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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi515 – 5184GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2845-MONOMER.
BRENDAi4.1.1.32. 1648.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:Cgl2863, cg3169
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103603Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi196627.cg3169.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 3114Combined sources
Beta strandi34 – 385Combined sources
Helixi43 – 5513Combined sources
Beta strandi58 – 614Combined sources
Turni64 – 663Combined sources
Beta strandi71 – 733Combined sources
Helixi77 – 793Combined sources
Helixi84 – 863Combined sources
Beta strandi87 – 893Combined sources
Turni94 – 974Combined sources
Helixi106 – 11712Combined sources
Turni118 – 1236Combined sources
Beta strandi124 – 13411Combined sources
Beta strandi142 – 1498Combined sources
Helixi151 – 16010Combined sources
Beta strandi161 – 1644Combined sources
Helixi165 – 1717Combined sources
Turni172 – 1743Combined sources
Beta strandi178 – 1836Combined sources
Beta strandi204 – 2085Combined sources
Turni209 – 2124Combined sources
Beta strandi213 – 2186Combined sources
Helixi222 – 2254Combined sources
Helixi228 – 2347Combined sources
Helixi235 – 24410Combined sources
Beta strandi247 – 2493Combined sources
Beta strandi252 – 2576Combined sources
Beta strandi259 – 2613Combined sources
Beta strandi263 – 2697Combined sources
Beta strandi271 – 2733Combined sources
Helixi274 – 2807Combined sources
Beta strandi290 – 2978Combined sources
Beta strandi299 – 3035Combined sources
Beta strandi305 – 3117Combined sources
Beta strandi315 – 3206Combined sources
Turni326 – 3283Combined sources
Helixi330 – 3367Combined sources
Beta strandi342 – 3454Combined sources
Beta strandi347 – 3493Combined sources
Beta strandi389 – 3935Combined sources
Helixi394 – 3963Combined sources
Helixi402 – 4054Combined sources
Beta strandi413 – 4186Combined sources
Beta strandi422 – 4254Combined sources
Beta strandi427 – 4304Combined sources
Helixi434 – 4429Combined sources
Helixi464 – 4663Combined sources
Turni468 – 4703Combined sources
Helixi475 – 48915Combined sources
Helixi490 – 4923Combined sources
Beta strandi495 – 4995Combined sources
Beta strandi510 – 5123Combined sources
Helixi515 – 5173Combined sources
Helixi518 – 53013Combined sources
Beta strandi539 – 5435Combined sources
Helixi546 – 5483Combined sources
Helixi561 – 5655Combined sources
Helixi569 – 5746Combined sources
Helixi576 – 58510Combined sources
Helixi592 – 60514Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZCIX-ray2.30A/B/C/D1-610[»]
ProteinModelPortaliQ9AEM1.
SMRiQ9AEM1. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9AEM1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AEM1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTAAIRGLQ GEAPTKNKEL LNWIADAVEL FQPEAVVFVD GSQAEWDRMA
60 70 80 90 100
EDLVEAGTLI KLNEEKRPNS YLARSNPSDV ARVESRTFIC SEKEEDAGPT
110 120 130 140 150
NNWAPPQAMK DEMSKHYAGS MKGRTMYVVP FCMGPISDPD PKLGVQLTDS
160 170 180 190 200
EYVVMSMRIM TRMGIEALDK IGANGSFVRC LHSVGAPLEP GQEDVAWPCN
210 220 230 240 250
DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH
260 270 280 290 300
MLILKLINPE GKAYHIAAAF PSACGKTNLA MITPTIPGWT AQVVGDDIAW
310 320 330 340 350
LKLREDGLYA VNPENGFFGV APGTNYASNP IAMKTMEPGN TLFTNVALTD
360 370 380 390 400
DGDIWWEGMD GDAPAHLIDW MGNDWTPESD ENAAHPNSRY CVAIDQSPAA
410 420 430 440 450
APEFNDWEGV KIDAILFGGR RADTVPLVTQ TYDWEHGTMV GALLASGQTA
460 470 480 490 500
ASAEAKVGTL RHDPMAMLPF IGYNAGEYLQ NWIDMGNKGG DKMPSIFLVN
510 520 530 540 550
WFRRGEDGRF LWPGFGDNSR VLKWVIDRIE GHVGADETVV GHTAKAEDLD
560 570 580 590 600
LDGLDTPIED VKEALTAPAE QWANDVEDNA EYLTFLGPRV PAEVHSQFDA
610
LKARISAAHA
Length:610
Mass (Da):66,874
Last modified:June 1, 2001 - v1
Checksum:i45C97817F49744E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1.
BA000036 Genomic DNA. Translation: BAC00257.1.
BX927156 Genomic DNA. Translation: CAF20888.1.
RefSeqiNP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.
YP_227104.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
CAF20888; CAF20888; cg3169.
GeneIDi1020806.
3345234.
KEGGicgb:cg3169.
cgl:NCgl2765.
PATRICi21497768. VBICorGlu203724_2795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1.
BA000036 Genomic DNA. Translation: BAC00257.1.
BX927156 Genomic DNA. Translation: CAF20888.1.
RefSeqiNP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.
YP_227104.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZCIX-ray2.30A/B/C/D1-610[»]
ProteinModelPortaliQ9AEM1.
SMRiQ9AEM1. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg3169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
CAF20888; CAF20888; cg3169.
GeneIDi1020806.
3345234.
KEGGicgb:cg3169.
cgl:NCgl2765.
PATRICi21497768. VBICorGlu203724_2795.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciCGLU196627:GJDM-2845-MONOMER.
BRENDAi4.1.1.32. 1648.

Miscellaneous databases

EvolutionaryTraceiQ9AEM1.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production."
    Riedel C., Rittmann D., Dangel P., Mockel B., Petersen S., Sahm H., Eikmanns B.J.
    J. Mol. Microbiol. Biotechnol. 3:573-583(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. "Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum."
    Aich S., Prasad L., Delbaere L.T.J.
    Int. J. Biochem. Cell Biol. 40:1597-1603(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiPCKG_CORGL
AccessioniPrimary (citable) accession number: Q9AEM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.