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Q9AIU0

- TTGR_PSEPT

UniProt

Q9AIU0 - TTGR_PSEPT

Protein

HTH-type transcriptional regulator TtgR

Gene

ttgR

Organism
Pseudomonas putida (strain DOT-T1E)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Represses expression from the ttgABC operon promoter and its own expression. Binds to a promoter region between the divergently transcribed ttgR and ttgABC genes/operons; in the presence of chloramphenicol or tetracycline this binding no longer occurs and ttgR and ttgABC are derepressed. This suggests that TtgR binds these antibiotics.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi33 – 5220H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    HTH-type transcriptional regulator TtgR
    Alternative name(s):
    Toluene efflux pump ttgABC operon repressor
    Gene namesi
    Name:ttgR
    Ordered Locus Names:T1E_0244
    OrganismiPseudomonas putida (strain DOT-T1E)
    Taxonomic identifieri1196325 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000006503: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210HTH-type transcriptional regulator TtgRPRO_0000070623Add
    BLAST

    Expressioni

    Inductioni

    Induced 4- to 8-fold in the presence of toluene; this is controlled by TtgX, a possible efflux pump global regulator. Induced by chloramphenicol and probably by tetracycline.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    Secondary structure

    1
    210
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2721
    Turni28 – 314
    Helixi34 – 418
    Helixi47 – 515
    Helixi55 – 6814
    Helixi71 – 777
    Helixi85 – 10218
    Helixi104 – 11512
    Turni122 – 1243
    Helixi125 – 15026
    Helixi160 – 18021
    Helixi182 – 1843
    Turni187 – 1904
    Helixi191 – 20414
    Helixi206 – 2083

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2UXHX-ray2.40A/B1-210[»]
    2UXIX-ray2.50A/B1-210[»]
    2UXOX-ray2.70A/B1-210[»]
    2UXPX-ray2.70A/B1-210[»]
    2UXUX-ray2.30A/B1-210[»]
    2XDNX-ray2.20A/B/C/D1-210[»]
    ProteinModelPortaliQ9AIU0.
    SMRiQ9AIU0. Positions 5-210.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9AIU0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 7061HTH tetR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK03577.

    Family and domain databases

    Gene3Di1.10.10.60. 2 hits.
    1.10.357.10. 1 hit.
    InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
    IPR009057. Homeodomain-like.
    IPR001647. HTH_TetR.
    IPR015893. Tet_transcr_reg_TetR-like_C.
    IPR011075. Tet_transcr_reg_TetR-rel_C.
    IPR013572. Tscrpt_reg_MAATS_C.
    [Graphical view]
    PfamiPF08361. TetR_C_2. 1 hit.
    PF00440. TetR_N. 1 hit.
    [Graphical view]
    PRINTSiPR00455. HTHTETR.
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF48498. SSF48498. 1 hit.
    PROSITEiPS01081. HTH_TETR_1. 1 hit.
    PS50977. HTH_TETR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AIU0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRRTKEEAQ ETRAQIIEAA ERAFYKRGVA RTTLADIAEL AGVTRGAIYW    50
    HFNNKAELVQ ALLDSLHETH DHLARASESE DEVDPLGCMR KLLLQVFNEL 100
    VLDARTRRIN EILHHKCEFT DDMCEIRQQR QSAVLDCHKG ITLALANAVR 150
    RGQLPGELDA ERAAVAMFAY VDGLIRRWLL LPDSVDLLGD VEKWVDTGLD 200
    MLRLSPALRK 210
    Length:210
    Mass (Da):23,854
    Last modified:June 1, 2001 - v1
    Checksum:i237211F9046B9F9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF238479 Genomic DNA. Translation: AAK15050.1.
    CP003734 Genomic DNA. Translation: AFO46103.1.
    RefSeqiWP_014859138.1. NC_018220.1.
    YP_006530894.1. NC_018220.1.

    Genome annotation databases

    EnsemblBacteriaiAFO46103; AFO46103; T1E_0244.
    GeneIDi13341873.
    KEGGippx:T1E_0244.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF238479 Genomic DNA. Translation: AAK15050.1 .
    CP003734 Genomic DNA. Translation: AFO46103.1 .
    RefSeqi WP_014859138.1. NC_018220.1.
    YP_006530894.1. NC_018220.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2UXH X-ray 2.40 A/B 1-210 [» ]
    2UXI X-ray 2.50 A/B 1-210 [» ]
    2UXO X-ray 2.70 A/B 1-210 [» ]
    2UXP X-ray 2.70 A/B 1-210 [» ]
    2UXU X-ray 2.30 A/B 1-210 [» ]
    2XDN X-ray 2.20 A/B/C/D 1-210 [» ]
    ProteinModelPortali Q9AIU0.
    SMRi Q9AIU0. Positions 5-210.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFO46103 ; AFO46103 ; T1E_0244 .
    GeneIDi 13341873.
    KEGGi ppx:T1E_0244.

    Phylogenomic databases

    KOi K03577.

    Miscellaneous databases

    EvolutionaryTracei Q9AIU0.

    Family and domain databases

    Gene3Di 1.10.10.60. 2 hits.
    1.10.357.10. 1 hit.
    InterProi IPR023772. DNA-bd_HTH_TetR-type_CS.
    IPR009057. Homeodomain-like.
    IPR001647. HTH_TetR.
    IPR015893. Tet_transcr_reg_TetR-like_C.
    IPR011075. Tet_transcr_reg_TetR-rel_C.
    IPR013572. Tscrpt_reg_MAATS_C.
    [Graphical view ]
    Pfami PF08361. TetR_C_2. 1 hit.
    PF00440. TetR_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00455. HTHTETR.
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF48498. SSF48498. 1 hit.
    PROSITEi PS01081. HTH_TETR_1. 1 hit.
    PS50977. HTH_TETR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida."
      Duque E., Segura A., Mosqueda G., Ramos J.L.
      Mol. Microbiol. 39:1100-1106(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: DOT-T1E.
    2. "Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
      Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
      Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DOT-T1E.
    3. "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR."
      Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.
      Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING, INDUCTION BY ANTIBIOTICS.
      Strain: DOT-T1E.

    Entry informationi

    Entry nameiTTGR_PSEPT
    AccessioniPrimary (citable) accession number: Q9AIU0
    Secondary accession number(s): I7B485
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3