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Q9AIU0 (TTGR_PSEPT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HTH-type transcriptional regulator TtgR
Alternative name(s):
Toluene efflux pump ttgABC operon repressor
Gene names
Name:ttgR
Ordered Locus Names:T1E_0244
OrganismPseudomonas putida (strain DOT-T1E) [Complete proteome] [HAMAP]
Taxonomic identifier1196325 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics. Ref.1

Subunit structure

Homodimer By similarity.

Induction

Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline. Ref.3

Sequence similarities

Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   LigandDNA-binding
   Molecular functionRepressor
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210HTH-type transcriptional regulator TtgR
PRO_0000070623

Regions

Domain10 – 7061HTH tetR-type
DNA binding33 – 5220H-T-H motif Potential

Secondary structure

............................ 210
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9AIU0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 237211F9046B9F9E

FASTA21023,854
        10         20         30         40         50         60 
MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW HFNNKAELVQ 

        70         80         90        100        110        120 
ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL VLDARTRRIN EILHHKCEFT 

       130        140        150        160        170        180 
DDMCEIRQQR QSAVLDCHKG ITLALANAVR RGQLPGELDA ERAAVAMFAY VDGLIRRWLL 

       190        200        210 
LPDSVDLLGD VEKWVDTGLD MLRLSPALRK 

« Hide

References

« Hide 'large scale' references
[1]"Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida."
Duque E., Segura A., Mosqueda G., Ramos J.L.
Mol. Microbiol. 39:1100-1106(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: DOT-T1E.
[2]"Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DOT-T1E.
[3]"Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR."
Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.
Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING, INDUCTION BY ANTIBIOTICS.
Strain: DOT-T1E.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF238479 Genomic DNA. Translation: AAK15050.1.
CP003734 Genomic DNA. Translation: AFO46103.1.
RefSeqYP_006530894.1. NC_018220.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2UXHX-ray2.40A/B1-210[»]
2UXIX-ray2.50A/B1-210[»]
2UXOX-ray2.70A/B1-210[»]
2UXPX-ray2.70A/B1-210[»]
2UXUX-ray2.30A/B1-210[»]
2XDNX-ray2.20A/B/C/D1-210[»]
ProteinModelPortalQ9AIU0.
SMRQ9AIU0. Positions 5-210.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFO46103; AFO46103; T1E_0244.
GeneID13341873.
KEGGppx:T1E_0244.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03577.

Family and domain databases

Gene3D1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]
PfamPF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSPR00455. HTHTETR.
SUPFAMSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9AIU0.

Entry information

Entry nameTTGR_PSEPT
AccessionPrimary (citable) accession number: Q9AIU0
Secondary accession number(s): I7B485
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references