SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9AIU0

- TTGR_PSEPT

UniProt

Q9AIU0 - TTGR_PSEPT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
HTH-type transcriptional regulator TtgR
Gene
ttgR, T1E_0244
Organism
Pseudomonas putida (strain DOT-T1E)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 5220H-T-H motif Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator TtgR
Alternative name(s):
Toluene efflux pump ttgABC operon repressor
Gene namesi
Name:ttgR
Ordered Locus Names:T1E_0244
OrganismiPseudomonas putida (strain DOT-T1E)
Taxonomic identifieri1196325 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006503: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210HTH-type transcriptional regulator TtgR
PRO_0000070623Add
BLAST

Expressioni

Inductioni

Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline.1 Publication

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2721
Turni28 – 314
Helixi34 – 418
Helixi47 – 515
Helixi55 – 6814
Helixi71 – 777
Helixi85 – 10218
Helixi104 – 11512
Turni122 – 1243
Helixi125 – 15026
Helixi160 – 18021
Helixi182 – 1843
Turni187 – 1904
Helixi191 – 20414
Helixi206 – 2083

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UXHX-ray2.40A/B1-210[»]
2UXIX-ray2.50A/B1-210[»]
2UXOX-ray2.70A/B1-210[»]
2UXPX-ray2.70A/B1-210[»]
2UXUX-ray2.30A/B1-210[»]
2XDNX-ray2.20A/B/C/D1-210[»]
ProteinModelPortaliQ9AIU0.
SMRiQ9AIU0. Positions 5-210.

Miscellaneous databases

EvolutionaryTraceiQ9AIU0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7061HTH tetR-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

KOiK03577.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]
PfamiPF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AIU0-1 [UniParc]FASTAAdd to Basket

« Hide

MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW    50
HFNNKAELVQ ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL 100
VLDARTRRIN EILHHKCEFT DDMCEIRQQR QSAVLDCHKG ITLALANAVR 150
RGQLPGELDA ERAAVAMFAY VDGLIRRWLL LPDSVDLLGD VEKWVDTGLD 200
MLRLSPALRK 210
Length:210
Mass (Da):23,854
Last modified:June 1, 2001 - v1
Checksum:i237211F9046B9F9E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1.
CP003734 Genomic DNA. Translation: AFO46103.1.
RefSeqiWP_014859138.1. NC_018220.1.
YP_006530894.1. NC_018220.1.

Genome annotation databases

EnsemblBacteriaiAFO46103; AFO46103; T1E_0244.
GeneIDi13341873.
KEGGippx:T1E_0244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1 .
CP003734 Genomic DNA. Translation: AFO46103.1 .
RefSeqi WP_014859138.1. NC_018220.1.
YP_006530894.1. NC_018220.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2UXH X-ray 2.40 A/B 1-210 [» ]
2UXI X-ray 2.50 A/B 1-210 [» ]
2UXO X-ray 2.70 A/B 1-210 [» ]
2UXP X-ray 2.70 A/B 1-210 [» ]
2UXU X-ray 2.30 A/B 1-210 [» ]
2XDN X-ray 2.20 A/B/C/D 1-210 [» ]
ProteinModelPortali Q9AIU0.
SMRi Q9AIU0. Positions 5-210.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFO46103 ; AFO46103 ; T1E_0244 .
GeneIDi 13341873.
KEGGi ppx:T1E_0244.

Phylogenomic databases

KOi K03577.

Miscellaneous databases

EvolutionaryTracei Q9AIU0.

Family and domain databases

Gene3Di 1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProi IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view ]
Pfami PF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view ]
PRINTSi PR00455. HTHTETR.
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEi PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida."
    Duque E., Segura A., Mosqueda G., Ramos J.L.
    Mol. Microbiol. 39:1100-1106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: DOT-T1E.
  2. "Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
    Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
    Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DOT-T1E.
  3. "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR."
    Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.
    Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, INDUCTION BY ANTIBIOTICS.
    Strain: DOT-T1E.

Entry informationi

Entry nameiTTGR_PSEPT
AccessioniPrimary (citable) accession number: Q9AIU0
Secondary accession number(s): I7B485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi