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Q9AEM1

- PCKG_CORGL

UniProt

Q9AEM1 - PCKG_CORGL

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
pckG, pck, Cgl2863, cg3169
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821Substrate By similarity
Binding sitei223 – 2231Substrate; via amide nitrogen By similarity
Metal bindingi230 – 2301Manganese By similarity
Binding sitei230 – 2301Substrate By similarity
Metal bindingi250 – 2501Manganese; via tele nitrogen By similarity
Binding sitei272 – 2721Substrate By similarity
Active sitei274 – 2741
Metal bindingi297 – 2971Manganese By similarity
Binding sitei389 – 3891GTP By similarity
Binding sitei420 – 4201GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTP By similarity
Nucleotide bindingi515 – 5184GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2845-MONOMER.
BRENDAi4.1.1.32. 1648.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Synonyms:pck
Ordered Locus Names:Cgl2863, cg3169
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_0000103603Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi196627.cg3169.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 3114
Beta strandi34 – 385
Helixi43 – 5513
Beta strandi58 – 614
Turni64 – 663
Beta strandi71 – 733
Helixi77 – 793
Helixi84 – 863
Beta strandi87 – 893
Turni94 – 974
Helixi106 – 11712
Turni118 – 1236
Beta strandi124 – 13411
Beta strandi142 – 1498
Helixi151 – 16010
Beta strandi161 – 1644
Helixi165 – 1717
Turni172 – 1743
Beta strandi178 – 1836
Beta strandi204 – 2085
Turni209 – 2124
Beta strandi213 – 2186
Helixi222 – 2254
Helixi228 – 2347
Helixi235 – 24410
Beta strandi247 – 2493
Beta strandi252 – 2576
Beta strandi259 – 2613
Beta strandi263 – 2697
Beta strandi271 – 2733
Helixi274 – 2807
Beta strandi290 – 2978
Beta strandi299 – 3035
Beta strandi305 – 3117
Beta strandi315 – 3206
Turni326 – 3283
Helixi330 – 3367
Beta strandi342 – 3454
Beta strandi347 – 3493
Beta strandi389 – 3935
Helixi394 – 3963
Helixi402 – 4054
Beta strandi413 – 4186
Beta strandi422 – 4254
Beta strandi427 – 4304
Helixi434 – 4429
Helixi464 – 4663
Turni468 – 4703
Helixi475 – 48915
Helixi490 – 4923
Beta strandi495 – 4995
Beta strandi510 – 5123
Helixi515 – 5173
Helixi518 – 53013
Beta strandi539 – 5435
Helixi546 – 5483
Helixi561 – 5655
Helixi569 – 5746
Helixi576 – 58510
Helixi592 – 60514

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZCIX-ray2.30A/B/C/D1-610[»]
ProteinModelPortaliQ9AEM1.
SMRiQ9AEM1. Positions 13-606.

Miscellaneous databases

EvolutionaryTraceiQ9AEM1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiIDWHGND.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AEM1-1 [UniParc]FASTAAdd to Basket

« Hide

MTTAAIRGLQ GEAPTKNKEL LNWIADAVEL FQPEAVVFVD GSQAEWDRMA    50
EDLVEAGTLI KLNEEKRPNS YLARSNPSDV ARVESRTFIC SEKEEDAGPT 100
NNWAPPQAMK DEMSKHYAGS MKGRTMYVVP FCMGPISDPD PKLGVQLTDS 150
EYVVMSMRIM TRMGIEALDK IGANGSFVRC LHSVGAPLEP GQEDVAWPCN 200
DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH 250
MLILKLINPE GKAYHIAAAF PSACGKTNLA MITPTIPGWT AQVVGDDIAW 300
LKLREDGLYA VNPENGFFGV APGTNYASNP IAMKTMEPGN TLFTNVALTD 350
DGDIWWEGMD GDAPAHLIDW MGNDWTPESD ENAAHPNSRY CVAIDQSPAA 400
APEFNDWEGV KIDAILFGGR RADTVPLVTQ TYDWEHGTMV GALLASGQTA 450
ASAEAKVGTL RHDPMAMLPF IGYNAGEYLQ NWIDMGNKGG DKMPSIFLVN 500
WFRRGEDGRF LWPGFGDNSR VLKWVIDRIE GHVGADETVV GHTAKAEDLD 550
LDGLDTPIED VKEALTAPAE QWANDVEDNA EYLTFLGPRV PAEVHSQFDA 600
LKARISAAHA 610
Length:610
Mass (Da):66,874
Last modified:June 1, 2001 - v1
Checksum:i45C97817F49744E0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1.
BA000036 Genomic DNA. Translation: BAC00257.1.
BX927156 Genomic DNA. Translation: CAF20888.1.
RefSeqiNP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.
YP_227104.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00257; BAC00257; BAC00257.
CAF20888; CAF20888; cg3169.
GeneIDi1020806.
KEGGicgb:cg3169.
cgl:NCgl2765.
PATRICi21497768. VBICorGlu203724_2795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ269506 Genomic DNA. Translation: CAC36295.1 .
BA000036 Genomic DNA. Translation: BAC00257.1 .
BX927156 Genomic DNA. Translation: CAF20888.1 .
RefSeqi NP_602055.1. NC_003450.3.
WP_011015446.1. NC_006958.1.
YP_227104.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZCI X-ray 2.30 A/B/C/D 1-610 [» ]
ProteinModelPortali Q9AEM1.
SMRi Q9AEM1. Positions 13-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg3169.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC00257 ; BAC00257 ; BAC00257 .
CAF20888 ; CAF20888 ; cg3169 .
GeneIDi 1020806.
KEGGi cgb:cg3169.
cgl:NCgl2765.
PATRICi 21497768. VBICorGlu203724_2795.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi IDWHGND.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci CGLU196627:GJDM-2845-MONOMER.
BRENDAi 4.1.1.32. 1648.

Miscellaneous databases

EvolutionaryTracei Q9AEM1.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production."
    Riedel C., Rittmann D., Dangel P., Mockel B., Petersen S., Sahm H., Eikmanns B.J.
    J. Mol. Microbiol. Biotechnol. 3:573-583(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. "Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum."
    Aich S., Prasad L., Delbaere L.T.J.
    Int. J. Biochem. Cell Biol. 40:1597-1603(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiPCKG_CORGL
AccessioniPrimary (citable) accession number: Q9AEM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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