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Q9AIU0

- TTGR_PSEPT

UniProt

Q9AIU0 - TTGR_PSEPT

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Protein

HTH-type transcriptional regulator TtgR

Gene

ttgR

Organism
Pseudomonas putida (strain DOT-T1E)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 5220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator TtgR
Alternative name(s):
Toluene efflux pump ttgABC operon repressor
Gene namesi
Name:ttgR
Ordered Locus Names:T1E_0244
OrganismiPseudomonas putida (strain DOT-T1E)
Taxonomic identifieri1196325 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006503: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210HTH-type transcriptional regulator TtgRPRO_0000070623Add
BLAST

Expressioni

Inductioni

Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2721Combined sources
Turni28 – 314Combined sources
Helixi34 – 418Combined sources
Helixi47 – 515Combined sources
Helixi55 – 6814Combined sources
Helixi71 – 777Combined sources
Helixi85 – 10218Combined sources
Helixi104 – 11512Combined sources
Turni122 – 1243Combined sources
Helixi125 – 15026Combined sources
Helixi160 – 18021Combined sources
Helixi182 – 1843Combined sources
Turni187 – 1904Combined sources
Helixi191 – 20414Combined sources
Helixi206 – 2083Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UXHX-ray2.40A/B1-210[»]
2UXIX-ray2.50A/B1-210[»]
2UXOX-ray2.70A/B1-210[»]
2UXPX-ray2.70A/B1-210[»]
2UXUX-ray2.30A/B1-210[»]
2XDNX-ray2.20A/B/C/D1-210[»]
ProteinModelPortaliQ9AIU0.
SMRiQ9AIU0. Positions 5-210.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AIU0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7061HTH tetR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK03577.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]
PfamiPF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AIU0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW
60 70 80 90 100
HFNNKAELVQ ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL
110 120 130 140 150
VLDARTRRIN EILHHKCEFT DDMCEIRQQR QSAVLDCHKG ITLALANAVR
160 170 180 190 200
RGQLPGELDA ERAAVAMFAY VDGLIRRWLL LPDSVDLLGD VEKWVDTGLD
210
MLRLSPALRK
Length:210
Mass (Da):23,854
Last modified:June 1, 2001 - v1
Checksum:i237211F9046B9F9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1.
CP003734 Genomic DNA. Translation: AFO46103.1.
RefSeqiWP_014859138.1. NC_018220.1.
YP_006530894.1. NC_018220.1.

Genome annotation databases

EnsemblBacteriaiAFO46103; AFO46103; T1E_0244.
GeneIDi13341873.
KEGGippx:T1E_0244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238479 Genomic DNA. Translation: AAK15050.1 .
CP003734 Genomic DNA. Translation: AFO46103.1 .
RefSeqi WP_014859138.1. NC_018220.1.
YP_006530894.1. NC_018220.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2UXH X-ray 2.40 A/B 1-210 [» ]
2UXI X-ray 2.50 A/B 1-210 [» ]
2UXO X-ray 2.70 A/B 1-210 [» ]
2UXP X-ray 2.70 A/B 1-210 [» ]
2UXU X-ray 2.30 A/B 1-210 [» ]
2XDN X-ray 2.20 A/B/C/D 1-210 [» ]
ProteinModelPortali Q9AIU0.
SMRi Q9AIU0. Positions 5-210.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFO46103 ; AFO46103 ; T1E_0244 .
GeneIDi 13341873.
KEGGi ppx:T1E_0244.

Phylogenomic databases

KOi K03577.

Miscellaneous databases

EvolutionaryTracei Q9AIU0.

Family and domain databases

Gene3Di 1.10.10.60. 2 hits.
1.10.357.10. 1 hit.
InterProi IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view ]
Pfami PF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view ]
PRINTSi PR00455. HTHTETR.
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEi PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida."
    Duque E., Segura A., Mosqueda G., Ramos J.L.
    Mol. Microbiol. 39:1100-1106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: DOT-T1E.
  2. "Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
    Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
    Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DOT-T1E.
  3. "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR."
    Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.
    Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, INDUCTION BY ANTIBIOTICS.
    Strain: DOT-T1E.

Entry informationi

Entry nameiTTGR_PSEPT
AccessioniPrimary (citable) accession number: Q9AIU0
Secondary accession number(s): I7B485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3