Q9AIU0 (TTGR_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator TtgR

Alternative name(s):
Toluene efflux pump ttgABC operon repressor

Gene names

Name:

ttgR

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics. Ref.1
Subunit structure	Homodimer By similarity.
Induction	Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline. Ref.2
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 210

210

HTH-type transcriptional regulator TtgR

PRO_0000070623

Regions

Domain

10 – 70

61

HTH tetR-type

DNA binding

33 – 52

20

H-T-H motif Potential

Secondary structure

1

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210

Helix Strand Turn

Details...

Helix

7 – 27

21

Turn

28 – 31

4

Helix

34 – 41

8

Helix

47 – 51

5

Helix

55 – 68

14

Helix

71 – 77

7

Helix

85 – 102

18

Helix

104 – 115

12

Turn

122 – 124

3

Helix

125 – 150

26

Helix

160 – 180

21

Helix

182 – 184

3

Turn

187 – 190

4

Helix

191 – 204

14

Helix

206 – 208

3

Sequences

	Sequence		Length	Mass (Da)
	Q9AIU0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 237211F9046B9F9E	FASTA	210	23,854
	10 20 30 40 50 60 MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW HFNNKAELVQ 70 80 90 100 110 120 ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL VLDARTRRIN EILHHKCEFT 130 140 150 160 170 180 DDMCEIRQQR QSAVLDCHKG ITLALANAVR RGQLPGELDA ERAAVAMFAY VDGLIRRWLL 190 200 210 LPDSVDLLGD VEKWVDTGLD MLRLSPALRK

References

[1]	"Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida." Duque E., Segura A., Mosqueda G., Ramos J.L. Mol. Microbiol. 39:1100-1106(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: DOT-T1E.
[2]	"Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR." Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T. Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING, INDUCTION BY ANTIBIOTICS. Strain: DOT-T1E.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF238479 Genomic DNA. Translation: AAK15050.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2UXH	X-ray	2.40	A/B	1-210	[»]
2UXI	X-ray	2.50	A/B	1-210	[»]
2UXO	X-ray	2.70	A/B	1-210	[»]
2UXP	X-ray	2.70	A/B	1-210	[»]
2UXU	X-ray	2.30	A/B	1-210	[»]
2XDN	X-ray	2.20	A/B/C/D	1-210	[»]

ProteinModelPortal

Q9AIU0.

SMR

Q9AIU0. Positions 5-210.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.10.60. 2 hits.
1.10.357.10. 1 hit.

InterPro

IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013572. Tscrpt_reg_MAATS_C.
[Graphical view]

Pfam

PF08361. TetR_C_2. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]

PRINTS

PR00455. HTHTETR.

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF48498. TetR_like_C. 1 hit.

PROSITE

PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9AIU0.

Entry information

Entry name

TTGR_PSEPU

Accession

Primary (citable) accession number: Q9AIU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program