Q9AJD6 (PNO_MICLT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxine 4-oxidase
EC=1.1.3.12

Gene names

Name:

pno

Organism

Microbacterium luteolum (Aureobacterium luteolum)

Taxonomic identifier

69367 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Microbacterium

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Pyridoxine + O2 = pyridoxal + H2O2.
Cofactor	FAD.
Pathway	Cofactor degradation; B6 vitamer degradation; pyridoxal from pyridoxine (oxidase route): step 1/1.
Subunit structure	Monomer.
Sequence similarities	Belongs to the GMC oxidoreductase family.
Biophysicochemical properties	Kinetic parameters: KM=54.5 µM for pyridoxine Ref.1 KM=206.64 µM for oxygen KM=9.71 µM for 2,6-dichloroindophenol KM=50.16 µM for vitamin K3 pH dependence: Optimum pH is 7.5-8.0. Temperature dependence: Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alcohol metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	choline dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro pyridoxine 4-oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 507	506	Pyridoxine 4-oxidase		PRO_0000205614
Sites
	Active site	448	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AJD6 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 64B3A9A094E0AE21	FASTA	507	54,196
	10 20 30 40 50 60 MAQYDVAIIG AGSAGALIAA RLSEDPARNV LLIEAGGRPS DPDILKPSMW PAIQHRSYDW 70 80 90 100 110 120 DYKTTPQEGA AGRSFAWARG KGLGGSSLLH AMGYMRGHPA DFAAWAEATG DERWSWEGLL 130 140 150 160 170 180 PSFMANEDHV SGGDGIHGKD GPMPVWIPDD EVSPLTQAFM TAGNALGLPR IPDHNTGQMI 190 200 210 220 230 240 GVTPNSLMIR DGRRVTVAEA WLTPEVCARP NLTIMTGTLT RRLKLEKSHV SAIELAGPEG 250 260 270 280 290 300 LATVTASEII LSAGSLESPA LLMRSGIGRE NVLREAGVTC RVKAPELGLN LMDHLLGAGN 310 320 330 340 350 360 LYATKKHLPP SRLQHSESMA YMRAGDFSAG GQPEIVVGCG VAPIVSESFT APAPGNAYSF 370 380 390 400 410 420 LFGVTHPTSR GEIRITGDAP DSPLIIDPRY LQTQNDRNLF RAALGAAREI GHRPELAEWR 430 440 450 460 470 480 DHEILPKSLA ASQDIDTFIA KAVITHHHPS GTCRMGKDEM SVVDADLRLR GLDNLYVVDG 490 500 SVLPSLTAGP IHAAVQAIAE NFTTGFK

References

[1]	"Purification, molecular cloning, and characterization of pyridoxine 4-oxidase from Microbacterium." Kaneda Y., Ohnishi K., Yagi T. Biosci. Biotechnol. Biochem. 66:1022-1031(2002) [PubMed: 12092811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20 AND 348-364, CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: YK-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB049341 Genomic DNA. Translation: BAB39853.1.
PIR	JC7855.
3D structure databases
ProteinModelPortal	Q9AJD6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNO_MICLT

Accession

Primary (citable) accession number: Q9AJD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 52 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program