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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.1 Publication

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi230ManganeseUniRule annotation1
Metal bindingi250Manganese; via tele nitrogenUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Active sitei274UniRule annotation1
Metal bindingi297ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 278GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-12481-MONOMER.
BRENDAi4.1.1.32. 960.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]1 PublicationUniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinase1 PublicationUniRule annotation
Short name:
PEPCK1 PublicationUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinase1 PublicationUniRule annotation
Short name:
GTP-PEPCK1 PublicationUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:Cgl2863, cg3169
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene lead to the absence of PEP carboxykinase activity and the inability to grow on acetate or lactate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036031 – 610Phosphoenolpyruvate carboxykinase [GTP]Add BLAST610

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi196627.cg3169.

Structurei

Secondary structure

1610
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 31Combined sources14
Beta strandi34 – 38Combined sources5
Helixi43 – 55Combined sources13
Beta strandi58 – 61Combined sources4
Turni64 – 66Combined sources3
Beta strandi71 – 73Combined sources3
Helixi77 – 79Combined sources3
Helixi84 – 86Combined sources3
Beta strandi87 – 89Combined sources3
Turni94 – 97Combined sources4
Helixi106 – 117Combined sources12
Turni118 – 123Combined sources6
Beta strandi124 – 134Combined sources11
Beta strandi142 – 149Combined sources8
Helixi151 – 160Combined sources10
Beta strandi161 – 164Combined sources4
Helixi165 – 171Combined sources7
Turni172 – 174Combined sources3
Beta strandi178 – 183Combined sources6
Beta strandi204 – 208Combined sources5
Turni209 – 212Combined sources4
Beta strandi213 – 218Combined sources6
Helixi222 – 225Combined sources4
Helixi228 – 234Combined sources7
Helixi235 – 244Combined sources10
Beta strandi247 – 249Combined sources3
Beta strandi252 – 257Combined sources6
Beta strandi259 – 261Combined sources3
Beta strandi263 – 269Combined sources7
Beta strandi271 – 273Combined sources3
Helixi274 – 280Combined sources7
Beta strandi290 – 297Combined sources8
Beta strandi299 – 303Combined sources5
Beta strandi305 – 311Combined sources7
Beta strandi315 – 320Combined sources6
Turni326 – 328Combined sources3
Helixi330 – 336Combined sources7
Beta strandi342 – 345Combined sources4
Beta strandi347 – 349Combined sources3
Beta strandi389 – 393Combined sources5
Helixi394 – 396Combined sources3
Helixi402 – 405Combined sources4
Beta strandi413 – 418Combined sources6
Beta strandi422 – 425Combined sources4
Beta strandi427 – 430Combined sources4
Helixi434 – 442Combined sources9
Helixi464 – 466Combined sources3
Turni468 – 470Combined sources3
Helixi475 – 489Combined sources15
Helixi490 – 492Combined sources3
Beta strandi495 – 499Combined sources5
Beta strandi510 – 512Combined sources3
Helixi515 – 517Combined sources3
Helixi518 – 530Combined sources13
Beta strandi539 – 543Combined sources5
Helixi546 – 548Combined sources3
Helixi561 – 565Combined sources5
Helixi569 – 574Combined sources6
Helixi576 – 585Combined sources10
Helixi592 – 605Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZCIX-ray2.30A/B/C/D1-610[»]
ProteinModelPortaliQ9AEM1.
SMRiQ9AEM1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AEM1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 223Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AEM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAAIRGLQ GEAPTKNKEL LNWIADAVEL FQPEAVVFVD GSQAEWDRMA
60 70 80 90 100
EDLVEAGTLI KLNEEKRPNS YLARSNPSDV ARVESRTFIC SEKEEDAGPT
110 120 130 140 150
NNWAPPQAMK DEMSKHYAGS MKGRTMYVVP FCMGPISDPD PKLGVQLTDS
160 170 180 190 200
EYVVMSMRIM TRMGIEALDK IGANGSFVRC LHSVGAPLEP GQEDVAWPCN
210 220 230 240 250
DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH
260 270 280 290 300
MLILKLINPE GKAYHIAAAF PSACGKTNLA MITPTIPGWT AQVVGDDIAW
310 320 330 340 350
LKLREDGLYA VNPENGFFGV APGTNYASNP IAMKTMEPGN TLFTNVALTD
360 370 380 390 400
DGDIWWEGMD GDAPAHLIDW MGNDWTPESD ENAAHPNSRY CVAIDQSPAA
410 420 430 440 450
APEFNDWEGV KIDAILFGGR RADTVPLVTQ TYDWEHGTMV GALLASGQTA
460 470 480 490 500
ASAEAKVGTL RHDPMAMLPF IGYNAGEYLQ NWIDMGNKGG DKMPSIFLVN
510 520 530 540 550
WFRRGEDGRF LWPGFGDNSR VLKWVIDRIE GHVGADETVV GHTAKAEDLD
560 570 580 590 600
LDGLDTPIED VKEALTAPAE QWANDVEDNA EYLTFLGPRV PAEVHSQFDA
610
LKARISAAHA
Length:610
Mass (Da):66,874
Last modified:June 1, 2001 - v1
Checksum:i45C97817F49744E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1.
BA000036 Genomic DNA. Translation: BAC00257.1.
BX927156 Genomic DNA. Translation: CAF20888.1.
RefSeqiNP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
CAF20888; CAF20888; cg3169.
GeneIDi1020806.
KEGGicgb:cg3169.
cgl:NCgl2765.
PATRICi21497768. VBICorGlu203724_2795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1.
BA000036 Genomic DNA. Translation: BAC00257.1.
BX927156 Genomic DNA. Translation: CAF20888.1.
RefSeqiNP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZCIX-ray2.30A/B/C/D1-610[»]
ProteinModelPortaliQ9AEM1.
SMRiQ9AEM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg3169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
CAF20888; CAF20888; cg3169.
GeneIDi1020806.
KEGGicgb:cg3169.
cgl:NCgl2765.
PATRICi21497768. VBICorGlu203724_2795.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciCORYNE:G18NG-12481-MONOMER.
BRENDAi4.1.1.32. 960.

Miscellaneous databases

EvolutionaryTraceiQ9AEM1.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_CORGL
AccessioniPrimary (citable) accession number: Q9AEM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The presence and the level of PEP carboxykinase activity has a strong influence on the biosynthesis of glutamate and a weak influence on the biosynthesis of lysine.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.