Reviewed, UniProtKB/Swiss-Prot Q9AJN6 (TREZ_ARTRM)

Names and origin

Protein names

Recommended name:
    Malto-oligosyltrehalose trehalohydrolase
      Short name=MTHase
    EC=3.2.1.141
Alternative name(s):
    4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
    Maltooligosyl trehalose trehalohydrolase

Gene names

Name:

treZ

Organism

Arthrobacter ramosus

Taxonomic identifier

1672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Malto-oligosyltrehalose trehalohydrolase		PRO_0000054322
Sites
	Active site	250	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AJN6-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 284A3F20207E228B	FASTA	575	63,080
	10 20 30 40 50 60 MNRRFPVWAP QAAQVTLVVG QGRAELPLTR DENGWWALQQ PWDGGPDLVD YGYLVDGKGP 70 80 90 100 110 120 FADPRSLRQP RGVHELGREF DPARYAWGDD GWRGRDLTGA VIYELHVGTF TPEGTLDSAI 130 140 150 160 170 180 RRLDHLVRLG VDAVELLPVN AFNGTHGWGY DGVLWYAVHE PYGGPEAYQR FVDACHARGL 190 200 210 220 230 240 AVVQDVVYNH LGPSGNHLPD FGPYLGSGAA NTWGDALNLD GPLSDEVRRY IIDNAVYWLR 250 260 270 280 290 300 DMHADGLRLD AVHALRDARA LHLLEELAAR VDELAGELGR PLTLIAESDL NDPKLIRSRA 310 320 330 340 350 360 AHGYGLDAQW DDDVHHAVHA NVTGETVGYY ADFGGLGALV KVFQRGWFHD GTWSSFRERH 370 380 390 400 410 420 HGRPLDPDIP FRRLVAFAQD HDQVGNRAVG DRMSAQVGEG SLAAAAALVL LGPFTPMLFM 430 440 450 460 470 480 GEEWGARTPW QFFTSHPEPE LGEATARGRI AEFARMGWDP AVVPDPQDPA TFARSHLDWS 490 500 510 520 530 540 EPEREPHAGL LAFYTDLIAL RRELPVDAPA REVDADEARG VFAFSRGPLR VTVALRPGPV 550 560 570 GVPEHGGLVL AYGEVRAGAA GLHLDGPGAA IVRLE

References

[1]	"Trehalose-producing operon treYZ from Arthrobacter ramosus S34." Yamamoto T., Maruta K., Watanabe H., Yamashita H., Kubota M., Fukuda S., Kurimoto M. Biosci. Biotechnol. Biochem. 65:1419-1423(2001) [PubMed: 11471747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S34.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB045141 Genomic DNA. Translation: BAB40766.1.
PIR	JC7727.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	3.2.1.141. 304210.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
TIGRFAMs	TIGR02402. trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TREZ_ARTRM

Accession

Primary (citable) accession number: Q9AJN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)