Reviewed, UniProtKB/Swiss-Prot Q9AKP1 (MURF_RICMO)

Names and origin

Protein names

Recommended name:
    Probable UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
    EC=6.3.2.10
Alternative name(s):
    UDP-MurNAc-pentapeptide synthetase
    D-alanyl-D-alanine-adding enzyme

Gene names

Name:

murF

Organism

Rickettsia montana

Taxonomic identifier

33991 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Probable UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase		PRO_0000259657
Regions
	Nucleotide binding	106 – 112	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9AKP1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 59FDCF74127534A0	FASTA	449	50,322
	10 20 30 40 50 60 MIWNSKTLSA ALGITISNSI NCNEVQFNSK DVKKGDLFIA LQGNRDGHDY VLDAIDKGAA 70 80 90 100 110 120 AVIISKQVEI NDKDKIILVD DCFEALQKMA LYKRENSKAK FIVITGSVGK TSTKEALKVL 130 140 150 160 170 180 LQHDFLVFAS RGNFNNKLGM LINLASMADD TEYAIFELGM NHKGEIRELV QILKPNIAMI 190 200 210 220 230 240 TNISEAHLEF FNSLEEIAEA KCEIFANFSK NDIAVINADT NCYNKILSIL KNLSITDIHS 250 260 270 280 290 300 FGRSSKTSAE LILYENLGEQ VYLKYKINNK VLDVTIPFIP RHFTENYTGV LLIIDILGKD 310 320 330 340 350 360 IEIAANHLAN ISPTKGRGEI INIQNCRVIC DYYNASPQSM KAALEYLKQV PAENKTAIIG 370 380 390 400 410 420 DMLELGENSK RLHEKLVQYI LDAACSKVYL VGVNTKYIDD LLPSKIAKKY FKNVDELMTH 430 440 ITDLFEGNEL ILIKGSRGVK LDKIVDYYK

References

[1]	"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes." Andersson J.O., Andersson S.G.E. Mol. Biol. Evol. 18:829-839(2001) [PubMed: 11319266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ293315 Genomic DNA. Translation: CAC33607.1.
3D structure databases
HSSP	HSSP built from PDB template 1GG4 based on UniProtKB P11880.
ModBase	Search...
Family and domain databases
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-3pep_AlaAla_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PANTHER	PTHR23135:SF3. MurF. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURF_RICMO

Accession

Primary (citable) accession number: Q9AKP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)