxidoreductase activity Source: InterPro
    4. phosphopantetheine binding Source: InterPro
    5. transferase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    TransferaseUniRule annotation

    Keywords - Ligandi

    NADPImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Polyketide synthase extender modules 3-4Imported
    Gene namesi
    Name:spnCImported
    OrganismiSaccharopolyspora spinosaImported
    Taxonomic identifieri60894 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

    PTM / Processingi

    Keywords - PTMi

    PhosphopantetheineUniRule annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IMPX-ray2.57A/B/C/D913-1471[»]
    ProteinModelPortaliQ9ALM4.
    SMRiQ9ALM4. Positions 4-910, 3000-3090.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.1200.10. 2 hits.
    3.40.366.10. 4 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 2 hits.
    PF00106. adh_short. 2 hits.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 2 hits.
    PF02801. Ketoacyl-synt_C. 2 hits.
    PF00550. PP-binding. 2 hits.
    [Graphical view]
    SMARTiSM00827. PKS_AT. 2 hits.
    SM00822. PKS_KR. 2 hits.
    SM00825. PKS_KS. 2 hits.
    SM00823. PKS_PP. 2 hits.
    [Graphical view]
    SUPFAMiSSF101173. SSF101173. 1 hit.
    SSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 4 hits.
    SSF53901. SSF53901. 3 hits.
    SSF55048. SSF55048. 2 hits.
    PROSITEiPS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 2 hits.
    PS00012. PHOSPHOPANTETHEINE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ALM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNEEKLREY LRRALVDLHQ ARERLHEAES GEREPIAIVA MGCRYPGGVQ     50
    DPEGLWKLVA SGGDAIGEFP ADRGWHLDEL YDPDPDQPGT CYTRHGGFLH 100
    DAGEFDAGFF DISPREALAM DPQQRLLLEI SWETVESAGM DPRSLRGSRT 150
    GVFAGLMYEG YDTGAHRAGE GVEGYLGTGN AGSVASGRVA YAFGFEGPAV 200
    TVDTACSSSL VALHLACQSL RQGECDLALA GGVTVMSTPE RFVEFSRQRG 250
    LAPDGRCKSF AAAADGTGWG EGAGLVLLER LSDARRNGHR VLAVVRGSAV 300
    NQDGASNGLT APNGLAQERV IQQVLTSAGL SASDVDAVEA HGTGTRLGDP 350
    IEAQALIAAY GQDRDRDRPL WLGSVKSNIG HTQAAAGVAG VIKMVMAMRH 400
    GELPRTLHVD EPNSHVDWSA GAVRLLTENI RWPGTGTRRA GVSSFGVSGT 450
    NAHVILEHDP LAVTENEEAA QSPAPGIVPW ALSGRSSTAL RAQAERLREL 500
    CEQTDPDPVD VGFSLAATRT AWEHRAVVLG RDSATLRSGL GVVASGEPAV 550
    DVVEGSVLDG EVVFVFPGQG WQWAGMAVDL LDASPTFARH MDECATALRR 600
    YVDWSLVDVL RGAENSPPLD RVDVLQPASF AVMVSLAEVW RSYGVRPAAV 650
    VGHSQGEIAA ACAAGVLPLE DAARLVALRS RALKGLSGRG GMASLACPAD 700
    EVAALFAGSG GRLEVAAING PRSVVVSGDL EAVDELLAEC AEKDMRARRI 750
    PVDYASHSAH VEVVRSPVLA AAAGVRHRDG QVPWWSTVIG DWVDPARLDG 800
    EYWYRNLRQP VRFEHAVQGL VERGFGLFIE MSAHPVLTTA VEETGAESET 850
    AVAAVGTLRR DSGGLRRLLH SLAEAYVRGA TVDWAVAFGG AGRRLDLPTY 900
    PFQRQRYWLD KGAASDEARA VSDPAAGWFW QAVARQDLKS VSDALDLDAD 950
    APLSATLPAL SVWHRQERER VLADGWRYRV DWVRVAPQPV RRTRETWLLV 1000
    VPPGGIEEAL VERLTDALNT RGISTLRLDV PPAATSGELA TELRAAADGD 1050
    PVKAILSLTA LDERPHPECK DVPSGIALLL NLVKALGEAD LRIPLWTITR 1100
    GAVKAGPADR LLRPMQAQAW GLGRVAALEH PERWGGLIDL PDSLDGDVLT 1150
    RLGEALTNGL AEDQLAIRQS GVLARRLVPA PANQPAGRKW RPRGSALITG 1200
    GLGAVGAQVA RWLAEIGAER IVLTSRRGNQ AAGAAELEAE LRALGAQVSI 1250
    VACDVTDRAE MSALLAEFDV TAVFHAAGVG RLLPLAETDQ NGLAEICAAK 1300
    VRGAQVLDEL CDSTDLDAFV LFSSGAGVWG GGGQGAYGAA NAFLDTLAEQ 1350
    RRARGLPATS ISWGSWAGGG MADGAAGEHL RRRGIRPMPA ASAILALQEV 1400
    LDQDETCVSI ADVDWDRFVP TFAATRATRL FDEVPAARKA MPANGPAEPG 1450
    GSPFARNLAE LPEAQRRHEL VDLVCAQVAT VLGHGSREEV QPERAFRALG 1500
    FDSLMAVDLR NRLTTATGLR LPTTTVFDYP NPAALAAHLL EELVGDVASA 1550
    AVTAASAPAS DEPIAIVAMS CRFPGGAHSP EDLWRLVAAG TEVIGEFPSD 1600
    RGWDAEGLYD PDASRPGTTY ARMAGFLYDA GEFDADLFGI SPREALAMDP 1650
    QQRLVLEIAW EALERAGIDP LSLKGSGVGT YIGAGSRGYA TDVRQFPEEA 1700
    EGYLLTGTSA SVLSGRVAYS FGFEGPAVTV DTACSSSLVA LHLACQSLRS 1750
    GECDLALAGG VTVMSTPEMF VEFSRQRGLA PDGRCKSFAE SADGTGWGEG 1800
    AGLLLLERLS DAHRNGHRVL AVVRGSAVNQ DGASNGLAAP NGPSQQRVIN 1850
    QALANAALSA SDVDAVEAHG TGTRLGDPIE AQALIATYGQ ARERDRPLWL 1900
    GSVKSNIGHT QAAAGVAGVI KMVMAMRHGQ LPASLHADEP TSEVDWSSGA 1950
    VRLLAEQVPW PESDRVRRVG VSSFGISGTN AHVILEQATN APDSTAETDK 2000
    TESGSTVDIP VVPWLVSGKT TDSLRGQAER VLSQVESRPE QRSLDVAYSL 2050
    ASGRAALDER AVVLGADRGE LVAGLAALAA GQEASGVISG TRASARFGFV 2100
    FSGQGGQWLG MGRALYSKFP VFAAAFDEAC AELEAHLGED RRVRDVVFGS 2150
    DAQLLDQTLW AQSGLFALQA GLLGLLGSWG VRPDVVMGHS VGELAAAFAA 2200
    GVLSLRDAAR LVAARARLMQ ALPSDGAMLA VAAGEDLVRP LLAGREESVS 2250
    VAALNAPGSV VLSGDREVLA SIVGRLTELR VRTRRLRVSH AFHSHRMDPM 2300
    LGEFAQIAES AEFGKPTTPL VSTLTGELDR AAEMSTPGYW VRQAREPVRF 2350
    ADGVQALAAQ GIGTVVELGP DGTLAALVRE CATESDRVGR ISSIPLMRRE 2400
    RDETRSVMTA LAHLHTRGGE VDWQAFFAGT GARQLELPTY AFQRQHYWIE 2450
    SSARPARDRA DIGEVAEQFW TAVDQGDLAT LVAALDLGAD DDTCASLSDV 2500
    LPALSSWRSG LRNRSLVDSC RYRISWHSSR EVPAPKISGT WLLVVPGAAD 2550
    DGLVTALTSS LVGGGAEVVR IGLSEEDPHR EDVAQRLANA LTDAGQLGGV 2600
    LSLLGLDESP APGFSCLPTG FALTVQLLRA LRKADVEAPF WAVTRGGVAL 2650
    EDVRVSPEQA LVWGLLRVAG LEHPEFWGGL IDLPSDWDDR LGARLAGVLA 2700
    DGGEDQVAIR RGGVFVRRLE RAGASGAGSV WRPRGTVLVT GGTGGLGAHV 2750
    ARWLAGAGAE HVVLTSRRGA DAPGAGELRA ELEALGARVS IVPCDVADRD 2800
    AVAGVLAGIG GECPLTAVVH AAGVGEAGDV VEMGLADFAA VLSAKVRGAA 2850
    NLDELLADSE LDAFVMFSSV SGVWGAGGQG AYAAANAYLD ALAEQRRARG 2900
    LVGTAVAWGP WAGDGMAAGE TGAQLHRMGL ASMEPSAALL ALQGALDRDE 2950
    TSLVVADVDW ARFAPAFTSA RRRPLLDTID EARAALETTG EQAGTGKPVE 3000
    LTQRLAGLSR KERDDAVLDL VRAETAAVLG RDDATALAPS RPFQELGFDS 3050
    LMAVELRNRL NTATGIQLPA STIFDYPNAE SLSRHLCAEL FPTETTVDSA 3100
    LAELDRIEQQ LSMLTGEARA RDRIATRLRA LHEKWNSAAE VPTGADVLST 3150
    LDSATHDEIF EFIDNELDLS 3170
    Length:3,170
    Mass (Da):334,879
    Last modified:June 1, 2001 - v1
    Checksum:i205D519FDFA3249C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY007564 Genomic DNA. Translation: AAG23266.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY007564 Genomic DNA. Translation: AAG23266.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IMP X-ray 2.57 A/B/C/D 913-1471 [» ]
    ProteinModelPortali Q9ALM4.
    SMRi Q9ALM4. Positions 4-910, 3000-3090.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1200.10. 2 hits.
    3.40.366.10. 4 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 2 hits.
    PF00106. adh_short. 2 hits.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 2 hits.
    PF02801. Ketoacyl-synt_C. 2 hits.
    PF00550. PP-binding. 2 hits.
    [Graphical view ]
    SMARTi SM00827. PKS_AT. 2 hits.
    SM00822. PKS_KR. 2 hits.
    SM00825. PKS_KS. 2 hits.
    SM00823. PKS_PP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF101173. SSF101173. 1 hit.
    SSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 4 hits.
    SSF53901. SSF53901. 3 hits.
    SSF55048. SSF55048. 2 hits.
    PROSITEi PS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 2 hits.
    PS00012. PHOSPHOPANTETHEINE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa."
      Waldron C., Matsushima P., Rosteck P.R. Jr, Broughton M.C., Turner J., Madduri K., Crawford K.P., Merlo D.J., Baltz R.H.
      Chem. Biol. 8:487-499(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The missing linker: a dimerization motif located within polyketide synthase modules."
      Zheng J., Fage C.D., Demeler B., Hoffman D.W., Keatinge-Clay A.T.
      ACS Chem. Biol. 8:1263-1270(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) OF 913-1471 IN COMPLEX WITH NADP.

    Entry informationi

    Entry nameiQ9ALM4_9PSEU
    AccessioniPrimary (citable) accession number: Q9ALM4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3