Q9AJS0 (Q9AJS0_ALIAC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cellulase EMBL CAC34051.1
EC=3.2.1.4 EMBL CAC34051.1

Gene names

Name:

celA EMBL CAC34051.1

Organism

Alicyclobacillus acidocaldarius (Bacillus acidocaldarius) EMBL CAC34051.1

Taxonomic identifier

1388 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Alicyclobacillaceae › Alicyclobacillus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 3EZ8 PDB 3H2W PDB 3H3K Metal-binding PDB 3EZ8 PDB 3H2W PDB 3H3K Zinc PDB 3EZ8 PDB 3H2W PDB 3H3K
Molecular function	Glycosidase EMBL CAC34051.1 Hydrolase
Technical term	3D-structure PDB 3EZ8 PDB 3H2W PDB 3H3K
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	143 – 146	4	Glucose binding PDB 3H2W PDB 3H3K
	Region	298 – 300	3	Glucose binding PDB 3H2W PDB 3H3K
Sites
	Metal binding	104	1	Zinc PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	121	1	Zinc PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	122	1	Zinc; via pros nitrogen PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	142	1	Zinc; via tele nitrogen PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	302	1	Calcium PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	304	1	Calcium; via carbonyl oxygen PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	307	1	Calcium PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	308	1	Calcium PDB 3EZ8 PDB 3H2W PDB 3H3K
	Metal binding	344	1	Calcium; via carbonyl oxygen PDB 3EZ8 PDB 3H2W PDB 3H3K
	Binding site	401	1	Glucose PDB 3H2W PDB 3H3K
	Binding site	515	1	Glucose PDB 3H2W PDB 3H3K
	Binding site	519	1	Glucose PDB 3H2W PDB 3H3K

Sequences

	Sequence		Length	Mass (Da)
	Q9AJS0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 19A6C441120EFAD4	FASTA	537	58,963
	10 20 30 40 50 60 MPSRVPKSIF YNQVGYLISG DKRFWIQAHE PQPFALRTPE GQAVFAGMTK PVGGNWYVGD 70 80 90 100 110 120 FTALRVPGTY TLTVGTLEAR VVIHRRAYRD VLEAMLRFFD YQLCGVVLPE DEAGPWAHGA 130 140 150 160 170 180 CHTSDAKVFG TERALACPGG WHDAGDYGKY TVPAAKAVAD LLLAHEYFPA ALAHVRPMRS 190 200 210 220 230 240 VHRAPHLPPA LEVAREEIAW LLTMQDPATG GVYHKVTTPS FPPLDTRPED DDAPLVLSPI 250 260 270 280 290 300 SYAATATFCA AMAHAALVYR PFDPALSSCC ADAARRAYAW LGAHEMQPFH NPDGILTGEY 310 320 330 340 350 360 GDAELRDELL WASCALLRMT GDSAWARVCE PLLDLDLPWE LGWADVALYG VMDYLRTPRA 370 380 390 400 410 420 AVSDDVRNKV KSRLLRELDA LAAMAESHPF GIPMRDDDFI WGSNMVLLNR AMAFLLAEGV 430 440 450 460 470 480 GVLHPAAHTV AQRAADYLFG ANPLGQCYVT GFGQRPVRHP HHRPSVADDV DHPVPGMVVG 490 500 510 520 530 GPNRHLQDEI ARAQLAGRPA MEAYIDHQDS YSTNEVAVYW NSPAVFVIAA LLEARGR

References

[1]	"Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009." Eckert K., Zielinski F., Lo Leggio L., Schneider E. Appl. Microbiol. Biotechnol. 60:428-436(2002) [PubMed: 12466883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC27009 EMBL CAC34051.1.
[2]	Eckert K.J. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC27009 EMBL CAC34051.1.
[3]	"Structure of endoglucanase Cel9A from the thermoacidophilic Alicyclobacillus acidocaldarius." Pereira J.H., Sapra R., Volponi J.V., Kozina C.L., Simmons B., Adams P.D. Acta Crystallogr. D Biol. Crystallogr. 65:744-750(2009) [PubMed: 19622857] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND ZINC.
[4]	"Crystal structures of A. acidocaldarius endoglucanase Cel9A in complex with cello-oligosaccharides: strong -1 and -2 subsites mimic cellobiohydrolase activity." Eckert K., Vigouroux A., Lo Leggio L., Morera S. J. Mol. Biol. 394:61-70(2009) [PubMed: 19729024] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH CALCIUM; GLUCOSE AND ZINC.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ308623 Genomic DNA. Translation: CAC34051.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EZ8	X-ray	2.30	A	1-537	[»]
3H2W	X-ray	2.66	A	1-537	[»]
3H3K	X-ray	2.10	A	1-537	[»]
3RX5	X-ray	1.99	A	1-537	[»]
3RX7	X-ray	2.02	A	1-537	[»]
3RX8	X-ray	2.56	A	1-537	[»]

ProteinModelPortal

Q9AJS0.

ModBase

Search...

Protein family/group databases

CAZy

GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]

Gene3D

G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF02927. CelD_N. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]

SUPFAM

SSF48208. Glyco_trans_6hp. 1 hit.
SSF81296. Ig_E-set. 1 hit.

PROSITE

PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9AJS0_ALIAC

Accession

Primary (citable) accession number: Q9AJS0

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	October 19, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)