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Q9AEM1

- PCKG_CORGL

UniProt

Q9AEM1 - PCKG_CORGL

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi515 – 5184GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCGLU196627:GJDM-2845-MONOMER.
    BRENDAi4.1.1.32. 1648.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Synonyms:pck
    Ordered Locus Names:Cgl2863, cg3169
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103603Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi196627.cg3169.

    Structurei

    Secondary structure

    1
    610
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi18 – 3114
    Beta strandi34 – 385
    Helixi43 – 5513
    Beta strandi58 – 614
    Turni64 – 663
    Beta strandi71 – 733
    Helixi77 – 793
    Helixi84 – 863
    Beta strandi87 – 893
    Turni94 – 974
    Helixi106 – 11712
    Turni118 – 1236
    Beta strandi124 – 13411
    Beta strandi142 – 1498
    Helixi151 – 16010
    Beta strandi161 – 1644
    Helixi165 – 1717
    Turni172 – 1743
    Beta strandi178 – 1836
    Beta strandi204 – 2085
    Turni209 – 2124
    Beta strandi213 – 2186
    Helixi222 – 2254
    Helixi228 – 2347
    Helixi235 – 24410
    Beta strandi247 – 2493
    Beta strandi252 – 2576
    Beta strandi259 – 2613
    Beta strandi263 – 2697
    Beta strandi271 – 2733
    Helixi274 – 2807
    Beta strandi290 – 2978
    Beta strandi299 – 3035
    Beta strandi305 – 3117
    Beta strandi315 – 3206
    Turni326 – 3283
    Helixi330 – 3367
    Beta strandi342 – 3454
    Beta strandi347 – 3493
    Beta strandi389 – 3935
    Helixi394 – 3963
    Helixi402 – 4054
    Beta strandi413 – 4186
    Beta strandi422 – 4254
    Beta strandi427 – 4304
    Helixi434 – 4429
    Helixi464 – 4663
    Turni468 – 4703
    Helixi475 – 48915
    Helixi490 – 4923
    Beta strandi495 – 4995
    Beta strandi510 – 5123
    Helixi515 – 5173
    Helixi518 – 53013
    Beta strandi539 – 5435
    Helixi546 – 5483
    Helixi561 – 5655
    Helixi569 – 5746
    Helixi576 – 58510
    Helixi592 – 60514

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZCIX-ray2.30A/B/C/D1-610[»]
    ProteinModelPortaliQ9AEM1.
    SMRiQ9AEM1. Positions 13-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9AEM1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiIDWHGND.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AEM1-1 [UniParc]FASTAAdd to Basket

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    MTTAAIRGLQ GEAPTKNKEL LNWIADAVEL FQPEAVVFVD GSQAEWDRMA    50
    EDLVEAGTLI KLNEEKRPNS YLARSNPSDV ARVESRTFIC SEKEEDAGPT 100
    NNWAPPQAMK DEMSKHYAGS MKGRTMYVVP FCMGPISDPD PKLGVQLTDS 150
    EYVVMSMRIM TRMGIEALDK IGANGSFVRC LHSVGAPLEP GQEDVAWPCN 200
    DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH 250
    MLILKLINPE GKAYHIAAAF PSACGKTNLA MITPTIPGWT AQVVGDDIAW 300
    LKLREDGLYA VNPENGFFGV APGTNYASNP IAMKTMEPGN TLFTNVALTD 350
    DGDIWWEGMD GDAPAHLIDW MGNDWTPESD ENAAHPNSRY CVAIDQSPAA 400
    APEFNDWEGV KIDAILFGGR RADTVPLVTQ TYDWEHGTMV GALLASGQTA 450
    ASAEAKVGTL RHDPMAMLPF IGYNAGEYLQ NWIDMGNKGG DKMPSIFLVN 500
    WFRRGEDGRF LWPGFGDNSR VLKWVIDRIE GHVGADETVV GHTAKAEDLD 550
    LDGLDTPIED VKEALTAPAE QWANDVEDNA EYLTFLGPRV PAEVHSQFDA 600
    LKARISAAHA 610
    Length:610
    Mass (Da):66,874
    Last modified:June 1, 2001 - v1
    Checksum:i45C97817F49744E0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ269506 Genomic DNA. Translation: CAC36295.1.
    BA000036 Genomic DNA. Translation: BAC00257.1.
    BX927156 Genomic DNA. Translation: CAF20888.1.
    RefSeqiNP_602055.1. NC_003450.3.
    WP_011015446.1. NC_006958.1.
    YP_227104.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
    CAF20888; CAF20888; cg3169.
    GeneIDi1020806.
    KEGGicgb:cg3169.
    cgl:NCgl2765.
    PATRICi21497768. VBICorGlu203724_2795.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ269506 Genomic DNA. Translation: CAC36295.1 .
    BA000036 Genomic DNA. Translation: BAC00257.1 .
    BX927156 Genomic DNA. Translation: CAF20888.1 .
    RefSeqi NP_602055.1. NC_003450.3.
    WP_011015446.1. NC_006958.1.
    YP_227104.1. NC_006958.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZCI X-ray 2.30 A/B/C/D 1-610 [» ]
    ProteinModelPortali Q9AEM1.
    SMRi Q9AEM1. Positions 13-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg3169.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC00257 ; BAC00257 ; BAC00257 .
    CAF20888 ; CAF20888 ; cg3169 .
    GeneIDi 1020806.
    KEGGi cgb:cg3169.
    cgl:NCgl2765.
    PATRICi 21497768. VBICorGlu203724_2795.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi IDWHGND.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci CGLU196627:GJDM-2845-MONOMER.
    BRENDAi 4.1.1.32. 1648.

    Miscellaneous databases

    EvolutionaryTracei Q9AEM1.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production."
      Riedel C., Rittmann D., Dangel P., Mockel B., Petersen S., Sahm H., Eikmanns B.J.
      J. Mol. Microbiol. Biotechnol. 3:573-583(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. "Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum."
      Aich S., Prasad L., Delbaere L.T.J.
      Int. J. Biochem. Cell Biol. 40:1597-1603(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

    Entry informationi

    Entry nameiPCKG_CORGL
    AccessioniPrimary (citable) accession number: Q9AEM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3