Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9AGJ6

- PCKG_MYCSM

UniProt

Q9AGJ6 - PCKG_MYCSM

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium smegmatis
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.

    Enzyme regulationi

    Inhibited by oxalate and by alpha-ketoglutarate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei222 – 2221Substrate; via amide nitrogenBy similarity
    Metal bindingi229 – 2291ManganeseBy similarity
    Binding sitei229 – 2291SubstrateBy similarity
    Metal bindingi249 – 2491Manganese; via tele nitrogenBy similarity
    Binding sitei271 – 2711SubstrateBy similarity
    Active sitei273 – 2731By similarity
    Metal bindingi296 – 2961ManganeseBy similarity
    Binding sitei388 – 3881GTPBy similarity
    Binding sitei419 – 4191GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi272 – 2776GTPBy similarity
    Nucleotide bindingi514 – 5174GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi4.1.1.32. 3512.
    SABIO-RKQ9AGJ6.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
    Short name:
    PEP carboxykinase
    Short name:
    PEPCK
    Gene namesi
    Name:pckG
    Synonyms:pck
    OrganismiMycobacterium smegmatis
    Taxonomic identifieri1772 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103610Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AGJ6.
    SMRiQ9AGJ6. Positions 12-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni386 – 3883Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AGJ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSATIPGLD TAPTKHQGLL AWVQEVAELT QPDRVVFADG SDEEYERLCA    50
    HLVEAGTFQK LNPEKQPNSY LALSDPSDVA RVESRTFICT EREIDAGPTN 100
    NWMDPAEMRG IMTDLYRGSM RGRTLYVVPF CMGPLDAEDP KLGVEITDSE 150
    YVVVSMRTMT RMGRAALDKL GDDGFFVKAL HSIGAPLEPG QKDVPWPCND 200
    TKYITHFPET REIWSFGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM 250
    LILKLISPEN KAYFIAAAFP SACGKTNLAM LQPTIEGWRA ETVGDDIAWM 300
    RFGKDGRLYA TNPEFGFFGV APGTNWSSNP NAMKTIAAGN TVFTNVAKTD 350
    DGDVWWEGLE GDPQHLIDWK GNDWTPESGE KAAHPNSRYC TPISQCPTLA 400
    PEWDDPQGVP ISAILFGGRR KTTVPLITEA RDWQHGVFIG ATLGSEQTAA 450
    AEGKVGTVRR DPMAMLPFLG YNVGDYFAHW INVGKNADES KLPKVFFVNW 500
    FRRGDDGRFL WPGFGENSRV LKWAVERIEH KADGKSTPIG IVPTAADLDL 550
    EGLDVDPADV DEALAVKPEE WRAELPLIEE WFEFVGEKLP TGLKDEFDAL 600
    KERLG 605
    Length:605
    Mass (Da):66,944
    Last modified:June 1, 2001 - v1
    Checksum:iE07A46D4FE35EDC0
    GO

    Mass spectrometryi

    Molecular mass is 71209 Da from positions 1 - 605. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF332191 Genomic DNA. Translation: AAK28534.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF332191 Genomic DNA. Translation: AAK28534.1 .

    3D structure databases

    ProteinModelPortali Q9AGJ6.
    SMRi Q9AGJ6. Positions 12-604.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BRENDAi 4.1.1.32. 3512.
    SABIO-RK Q9AGJ6.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A GTP-dependent vertebrate-type phosphoenolpyruvate carboxykinase from Mycobacterium smegmatis."
      Mukhopadhyay B., Concar E.M., Wolfe R.S.
      J. Biol. Chem. 276:16137-16145(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPCKG_MYCSM
    AccessioniPrimary (citable) accession number: Q9AGJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3