Q9AJN6 (TREZ_ARTRM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase
Short name=MTHase
EC=3.2.1.141

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase

Gene names

Name:

treZ

Organism

Arthrobacter ramosus

Taxonomic identifier

1672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Malto-oligosyltrehalose trehalohydrolase		PRO_0000054322
Regions
	Region	312 – 316	5	Substrate binding By similarity
Sites
	Active site	250	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
	Site	382	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AJN6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 284A3F20207E228B	FASTA	575	63,080
	10 20 30 40 50 60 MNRRFPVWAP QAAQVTLVVG QGRAELPLTR DENGWWALQQ PWDGGPDLVD YGYLVDGKGP 70 80 90 100 110 120 FADPRSLRQP RGVHELGREF DPARYAWGDD GWRGRDLTGA VIYELHVGTF TPEGTLDSAI 130 140 150 160 170 180 RRLDHLVRLG VDAVELLPVN AFNGTHGWGY DGVLWYAVHE PYGGPEAYQR FVDACHARGL 190 200 210 220 230 240 AVVQDVVYNH LGPSGNHLPD FGPYLGSGAA NTWGDALNLD GPLSDEVRRY IIDNAVYWLR 250 260 270 280 290 300 DMHADGLRLD AVHALRDARA LHLLEELAAR VDELAGELGR PLTLIAESDL NDPKLIRSRA 310 320 330 340 350 360 AHGYGLDAQW DDDVHHAVHA NVTGETVGYY ADFGGLGALV KVFQRGWFHD GTWSSFRERH 370 380 390 400 410 420 HGRPLDPDIP FRRLVAFAQD HDQVGNRAVG DRMSAQVGEG SLAAAAALVL LGPFTPMLFM 430 440 450 460 470 480 GEEWGARTPW QFFTSHPEPE LGEATARGRI AEFARMGWDP AVVPDPQDPA TFARSHLDWS 490 500 510 520 530 540 EPEREPHAGL LAFYTDLIAL RRELPVDAPA REVDADEARG VFAFSRGPLR VTVALRPGPV 550 560 570 GVPEHGGLVL AYGEVRAGAA GLHLDGPGAA IVRLE

References

[1]	"Trehalose-producing operon treYZ from Arthrobacter ramosus S34." Yamamoto T., Maruta K., Watanabe H., Yamashita H., Kubota M., Fukuda S., Kurimoto M. Biosci. Biotechnol. Biochem. 65:1419-1423(2001) [PubMed: 11471747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB045141 Genomic DNA. Translation: BAB40766.1.
PIR	JC7727.
3D structure databases
ProteinModelPortal	Q9AJN6.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TREZ_ARTRM

Accession

Primary (citable) accession number: Q9AJN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program