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Q9ALJ4

- Q9ALJ4_GEOSE

UniProt

Q9ALJ4 - Q9ALJ4_GEOSE

Protein
Submitted name:

Alpha-galactosidase AgaA

Gene

agaA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131Sulfate 1; via amide nitrogen and carbonyl oxygen
    Binding sitei53 – 531FructoseImported
    Binding sitei105 – 1051Sulfate 2
    Binding sitei139 – 1391Sulfate 2; via amide nitrogen and carbonyl oxygen
    Binding sitei340 – 3401GlucoseImported
    Binding sitei549 – 5491Sulfate 4
    Binding sitei549 – 5491Sulfate 5
    Binding sitei554 – 5541Sulfate 4
    Binding sitei554 – 5541Sulfate 5
    Binding sitei672 – 6721Sulfate 4; via amide nitrogen
    Binding sitei672 – 6721Sulfate 5; via amide nitrogen

    GO - Molecular functioni

    1. alpha-galactosidase activity Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Alpha-galactosidase AgaAImported
    Gene namesi
    Name:agaAImported
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4FNPX-ray2.80A/B/C/D1-729[»]
    4FNRX-ray3.20A/B/C/D1-729[»]
    4FNSX-ray2.60A/B/C/D1-729[»]
    4FNTX-ray2.60A/B/C/D1-729[»]
    4FNUX-ray3.60A/B/C/D1-729[»]
    ProteinModelPortaliQ9ALJ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni144 – 1452Sulfate 2 binding

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002252. Glyco_hydro_36.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005536. Agal. 1 hit.
    PRINTSiPR00743. GLHYDRLASE36.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ALJ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVAYNPQTK QFHLRAGKAS YVMQLFRSGY LAHVYWGKAV RDVRGARAFP    50
    RLDRAFSPNP DPSDRTFSLD TLLQEYPAYG NTDFRAPAYQ VQLENGSTVT 100
    DLRYKTHRIY KGKPRLNGLP ATYVEHEQEA ETLEIVLGDA LIGLEVTLQY 150
    TAYEKWNVIT RSARFENKGG ERLKLLRALS MSVDFPTADY DWIHLPGAWG 200
    RERWIERRPL VTGVQAAESR RGASSHQQNP FIALVAKNAD EHQGEVYGFS 250
    FVYSGNFLAQ IEVDQFGTAR VSMGINPFDF TWLLQPGESF QTPEVVMVYS 300
    DQGLNGMSQT YHELYRTRLA RGAFRDRERP ILINNWEATY FDFNEEKIVN 350
    IARTAAELGI ELVVLDDGWF GERDDDRRSL GDWIVNRRKL PNGLDGLAKQ 400
    VNELGLQFGL WVEPEMVSPN SELYRKHPDW CLHVPNRPRS EGRNQLVLDY 450
    SREDVCDYII ETISNVLASA PITYVKWDMN RHMTEIGSSA LPPERQRETA 500
    HRYMLGLYRV MDEITSRFPH ILFESCSGGG GRFDPGMLYY MPQTWTSDNT 550
    DAVSRLKIQY GTSLVYPISA MGAHVSAVPN HQVGRVASLK TRGHVAMSGN 600
    FGYELDITKL TETEKQMMKQ QVAFYKDVRR LVQFGTFYRL LSPFEGNEAA 650
    WMFVSADRSE ALVAYFRVLA EANAPLSYLR LKGLDSNQDY EIEGLGVYGG 700
    DELMYAGVAL PYRSSDFISM MWRLKAVQQ 729
    Length:729
    Mass (Da):83,211
    Last modified:June 1, 2001 - v1
    Checksum:i6E59129E1C3502A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY013286 Genomic DNA. Translation: AAG49420.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY013286 Genomic DNA. Translation: AAG49420.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4FNP X-ray 2.80 A/B/C/D 1-729 [» ]
    4FNR X-ray 3.20 A/B/C/D 1-729 [» ]
    4FNS X-ray 2.60 A/B/C/D 1-729 [» ]
    4FNT X-ray 2.60 A/B/C/D 1-729 [» ]
    4FNU X-ray 3.60 A/B/C/D 1-729 [» ]
    ProteinModelPortali Q9ALJ4.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002252. Glyco_hydro_36.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005536. Agal. 1 hit.
    PRINTSi PR00743. GLHYDRLASE36.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Production of thermostable, recombinant alpha-galactosidase suitable for raffinose elimination from sugar beet syrup."
      Ganter C., Boeck A., Buckel P., Mattes R.
      J. Biotechnol. 8:301-310(1988)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: KVE39Imported.
    2. Watzlawick H., Ganter C., Mattes R.
      Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: KVE39Imported.
    3. "The molecular mechanism of thermostable ?-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies."
      Merceron R., Foucault M., Haser R., Mattes R., Watzlawick H., Gouet P.
      J. Biol. Chem. 287:39642-39652(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH FRUCTOSE AND GLUCOSE.

    Entry informationi

    Entry nameiQ9ALJ4_GEOSE
    AccessioniPrimary (citable) accession number: Q9ALJ4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3