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Protein

6-phospho-alpha-glucosidase

Gene

aglB

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'-phosphate, isomaltose-6'-phosphate, maltitol-6-phosphate, trehalose-6-phosphate and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate. However, sucrose-6-phosphate is not a substrate for this enzyme.2 Publications

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD(+)1 Publication
  • Mn2+1 Publication, Co2+1 Publication, Ni2+1 PublicationNote: Divalent metal cation. Requires Mn(2+), Co(2+) or Ni2+.1 Publication

Kineticsi

  1. KM=1.23 mM for trehalulose-6'-phosphate1 Publication
  2. KM=1.68 mM for turanose-6'-phosphate1 Publication
  3. KM=1.20 mM for maltulose-6'-phosphate1 Publication
  4. KM=5.63 mM for leucrose-6'-phosphate1 Publication
  5. KM=2.42 mM for palatinose-6'-phosphate1 Publication
  6. KM=3.08 mM for maltose-6'-phosphate1 Publication
  7. KM=4.48 mM for isomaltose-6'-phosphate1 Publication
  8. KM=0.82 mM for maltitol-6-phosphate1 Publication
  9. KM=1.16 mM for trehalose-6-phosphate1 Publication
  10. KM=0.05 mM for p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate1 Publication
  1. Vmax=0.89 µmol/min/mg enzyme with trehalulose-6'-phosphate as substrate1 Publication
  2. Vmax=2.41 µmol/min/mg enzyme with turanose-6'-phosphate as substrate1 Publication
  3. Vmax=1.15 µmol/min/mg enzyme with maltulose-6'-phosphate as substrate1 Publication
  4. Vmax=0.85 µmol/min/mg enzyme with leucrose-6'-phosphate as substrate1 Publication
  5. Vmax=0.90 µmol/min/mg enzyme with palatinose-6'-phosphate as substrate1 Publication
  6. Vmax=1.31 µmol/min/mg enzyme with maltose-6'-phosphate as substrate1 Publication
  7. Vmax=1.55 µmol/min/mg enzyme with isomaltose-6'-phosphate as substrate1 Publication
  8. Vmax=1.87 µmol/min/mg enzyme with maltitol-6-phosphate as substrate1 Publication
  9. Vmax=0.31 µmol/min/mg enzyme with trehalose-6-phosphate as substrate1 Publication
  10. Vmax=2.42 µmol/min/mg enzyme with p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate as substrate1 Publication

Pathway:isucrose metabolism

This protein is involved in the pathway sucrose metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sucrose metabolism and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateBy similarity
Sitei109 – 1091Increases basicity of active site TyrBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Metal bindingi169 – 1691ManganeseBy similarity
Active sitei170 – 1701Proton donorBy similarity
Metal bindingi200 – 2001ManganeseBy similarity
Active sitei263 – 2631Proton acceptorBy similarity
Binding sitei283 – 2831SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 7067NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BRENDAi3.2.1.122. 2814.
SABIO-RKQ9AGA6.
UniPathwayiUPA00238.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-alpha-glucosidase (EC:3.2.1.122)
Gene namesi
Name:aglB
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4404406-phospho-alpha-glucosidasePRO_0000169856Add
BLAST

Expressioni

Inductioni

By the five linkage-isomeric alpha-D-glucosyl-D-fructoses, or by maltose or maltitol.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi272620.KPN_04085.

Structurei

3D structure databases

ProteinModelPortaliQ9AGA6.
SMRiQ9AGA6. Positions 2-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AGA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFSVVIAG GGSTFTPGIV LMLLANQDRF PLRSLKFYDN DGARQETIAE
60 70 80 90 100
ACKVILKEQA PEIEFSYTTD PQAAFTDVDF VMAHIRVGKY PMREQDEKIP
110 120 130 140 150
LRHGVLGQET CGPGGIAYGM RSIGGVLELV DYMEKYSPNA WMLNYSNPAA
160 170 180 190 200
IVAEATRRLR PNAKILNICD MPIGIEGRMA QIVGLKDRKQ MRVRYYGLNH
210 220 230 240 250
FGWWTSIEDL DGNDLMPKLR EYVAKYGYVP PSNDPHTEAS WNDTFAKAKD
260 270 280 290 300
VQALDPQTMP NTYLKYYLFP DYVVAHSNPE RTRANEVMDH REKNVFSACR
310 320 330 340 350
AIIAAGKSTA GDLEIDEHAS YIVDLATAIA FNTQERMLLI VPNNGAIHNF
360 370 380 390 400
DADAMVEIPC LVGHNGPEPL TVGDIPHFQK GLMSQQVAVE KLVVDAWEQR
410 420 430 440
SYHKLWQAIT LSKTVPSASV AKAILDDLIA ANKDYWPELH
Length:440
Mass (Da):49,255
Last modified:June 1, 2001 - v1
Checksum:i5D7287435E4DDC45
GO

Mass spectrometryi

Molecular mass is 49254 Da from positions 1 - 440. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF337811 Genomic DNA. Translation: AAK01457.1.
RefSeqiWP_002923306.1. NZ_LART01000007.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF337811 Genomic DNA. Translation: AAK01457.1.
RefSeqiWP_002923306.1. NZ_LART01000007.1.

3D structure databases

ProteinModelPortaliQ9AGA6.
SMRiQ9AGA6. Positions 2-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_04085.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00238.
BRENDAi3.2.1.122. 2814.
SABIO-RKQ9AGA6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase."
    Thompson J., Robrish S.A., Immel S., Lichtenthaler F.W., Hall B.G., Pikis A.
    J. Biol. Chem. 276:37415-37425(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26, FUNCTION, CHARACTERIZATION, COFACTOR, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS, INDUCTION, SUBUNIT, MASS SPECTROMETRY.
    Strain: ATCC 23357 / A-11.
  2. "Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae."
    Thompson J., Robrish S.A., Pikis A., Brust A., Lichtenthaler F.W.
    Carbohydr. Res. 331:149-161(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-25, FUNCTION, INDUCTION.
    Strain: ATCC 23357 / A-11.

Entry informationi

Entry nameiAGLB_KLEPN
AccessioniPrimary (citable) accession number: Q9AGA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.