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Q9AGA6

- AGLB_KLEPN

UniProt

Q9AGA6 - AGLB_KLEPN

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Protein
6-phospho-alpha-glucosidase
Gene
aglB
Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'-phosphate, isomaltose-6'-phosphate, maltitol-6-phosphate, trehalose-6-phosphate and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate. However, sucrose-6-phosphate is not a substrate for this enzyme.2 Publications

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

NAD.1 Publication
Divalent metal ion. Requires Mn2+, Co2+ or Ni2+.1 Publication

Kineticsi

  1. KM=1.23 mM for trehalulose-6'-phosphate1 Publication
  2. KM=1.68 mM for turanose-6'-phosphate
  3. KM=1.20 mM for maltulose-6'-phosphate
  4. KM=5.63 mM for leucrose-6'-phosphate
  5. KM=2.42 mM for palatinose-6'-phosphate
  6. KM=3.08 mM for maltose-6'-phosphate
  7. KM=4.48 mM for isomaltose-6'-phosphate
  8. KM=0.82 mM for maltitol-6-phosphate
  9. KM=1.16 mM for trehalose-6-phosphate
  10. KM=0.05 mM for p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate

Vmax=0.89 µmol/min/mg enzyme with trehalulose-6'-phosphate as substrate

Vmax=2.41 µmol/min/mg enzyme with turanose-6'-phosphate as substrate

Vmax=1.15 µmol/min/mg enzyme with maltulose-6'-phosphate as substrate

Vmax=0.85 µmol/min/mg enzyme with leucrose-6'-phosphate as substrate

Vmax=0.90 µmol/min/mg enzyme with palatinose-6'-phosphate as substrate

Vmax=1.31 µmol/min/mg enzyme with maltose-6'-phosphate as substrate

Vmax=1.55 µmol/min/mg enzyme with isomaltose-6'-phosphate as substrate

Vmax=1.87 µmol/min/mg enzyme with maltitol-6-phosphate as substrate

Vmax=0.31 µmol/min/mg enzyme with trehalose-6-phosphate as substrate

Vmax=2.42 µmol/min/mg enzyme with p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate as substrate

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931Substrate By similarity
Sitei109 – 1091Increases basicity of active site Tyr By similarity
Binding sitei147 – 1471Substrate By similarity
Metal bindingi169 – 1691Manganese By similarity
Active sitei170 – 1701Proton donor By similarity
Metal bindingi200 – 2001Manganese By similarity
Active sitei263 – 2631Proton acceptor By similarity
Binding sitei283 – 2831Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 7067NAD By similarity
Add
BLAST

GO - Molecular functioni

  1. maltose-6'-phosphate glucosidase activity Source: CACAO
  2. metal ion binding Source: UniProtKB-KW
  3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

GO - Biological processi

  1. sucrose metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BRENDAi3.2.1.122. 2814.
SABIO-RKQ9AGA6.
UniPathwayiUPA00238.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-alpha-glucosidase (EC:3.2.1.122)
Gene namesi
Name:aglB
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4404406-phospho-alpha-glucosidase
PRO_0000169856Add
BLAST

Expressioni

Inductioni

By the five linkage-isomeric alpha-D-glucosyl-D-fructoses, or by maltose or maltitol.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9AGA6.
SMRiQ9AGA6. Positions 2-439.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AGA6-1 [UniParc]FASTAAdd to Basket

« Hide

MKKFSVVIAG GGSTFTPGIV LMLLANQDRF PLRSLKFYDN DGARQETIAE    50
ACKVILKEQA PEIEFSYTTD PQAAFTDVDF VMAHIRVGKY PMREQDEKIP 100
LRHGVLGQET CGPGGIAYGM RSIGGVLELV DYMEKYSPNA WMLNYSNPAA 150
IVAEATRRLR PNAKILNICD MPIGIEGRMA QIVGLKDRKQ MRVRYYGLNH 200
FGWWTSIEDL DGNDLMPKLR EYVAKYGYVP PSNDPHTEAS WNDTFAKAKD 250
VQALDPQTMP NTYLKYYLFP DYVVAHSNPE RTRANEVMDH REKNVFSACR 300
AIIAAGKSTA GDLEIDEHAS YIVDLATAIA FNTQERMLLI VPNNGAIHNF 350
DADAMVEIPC LVGHNGPEPL TVGDIPHFQK GLMSQQVAVE KLVVDAWEQR 400
SYHKLWQAIT LSKTVPSASV AKAILDDLIA ANKDYWPELH 440
Length:440
Mass (Da):49,255
Last modified:June 1, 2001 - v1
Checksum:i5D7287435E4DDC45
GO

Mass spectrometryi

Molecular mass is 49254 Da from positions 1 - 440. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF337811 Genomic DNA. Translation: AAK01457.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF337811 Genomic DNA. Translation: AAK01457.1 .

3D structure databases

ProteinModelPortali Q9AGA6.
SMRi Q9AGA6. Positions 2-439.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00238 .
BRENDAi 3.2.1.122. 2814.
SABIO-RK Q9AGA6.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view ]
PRINTSi PR00732. GLHYDRLASE4.
SUPFAMi SSF56327. SSF56327. 1 hit.
PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase."
    Thompson J., Robrish S.A., Immel S., Lichtenthaler F.W., Hall B.G., Pikis A.
    J. Biol. Chem. 276:37415-37425(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26, FUNCTION, CHARACTERIZATION, COFACTOR, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS, INDUCTION, SUBUNIT, MASS SPECTROMETRY.
    Strain: ATCC 23357 / A-11.
  2. "Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae."
    Thompson J., Robrish S.A., Pikis A., Brust A., Lichtenthaler F.W.
    Carbohydr. Res. 331:149-161(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-25, FUNCTION, INDUCTION.
    Strain: ATCC 23357 / A-11.

Entry informationi

Entry nameiAGLB_KLEPN
AccessioniPrimary (citable) accession number: Q9AGA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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