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Protein

Xylulose-5-phosphate/fructose-6-phosphate phosphoketolase

Gene

xfp

Organism
Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phosphoketolase using both fructose 6-phosphate and xylulose 5-phosphate as substrate.

Catalytic activityi

D-xylulose 5-phosphate + phosphate = acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O.
D-fructose 6-phosphate + phosphate = acetyl phosphate + D-erythrose 4-phosphate + H2O.

Cofactori

Kineticsi

  1. KM=10 mM for fructose 6-phosphate
  2. KM=45 mM for xylulose 5-phosphate

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciBANI555970:GJ22-970-MONOMER.
    MetaCyc:MONOMER-12656.
    BRENDAi4.1.2.22. 6813.
    4.1.2.9. 6813.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (EC:4.1.2.22, EC:4.1.2.9)
    Gene namesi
    Name:xfp
    Ordered Locus Names:Balat_0971
    OrganismiBifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314)
    Taxonomic identifieri555970 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 825825Xylulose-5-phosphate/fructose-6-phosphate phosphoketolasePRO_0000193867Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AEM9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the XFP family.Curated

    Phylogenomic databases

    eggNOGiCOG3957.
    HOGENOMiHOG000236450.
    KOiK01621.
    OMAiVVKPRWP.
    OrthoDBiEOG61S2WD.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    HAMAPiMF_01403. Phosphoketolase.
    InterProiIPR023962. Phosphoketolase.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005593. Xul5P/Fru6P_PKetolase.
    IPR018969. Xul5P/Fru6P_PKetolase_C.
    IPR019790. Xul5P/Fru6P_PKetolase_CS.
    IPR018970. Xul5P/Fru6P_PKetolase_N.
    IPR019789. Xul5P/Fru6P_PKetolase_ThDP_BS.
    [Graphical view]
    PfamiPF03894. XFP. 1 hit.
    PF09363. XFP_C. 1 hit.
    PF09364. XFP_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017245. Phosphoketolase. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    PROSITEiPS60002. PHOSPHOKETOLASE_1. 1 hit.
    PS60003. PHOSPHOKETOLASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AEM9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTNPVIGTPW QKLDRPVSEE AIEGMDKYWR VANYMSIGQI YLRSNPLMKE
    60 70 80 90 100
    PFTRDDVKHR LVGHWGTTPG LNFLLAHINR LIADHQQNTV FIMGPGHGGP
    110 120 130 140 150
    AGTAQSYIDG TYTEYYPNIT KDEAGLQKFF RQFSYPGGIP SHFAPETPGS
    160 170 180 190 200
    IHEGGELGYA LSHAYGAIMD NPSLFVPCII GDGEAETGPL ATGWQSNKLV
    210 220 230 240 250
    NPRTDGIVLP ILHLNGYKIA NPTILARISD EELHDFFRGM GYHPYEFVAG
    260 270 280 290 300
    FDNEDHLSIH RRFAELFETI FDEICDIKAA AQTDDMTRPF YPMLIFRTPK
    310 320 330 340 350
    GWTCPKFIDG KKTEGSWRAH QVPLASARDT EAHFEVLKGW MESYKPEELF
    360 370 380 390 400
    NADGSIKEDV TAFMPKGELR IGANPNANGG RIREDLKLPE LDQYEITGVK
    410 420 430 440 450
    EYGHGWGQVE APRSLGAYCR DIIKNNPDSF RVFGPDETAS NRLNATYEVT
    460 470 480 490 500
    KKQWDNGYLS ALVDENMAVT GQVVEQLSEH QCEGFLEAYL LTGRHGIWSS
    510 520 530 540 550
    YESFVHVIDS MLNQHAKWLE ATVREIPWRK PISSVNLLVS SHVWRQDHNG
    560 570 580 590 600
    FSHQDPGVTS VLLNKTFNND HVTNIYFATD ANMLLAIAEK CFKSTNKINA
    610 620 630 640 650
    IFAGKQPAAT WITLDEVRAE LEAGAAEWKW ASNAKSNDEV QVVLAAAGDV
    660 670 680 690 700
    PTQEIMAASD ALNKMGIKFK VVNVVDLIKL QSSKENDEAM SDEDFADLFT
    710 720 730 740 750
    ADKPVLFAYH SYAQDVRGLI YDRPNHDNFT VVGYKEQGST TTPFDMVRVN
    760 770 780 790 800
    DMDRYALQAK ALELIDADKY ADKINELNEF RKTAFQFAVD NGYDIPEFTD
    810 820
    WVYPDVKVDE TSMLSATAAT AGDNE
    Length:825
    Mass (Da):92,530
    Last modified:June 1, 2001 - v1
    Checksum:iF1A418B84E7F97DF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181S → V AA sequence (PubMed:11292814).Curated
    Sequence conflicti23 – 231E → I AA sequence (PubMed:11292814).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ293946 Genomic DNA. Translation: CAC29121.1.
    CP001606 Genomic DNA. Translation: ACS47899.1.
    RefSeqiWP_012754421.1. NC_012815.1.

    Genome annotation databases

    EnsemblBacteriaiACS47899; ACS47899; Balat_0971.
    KEGGiblt:Balat_0971.
    PATRICi21111520. VBIBifAni93544_0969.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ293946 Genomic DNA. Translation: CAC29121.1.
    CP001606 Genomic DNA. Translation: ACS47899.1.
    RefSeqiWP_012754421.1. NC_012815.1.

    3D structure databases

    ProteinModelPortaliQ9AEM9.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiACS47899; ACS47899; Balat_0971.
    KEGGiblt:Balat_0971.
    PATRICi21111520. VBIBifAni93544_0969.

    Phylogenomic databases

    eggNOGiCOG3957.
    HOGENOMiHOG000236450.
    KOiK01621.
    OMAiVVKPRWP.
    OrthoDBiEOG61S2WD.

    Enzyme and pathway databases

    BioCyciBANI555970:GJ22-970-MONOMER.
    MetaCyc:MONOMER-12656.
    BRENDAi4.1.2.22. 6813.
    4.1.2.9. 6813.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    HAMAPiMF_01403. Phosphoketolase.
    InterProiIPR023962. Phosphoketolase.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005593. Xul5P/Fru6P_PKetolase.
    IPR018969. Xul5P/Fru6P_PKetolase_C.
    IPR019790. Xul5P/Fru6P_PKetolase_CS.
    IPR018970. Xul5P/Fru6P_PKetolase_N.
    IPR019789. Xul5P/Fru6P_PKetolase_ThDP_BS.
    [Graphical view]
    PfamiPF03894. XFP. 1 hit.
    PF09363. XFP_C. 1 hit.
    PF09364. XFP_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017245. Phosphoketolase. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    PROSITEiPS60002. PHOSPHOKETOLASE_1. 1 hit.
    PS60003. PHOSPHOKETOLASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Characterization of the D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase gene (xfp) from Bifidobacterium lactis."
      Meile L., Rohr L.M., Geissmann T.A., Herensperger M., Teuber M.
      J. Bacteriol. 183:2929-2936(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-31, CHARACTERIZATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 10140 / JCM 10602 / LMG 18314.

    Entry informationi

    Entry nameiXFP_BIFAS
    AccessioniPrimary (citable) accession number: Q9AEM9
    Secondary accession number(s): C6AIU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 14, 2001
    Last sequence update: June 1, 2001
    Last modified: July 22, 2015
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.