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Q9ALJ4 (Q9ALJ4_GEOSE) Unreviewed, UniProtKB/TrEMBL

Last modified December 11, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names
Gene names
Name:agaA EMBL AAG49420.1
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus) EMBL AAG49420.1
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region144 – 1452Sulfate 2 binding

Sites

Binding site131Sulfate 1; via amide nitrogen and carbonyl oxygen
Binding site531Fructose PDB 4FNU
Binding site1051Sulfate 2
Binding site1391Sulfate 2; via amide nitrogen and carbonyl oxygen
Binding site1391Sulfate 3; via amide nitrogen and carbonyl oxygen PDB 4FNS
Binding site1441Sulfate 3; via amide nitrogen and carbonyl oxygen PDB 4FNS
Binding site2001Sulfate 4; via carbonyl oxygen PDB 4FNS
Binding site2001Sulfate 5; via carbonyl oxygen PDB 4FNS
Binding site3401Glucose PDB 4FNU
Binding site5491Sulfate 4
Binding site5491Sulfate 5
Binding site5541Sulfate 4
Binding site5541Sulfate 5
Binding site6721Sulfate 2; via amide nitrogen PDB 4FNS
Binding site6721Sulfate 4; via amide nitrogen
Binding site6721Sulfate 5; via amide nitrogen
Binding site6721Sulfate 6; via amide nitrogen PDB 4FNS

Sequences

Sequence LengthMass (Da)Tools
Q9ALJ4 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 6E59129E1C3502A2

FASTA72983,211
        10         20         30         40         50         60 
MSVAYNPQTK QFHLRAGKAS YVMQLFRSGY LAHVYWGKAV RDVRGARAFP RLDRAFSPNP 

        70         80         90        100        110        120 
DPSDRTFSLD TLLQEYPAYG NTDFRAPAYQ VQLENGSTVT DLRYKTHRIY KGKPRLNGLP 

       130        140        150        160        170        180 
ATYVEHEQEA ETLEIVLGDA LIGLEVTLQY TAYEKWNVIT RSARFENKGG ERLKLLRALS 

       190        200        210        220        230        240 
MSVDFPTADY DWIHLPGAWG RERWIERRPL VTGVQAAESR RGASSHQQNP FIALVAKNAD 

       250        260        270        280        290        300 
EHQGEVYGFS FVYSGNFLAQ IEVDQFGTAR VSMGINPFDF TWLLQPGESF QTPEVVMVYS 

       310        320        330        340        350        360 
DQGLNGMSQT YHELYRTRLA RGAFRDRERP ILINNWEATY FDFNEEKIVN IARTAAELGI 

       370        380        390        400        410        420 
ELVVLDDGWF GERDDDRRSL GDWIVNRRKL PNGLDGLAKQ VNELGLQFGL WVEPEMVSPN 

       430        440        450        460        470        480 
SELYRKHPDW CLHVPNRPRS EGRNQLVLDY SREDVCDYII ETISNVLASA PITYVKWDMN 

       490        500        510        520        530        540 
RHMTEIGSSA LPPERQRETA HRYMLGLYRV MDEITSRFPH ILFESCSGGG GRFDPGMLYY 

       550        560        570        580        590        600 
MPQTWTSDNT DAVSRLKIQY GTSLVYPISA MGAHVSAVPN HQVGRVASLK TRGHVAMSGN 

       610        620        630        640        650        660 
FGYELDITKL TETEKQMMKQ QVAFYKDVRR LVQFGTFYRL LSPFEGNEAA WMFVSADRSE 

       670        680        690        700        710        720 
ALVAYFRVLA EANAPLSYLR LKGLDSNQDY EIEGLGVYGG DELMYAGVAL PYRSSDFISM 


MWRLKAVQQ 

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References

[1]"Production of thermostable, recombinant alpha-galactosidase suitable for raffinose elimination from sugar beet syrup."
Ganter C., Boeck A., Buckel P., Mattes R.
J. Biotechnol. 8:301-310(1988)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: KVE39 EMBL AAG49420.1.
[2]Watzlawick H., Ganter C., Mattes R.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: KVE39 EMBL AAG49420.1.
[3]"The molecular mechanism of thermostable ?-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies."
Merceron R., Foucault M., Haser R., Mattes R., Watzlawick H., Gouet P.
J. Biol. Chem. 287:39642-39652(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH FRUCTOSE; GLUCOSE AND SULFATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY013286 Genomic DNA. Translation: AAG49420.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4FNPX-ray2.80A/B/C/D1-729[»]
4FNRX-ray3.20A/B/C/D1-729[»]
4FNSX-ray2.60A/B/C/D1-729[»]
4FNTX-ray2.60A/B/C/D1-729[»]
4FNUX-ray3.60A/B/C/D1-729[»]
ProteinModelPortalQ9ALJ4.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFPIRSF005536. Agal. 1 hit.
PRINTSPR00743. GLHYDRLASE36.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ9ALJ4_GEOSE
AccessionPrimary (citable) accession number: Q9ALJ4
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: December 11, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)