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Protein
Submitted name:

Alpha-galactosidase AgaA

Gene

agaA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531FructoseCombined sources
Binding sitei340 – 3401GlucoseCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-galactosidase AgaAImported
Gene namesi
Name:agaAImported
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FNPX-ray2.80A/B/C/D1-729[»]
4FNRX-ray3.20A/B/C/D1-729[»]
4FNSX-ray2.60A/B/C/D1-729[»]
4FNTX-ray2.60A/B/C/D1-729[»]
4FNUX-ray3.60A/B/C/D1-729[»]
ProteinModelPortaliQ9ALJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ALJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAYNPQTK QFHLRAGKAS YVMQLFRSGY LAHVYWGKAV RDVRGARAFP
60 70 80 90 100
RLDRAFSPNP DPSDRTFSLD TLLQEYPAYG NTDFRAPAYQ VQLENGSTVT
110 120 130 140 150
DLRYKTHRIY KGKPRLNGLP ATYVEHEQEA ETLEIVLGDA LIGLEVTLQY
160 170 180 190 200
TAYEKWNVIT RSARFENKGG ERLKLLRALS MSVDFPTADY DWIHLPGAWG
210 220 230 240 250
RERWIERRPL VTGVQAAESR RGASSHQQNP FIALVAKNAD EHQGEVYGFS
260 270 280 290 300
FVYSGNFLAQ IEVDQFGTAR VSMGINPFDF TWLLQPGESF QTPEVVMVYS
310 320 330 340 350
DQGLNGMSQT YHELYRTRLA RGAFRDRERP ILINNWEATY FDFNEEKIVN
360 370 380 390 400
IARTAAELGI ELVVLDDGWF GERDDDRRSL GDWIVNRRKL PNGLDGLAKQ
410 420 430 440 450
VNELGLQFGL WVEPEMVSPN SELYRKHPDW CLHVPNRPRS EGRNQLVLDY
460 470 480 490 500
SREDVCDYII ETISNVLASA PITYVKWDMN RHMTEIGSSA LPPERQRETA
510 520 530 540 550
HRYMLGLYRV MDEITSRFPH ILFESCSGGG GRFDPGMLYY MPQTWTSDNT
560 570 580 590 600
DAVSRLKIQY GTSLVYPISA MGAHVSAVPN HQVGRVASLK TRGHVAMSGN
610 620 630 640 650
FGYELDITKL TETEKQMMKQ QVAFYKDVRR LVQFGTFYRL LSPFEGNEAA
660 670 680 690 700
WMFVSADRSE ALVAYFRVLA EANAPLSYLR LKGLDSNQDY EIEGLGVYGG
710 720
DELMYAGVAL PYRSSDFISM MWRLKAVQQ
Length:729
Mass (Da):83,211
Last modified:June 1, 2001 - v1
Checksum:i6E59129E1C3502A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY013286 Genomic DNA. Translation: AAG49420.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY013286 Genomic DNA. Translation: AAG49420.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FNPX-ray2.80A/B/C/D1-729[»]
4FNRX-ray3.20A/B/C/D1-729[»]
4FNSX-ray2.60A/B/C/D1-729[»]
4FNTX-ray2.60A/B/C/D1-729[»]
4FNUX-ray3.60A/B/C/D1-729[»]
ProteinModelPortaliQ9ALJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Production of thermostable, recombinant alpha-galactosidase suitable for raffinose elimination from sugar beet syrup."
    Ganter C., Boeck A., Buckel P., Mattes R.
    J. Biotechnol. 8:301-310(1988)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KVE39Imported.
  2. Watzlawick H., Ganter C., Mattes R.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KVE39Imported.
  3. "The molecular mechanism of thermostable alpha-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies."
    Merceron R., Foucault M., Haser R., Mattes R., Watzlawick H., Gouet P.
    J. Biol. Chem. 287:39642-39652(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH FRUCTOSE AND GLUCOSE.

Entry informationi

Entry nameiQ9ALJ4_GEOSE
AccessioniPrimary (citable) accession number: Q9ALJ4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.