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Q9AE55

- MASZ_RHOFA

UniProt

Q9AE55 - MASZ_RHOFA

Protein

Malate synthase G

Gene

glcB

Organism
Rhodococcus fascians
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Synonyms:vicA
    OrganismiRhodococcus fascians
    Taxonomic identifieri1828 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 724724Malate synthase GPRO_0000166899Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AE55.
    SMRiQ9AE55. Positions 2-721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9AE55-1 [UniParc]FASTAAdd to Basket

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    MTDRVQAGGL QVAKVLFDFV EKEALPGTDL DSEAFWAGAA SVIADLAPKN    50
    KALLAVRDEI QGKVDAWHGE HAGAEYDRAA YKAFLKEIGY LLDEPADFQI 100
    HTSGVDTEIT TTAGPQLVVP VLNARFAINA SNARWGSLYD ALYGTDAIPE 150
    TDGAEKGTSY NKVRGDKVIA FARDFLDEAL PLSSGSHVGT TGYVVDAASL 200
    TVTLADGSTV GLKDPSQLLG YQGTPDAPTA ILFVHNGLHF EIQIDPESPI 250
    GKTDGAGVKD VLLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLMKGDL 300
    TEEVSKGGKT FTRAMNKDRT YTSVDGSELT LHGRSLLFVR NVGHLMTSDA 350
    ILDADGNEVP EGILDALFTS LAGLHSLTPD NVLSNSRTGS LYIVKPKMHG 400
    PDEVAFTAEL FGRVEQVLGL PTNTLKVGIM DEERRTTVNL KACIQAASER 450
    VVFINTGFLD RTGDEIHTSM EAGPVVRKGA MKGEKWIAAY EDFNVDTGLA 500
    AGLQGKAQIG KGMWAMPDLM HDMLEQKIGH PKAGANTAWV PSPTAATLHA 550
    LHYHKVDVFA RQHEIAKAKR ATVDEILEIP LAPSTDWTDE EKQNELDNNS 600
    QSILGYVVRW IDHGVGCSKV PDINDIALME DRATLRISSQ FIANWLRHGI 650
    VTEEQVRESL KRMAPVVDRQ NASDPTYKPL APDFDTNIAF QAASDLIFQG 700
    TSQPNGYTEP ILHRRRREYK AVNA 724
    Length:724
    Mass (Da):78,610
    Last modified:June 1, 2001 - v1
    Checksum:iF889FE883890995E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ301559 Genomic DNA. Translation: CAC35701.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ301559 Genomic DNA. Translation: CAC35701.1 .

    3D structure databases

    ProteinModelPortali Q9AE55.
    SMRi Q9AE55. Positions 2-721.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a chromosomal locus that affects pathogenicity in Rhodococcus fascians."
      Vereecke D.M., Cornelis K., Van Montagu M., El Jaziri M., Holsters M., Goethals K.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: D188.

    Entry informationi

    Entry nameiMASZ_RHOFA
    AccessioniPrimary (citable) accession number: Q9AE55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3