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Q9ALJ4

- Q9ALJ4_GEOSE

UniProt

Q9ALJ4 - Q9ALJ4_GEOSE

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Protein
Submitted name: Alpha-galactosidase AgaA
Gene
agaA
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Sulfate 1; via amide nitrogen and carbonyl oxygen
Binding sitei53 – 531FructoseImported
Binding sitei105 – 1051Sulfate 2
Binding sitei139 – 1391Sulfate 2; via amide nitrogen and carbonyl oxygen
Binding sitei340 – 3401GlucoseImported
Binding sitei549 – 5491Sulfate 4
Binding sitei549 – 5491Sulfate 5
Binding sitei554 – 5541Sulfate 4
Binding sitei554 – 5541Sulfate 5
Binding sitei672 – 6721Sulfate 4; via amide nitrogen
Binding sitei672 – 6721Sulfate 5; via amide nitrogen

GO - Molecular functioni

  1. alpha-galactosidase activity Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-galactosidase AgaAImported
Gene namesi
Name:agaAImported
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FNPX-ray2.80A/B/C/D1-729[»]
4FNRX-ray3.20A/B/C/D1-729[»]
4FNSX-ray2.60A/B/C/D1-729[»]
4FNTX-ray2.60A/B/C/D1-729[»]
4FNUX-ray3.60A/B/C/D1-729[»]
ProteinModelPortaliQ9ALJ4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1452Sulfate 2 binding

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ALJ4-1 [UniParc]FASTAAdd to Basket

« Hide

MSVAYNPQTK QFHLRAGKAS YVMQLFRSGY LAHVYWGKAV RDVRGARAFP    50
RLDRAFSPNP DPSDRTFSLD TLLQEYPAYG NTDFRAPAYQ VQLENGSTVT 100
DLRYKTHRIY KGKPRLNGLP ATYVEHEQEA ETLEIVLGDA LIGLEVTLQY 150
TAYEKWNVIT RSARFENKGG ERLKLLRALS MSVDFPTADY DWIHLPGAWG 200
RERWIERRPL VTGVQAAESR RGASSHQQNP FIALVAKNAD EHQGEVYGFS 250
FVYSGNFLAQ IEVDQFGTAR VSMGINPFDF TWLLQPGESF QTPEVVMVYS 300
DQGLNGMSQT YHELYRTRLA RGAFRDRERP ILINNWEATY FDFNEEKIVN 350
IARTAAELGI ELVVLDDGWF GERDDDRRSL GDWIVNRRKL PNGLDGLAKQ 400
VNELGLQFGL WVEPEMVSPN SELYRKHPDW CLHVPNRPRS EGRNQLVLDY 450
SREDVCDYII ETISNVLASA PITYVKWDMN RHMTEIGSSA LPPERQRETA 500
HRYMLGLYRV MDEITSRFPH ILFESCSGGG GRFDPGMLYY MPQTWTSDNT 550
DAVSRLKIQY GTSLVYPISA MGAHVSAVPN HQVGRVASLK TRGHVAMSGN 600
FGYELDITKL TETEKQMMKQ QVAFYKDVRR LVQFGTFYRL LSPFEGNEAA 650
WMFVSADRSE ALVAYFRVLA EANAPLSYLR LKGLDSNQDY EIEGLGVYGG 700
DELMYAGVAL PYRSSDFISM MWRLKAVQQ 729
Length:729
Mass (Da):83,211
Last modified:June 1, 2001 - v1
Checksum:i6E59129E1C3502A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY013286 Genomic DNA. Translation: AAG49420.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY013286 Genomic DNA. Translation: AAG49420.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4FNP X-ray 2.80 A/B/C/D 1-729 [» ]
4FNR X-ray 3.20 A/B/C/D 1-729 [» ]
4FNS X-ray 2.60 A/B/C/D 1-729 [» ]
4FNT X-ray 2.60 A/B/C/D 1-729 [» ]
4FNU X-ray 3.60 A/B/C/D 1-729 [» ]
ProteinModelPortali Q9ALJ4.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02065. Melibiase. 1 hit.
[Graphical view ]
PIRSFi PIRSF005536. Agal. 1 hit.
PRINTSi PR00743. GLHYDRLASE36.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Production of thermostable, recombinant alpha-galactosidase suitable for raffinose elimination from sugar beet syrup."
    Ganter C., Boeck A., Buckel P., Mattes R.
    J. Biotechnol. 8:301-310(1988)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KVE39Imported.
  2. Watzlawick H., Ganter C., Mattes R.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KVE39Imported.
  3. "The molecular mechanism of thermostable ?-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies."
    Merceron R., Foucault M., Haser R., Mattes R., Watzlawick H., Gouet P.
    J. Biol. Chem. 287:39642-39652(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH FRUCTOSE AND GLUCOSE.

Entry informationi

Entry nameiQ9ALJ4_GEOSE
AccessioniPrimary (citable) accession number: Q9ALJ4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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