D-aminoacylase - Alcaligenes faecalis

Preferred order of sections

Names and origin

Protein names	Submitted name: D-aminoacylase EMBL AAK15530.1 EC=3.5.1.81 EMBL AAK15530.1
Organism	Alcaligenes faecalis EMBL AAK15530.1
Taxonomic identifier	511 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Copper PDB 1RJP PDB 1RK5 Metal-binding PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V4Y PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ Zinc PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V4Y PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ
Molecular function	Hydrolase EMBL AAK15530.1
Technical term	3D-structure PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V4Y PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ
Gene Ontology (GO)
Molecular_function	N-acyl-D-amino-acid deacylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

68

1

Copper; via tele nitrogen

Metal binding

68

1

Zinc 1; via tele nitrogen PDB 1RJR PDB 1M7J PDB 1V4Y PDB 1V51

Metal binding

70

1

Copper; via tele nitrogen

Metal binding

70

1

Zinc 1; via tele nitrogen PDB 1RJR PDB 1M7J PDB 1V4Y PDB 1V51

Metal binding

78

1

Zinc 2; via tele nitrogen PDB 1V51

Metal binding

97

1

Copper

Metal binding

97

1

Zinc 1 PDB 1RJR PDB 1M7J PDB 1V4Y

Metal binding

97

1

Zinc 3 PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ

Metal binding

118

1

Zinc 2 PDB 1V51

Metal binding

177

1

Zinc 4 PDB 1V51

Metal binding

202

1

Zinc 5 PDB 1V51

Metal binding

206

1

Zinc 5 PDB 1V51

Metal binding

214

1

Zinc 4; via tele nitrogen PDB 1V51

Metal binding

221

1

Zinc 3; via pros nitrogen PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ

Metal binding

251

1

Zinc 3; via tele nitrogen PDB 1RJR PDB 1M7J PDB 1RK6 PDB 1V51 PDB 1RJP PDB 1RK5 PDB 1RJQ

Metal binding

322

1

Zinc 6 PDB 1V51

Metal binding

326

1

Zinc 6 PDB 1V51

Metal binding

367

1

Zinc 1 PDB 1V4Y PDB 1V51

Sequences

Sequence

Length

Mass (Da)

Tools

Q9AGH8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 634119A6CB75155E
Show »

FASTA

484

52,001

        10         20         30         40         50         60 
MSQPDATPFD YILSGGTVID GTNAPGRLAD VGVRGDRIAA VGDLSASSAR RRIDVAGKVV 

        70         80         90        100        110        120 
SPGFIDSHTH DDNYLLKHRD MTPKISQGVT TVVTGNCGIS LAPLAHANPP APLDLLDEGG 

       130        140        150        160        170        180 
SFRFARFSDY LEALRAAPPA VNAACMVGHS TLRAAVMPDL RREATADEIQ AMQALADDAL 

       190        200        210        220        230        240 
ASGAIGISTG AFYPPAAHAS TEEIIEVCRP LITHGGVYAT HMRDEGEHIV QALEETFRIG 

       250        260        270        280        290        300 
RELDVPVVIS HHKVMGKLNF GRSKETLALI EAAMASQDVS LDAYPYVAGS TMLKQDRVLL 

       310        320        330        340        350        360 
AGRTLITWCK PYPELSGRDL EEIAAERGKS KYDVVPELQP AGAIYFMMDE PDVQRILAFG 

       370        380        390        400        410        420 
PTMIGSDGLP HDERPHPRLW GTFPRVLGHY SRDLGLFPLE TAVWKMTGLT AAKFGLAERG 

       430        440        450        460        470        480 
QVQPGYYADL VVFDPATVAD SATFEHPTER AAGIHSVYVN GAAVWEDQSF TGQHAGRVLN 


RAGA

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References

[1]	"Structural-based mutational analysis of D-aminoacylase from Alcaligenes faecalis DA1." Hsu C.S., Lai W.L., Chang W.W., Liaw S.H., Tsai Y.C. Protein Sci. 11:2545-2550(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1. A novel subset of amidohydrolases and insights into the enzyme mechanism." Liaw S.H., Chen S.J., Ko T.P., Hsu C.S., Chen C.J., Wang A.H., Tsai Y.C. J. Biol. Chem. 278:4957-4962(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH ZINC.
[3]	"The functional role of the binuclear metal center in D-aminoacylase: one-metal activation and second-metal attenuation." Lai W.L., Chou L.Y., Ting C.Y., Kirby R., Tsai Y.C., Wang A.H., Liaw S.H. J. Biol. Chem. 279:13962-13967(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.43 ANGSTROMS) IN COMPLEX WITH COPPER AND ZINC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF332548 Genomic DNA. Translation: AAK15530.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M7J	X-ray	1.50	A	1-484	[»]
1RJP	X-ray	1.80	A	1-484	[»]
1RJQ	X-ray	1.80	A	1-484	[»]
1RJR	X-ray	2.10	A	1-484	[»]
1RK5	X-ray	1.80	A	1-484	[»]
1RK6	X-ray	1.43	A	1-484	[»]
1V4Y	X-ray	1.65	A	1-484	[»]
1V51	X-ray	1.60	A	1-484	[»]

ProteinModelPortal

Q9AGH8.

SMR

Q9AGH8. Positions 8-481.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.1490.130. 1 hit.

InterPro

IPR013108. Amidohydro_3.
IPR023100. D-aminoacylase_insert_dom.
IPR012855. D_aminoacylase_C.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]

Pfam

PF07969. Amidohydro_3. 2 hits.
PF07908. D-aminoacyl_C. 1 hit.
[Graphical view]

SUPFAM

SSF51338. Metalo_hydrolase. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9AGH8.

Entry information

Entry name

Q9AGH8_ALCFA

Accession

Primary (citable) accession number: Q9AGH8

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)