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Protein

Putative toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM2

Gene

tsaM2

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: -Protein uncertaini

Functioni

Involved in the toluene-4-sulfonate degradation pathway. Does not discriminate between the sulfonate and the carboxyl substituents and can also be involved in the p-toluenecarboxylate degradation pathway (By similarity).By similarity1 Publication

Caution

Could be the product of a pseudogene. Probably not expressed, due to the absence of promoter-like sequences upstream of the operon tsaMBCD2 (PubMed:11282598).1 Publication

Catalytic activityi

Toluene-4-sulfonate + FADH2 + O2 = 4-sulfobenzyl alcohol + H2O + FAD.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi49Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi66Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi69Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM2 (EC:1.14.14.-)
Alternative name(s):
Toluenesulfonate methyl-monooxygenase oxygenase component TsaM2
Gene namesi
Name:tsaM2
Encoded oniPlasmid pTSA0 Publication
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004191201 – 346Putative toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM2Add BLAST346

Proteomic databases

PRIDEiQ9AHG3

Interactioni

Subunit structurei

Homotetramer. Part of the p-toluenesulfonate methyl-monooxygenase complex TsaBM, comprising the reductase TsaB and the oxygenase TsaM.

Structurei

3D structure databases

ProteinModelPortaliQ9AHG3
SMRiQ9AHG3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 108RieskePROSITE-ProRule annotationAdd BLAST102

Family and domain databases

Gene3Di2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR015879 Ring_hydroxy_dOase_asu_C_dom
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF00848 Ring_hydroxyl_A, 1 hit
SUPFAMiSSF50022 SSF50022, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9AHG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVKNTWYVA GMATDCSRKP LARTFLNEKV VLFRTHDGHA VALEDRCCHR
60 70 80 90 100
LAPLSLGDVE DAGIRCRYHG MVFNASGACV EIPGQEQIPP GMCVRRFPLV
110 120 130 140 150
ERHGLLWIWM GDPARANPDD IVDELWNGAP EWRTDSGYIH YQANYQLIVD
160 170 180 190 200
NLLDFTHLAW VHPTTLGTDS AASLKPVIER DTTGTGKLTI TRWYLNDDMS
210 220 230 240 250
NLHKGVAKFE GKADRWQIYQ WSPPALLRMD TGSAPTGTGA PEGRRVPEAV
260 270 280 290 300
QFRHTSIQTP ETETTSHYWF CQARNFDLDD EALTEKIYQG VVVAFEEDRT
310 320 330 340
MIEAQQKILS QVPDRPMVPI AADAGLNQGR WLLDRLLKAE NGGTAP
Length:346
Mass (Da):38,884
Last modified:June 1, 2001 - v1
Checksum:i0222DA7E58859CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA Translation: AAK37996.1

Similar proteinsi

Entry informationi

Entry nameiTSAM2_COMTE
AccessioniPrimary (citable) accession number: Q9AHG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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