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Protein

HTH-type transcriptional regulator TtgR

Gene

ttgR

Organism
Pseudomonas putida (strain DOT-T1E)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi33 – 52H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator TtgR
Alternative name(s):
Toluene efflux pump ttgABC operon repressor
Gene namesi
Name:ttgR
Ordered Locus Names:T1E_0244
OrganismiPseudomonas putida (strain DOT-T1E)
Taxonomic identifieri1196325 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006503 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000706231 – 210HTH-type transcriptional regulator TtgRAdd BLAST210

Expressioni

Inductioni

Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 27Combined sources21
Turni28 – 31Combined sources4
Helixi34 – 41Combined sources8
Helixi47 – 51Combined sources5
Helixi55 – 68Combined sources14
Helixi71 – 77Combined sources7
Helixi85 – 102Combined sources18
Helixi104 – 115Combined sources12
Turni122 – 124Combined sources3
Helixi125 – 150Combined sources26
Helixi160 – 180Combined sources21
Helixi182 – 184Combined sources3
Turni187 – 190Combined sources4
Helixi191 – 204Combined sources14
Helixi206 – 208Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UXHX-ray2.40A/B1-210[»]
2UXIX-ray2.50A/B1-210[»]
2UXOX-ray2.70A/B1-210[»]
2UXPX-ray2.70A/B1-210[»]
2UXUX-ray2.30A/B1-210[»]
2XDNX-ray2.20A/B/C/D1-210[»]
ProteinModelPortaliQ9AIU0.
SMRiQ9AIU0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AIU0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 70HTH tetR-typePROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK03577.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]
PfamiPF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AIU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW
60 70 80 90 100
HFNNKAELVQ ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL
110 120 130 140 150
VLDARTRRIN EILHHKCEFT DDMCEIRQQR QSAVLDCHKG ITLALANAVR
160 170 180 190 200
RGQLPGELDA ERAAVAMFAY VDGLIRRWLL LPDSVDLLGD VEKWVDTGLD
210
MLRLSPALRK
Length:210
Mass (Da):23,854
Last modified:June 1, 2001 - v1
Checksum:i237211F9046B9F9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1.
CP003734 Genomic DNA. Translation: AFO46103.1.

Genome annotation databases

EnsemblBacteriaiAFO46103; AFO46103; T1E_0244.
KEGGippx:T1E_0244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1.
CP003734 Genomic DNA. Translation: AFO46103.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UXHX-ray2.40A/B1-210[»]
2UXIX-ray2.50A/B1-210[»]
2UXOX-ray2.70A/B1-210[»]
2UXPX-ray2.70A/B1-210[»]
2UXUX-ray2.30A/B1-210[»]
2XDNX-ray2.20A/B/C/D1-210[»]
ProteinModelPortaliQ9AIU0.
SMRiQ9AIU0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFO46103; AFO46103; T1E_0244.
KEGGippx:T1E_0244.

Phylogenomic databases

KOiK03577.

Miscellaneous databases

EvolutionaryTraceiQ9AIU0.
PROiQ9AIU0.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]
PfamiPF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTTGR_PSEPT
AccessioniPrimary (citable) accession number: Q9AIU0
Secondary accession number(s): I7B485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.