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Q9AGA6

- AGLB_KLEPN

UniProt

Q9AGA6 - AGLB_KLEPN

Protein

6-phospho-alpha-glucosidase

Gene

aglB

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'-phosphate, isomaltose-6'-phosphate, maltitol-6-phosphate, trehalose-6-phosphate and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate. However, sucrose-6-phosphate is not a substrate for this enzyme.2 Publications

    Catalytic activityi

    Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

    Cofactori

    NAD.1 Publication
    Divalent metal ion. Requires Mn2+, Co2+ or Ni2+.1 Publication

    Kineticsi

    1. KM=1.23 mM for trehalulose-6'-phosphate1 Publication
    2. KM=1.68 mM for turanose-6'-phosphate1 Publication
    3. KM=1.20 mM for maltulose-6'-phosphate1 Publication
    4. KM=5.63 mM for leucrose-6'-phosphate1 Publication
    5. KM=2.42 mM for palatinose-6'-phosphate1 Publication
    6. KM=3.08 mM for maltose-6'-phosphate1 Publication
    7. KM=4.48 mM for isomaltose-6'-phosphate1 Publication
    8. KM=0.82 mM for maltitol-6-phosphate1 Publication
    9. KM=1.16 mM for trehalose-6-phosphate1 Publication
    10. KM=0.05 mM for p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate1 Publication

    Vmax=0.89 µmol/min/mg enzyme with trehalulose-6'-phosphate as substrate1 Publication

    Vmax=2.41 µmol/min/mg enzyme with turanose-6'-phosphate as substrate1 Publication

    Vmax=1.15 µmol/min/mg enzyme with maltulose-6'-phosphate as substrate1 Publication

    Vmax=0.85 µmol/min/mg enzyme with leucrose-6'-phosphate as substrate1 Publication

    Vmax=0.90 µmol/min/mg enzyme with palatinose-6'-phosphate as substrate1 Publication

    Vmax=1.31 µmol/min/mg enzyme with maltose-6'-phosphate as substrate1 Publication

    Vmax=1.55 µmol/min/mg enzyme with isomaltose-6'-phosphate as substrate1 Publication

    Vmax=1.87 µmol/min/mg enzyme with maltitol-6-phosphate as substrate1 Publication

    Vmax=0.31 µmol/min/mg enzyme with trehalose-6-phosphate as substrate1 Publication

    Vmax=2.42 µmol/min/mg enzyme with p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate as substrate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateBy similarity
    Sitei109 – 1091Increases basicity of active site TyrBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Metal bindingi169 – 1691ManganeseBy similarity
    Active sitei170 – 1701Proton donorBy similarity
    Metal bindingi200 – 2001ManganeseBy similarity
    Active sitei263 – 2631Proton acceptorBy similarity
    Binding sitei283 – 2831SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi4 – 7067NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. maltose-6'-phosphate glucosidase activity Source: CACAO
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. sucrose metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Cobalt, Manganese, Metal-binding, NAD, Nickel

    Enzyme and pathway databases

    BRENDAi3.2.1.122. 2814.
    SABIO-RKQ9AGA6.
    UniPathwayiUPA00238.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-alpha-glucosidase (EC:3.2.1.122)
    Gene namesi
    Name:aglB
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4404406-phospho-alpha-glucosidasePRO_0000169856Add
    BLAST

    Expressioni

    Inductioni

    By the five linkage-isomeric alpha-D-glucosyl-D-fructoses, or by maltose or maltitol.2 Publications

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AGA6.
    SMRiQ9AGA6. Positions 2-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AGA6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKFSVVIAG GGSTFTPGIV LMLLANQDRF PLRSLKFYDN DGARQETIAE    50
    ACKVILKEQA PEIEFSYTTD PQAAFTDVDF VMAHIRVGKY PMREQDEKIP 100
    LRHGVLGQET CGPGGIAYGM RSIGGVLELV DYMEKYSPNA WMLNYSNPAA 150
    IVAEATRRLR PNAKILNICD MPIGIEGRMA QIVGLKDRKQ MRVRYYGLNH 200
    FGWWTSIEDL DGNDLMPKLR EYVAKYGYVP PSNDPHTEAS WNDTFAKAKD 250
    VQALDPQTMP NTYLKYYLFP DYVVAHSNPE RTRANEVMDH REKNVFSACR 300
    AIIAAGKSTA GDLEIDEHAS YIVDLATAIA FNTQERMLLI VPNNGAIHNF 350
    DADAMVEIPC LVGHNGPEPL TVGDIPHFQK GLMSQQVAVE KLVVDAWEQR 400
    SYHKLWQAIT LSKTVPSASV AKAILDDLIA ANKDYWPELH 440
    Length:440
    Mass (Da):49,255
    Last modified:June 1, 2001 - v1
    Checksum:i5D7287435E4DDC45
    GO

    Mass spectrometryi

    Molecular mass is 49254 Da from positions 1 - 440. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF337811 Genomic DNA. Translation: AAK01457.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF337811 Genomic DNA. Translation: AAK01457.1 .

    3D structure databases

    ProteinModelPortali Q9AGA6.
    SMRi Q9AGA6. Positions 2-439.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH4. Glycoside Hydrolase Family 4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00238 .
    BRENDAi 3.2.1.122. 2814.
    SABIO-RK Q9AGA6.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view ]
    PRINTSi PR00732. GLHYDRLASE4.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase."
      Thompson J., Robrish S.A., Immel S., Lichtenthaler F.W., Hall B.G., Pikis A.
      J. Biol. Chem. 276:37415-37425(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26, FUNCTION, CHARACTERIZATION, COFACTOR, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS, INDUCTION, SUBUNIT, MASS SPECTROMETRY.
      Strain: ATCC 23357 / A-11.
    2. "Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae."
      Thompson J., Robrish S.A., Pikis A., Brust A., Lichtenthaler F.W.
      Carbohydr. Res. 331:149-161(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-25, FUNCTION, INDUCTION.
      Strain: ATCC 23357 / A-11.

    Entry informationi

    Entry nameiAGLB_KLEPN
    AccessioniPrimary (citable) accession number: Q9AGA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3