Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9AE55

- MASZ_RHOFA

UniProt

Q9AE55 - MASZ_RHOFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate synthase G

Gene

glcB

Organism
Rhodococcus fascians
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei631 – 6311Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Synonyms:vicA
OrganismiRhodococcus fascians
Taxonomic identifieri1828 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Malate synthase GPRO_0000166899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9AE55.
SMRiQ9AE55. Positions 2-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9AE55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRVQAGGL QVAKVLFDFV EKEALPGTDL DSEAFWAGAA SVIADLAPKN
60 70 80 90 100
KALLAVRDEI QGKVDAWHGE HAGAEYDRAA YKAFLKEIGY LLDEPADFQI
110 120 130 140 150
HTSGVDTEIT TTAGPQLVVP VLNARFAINA SNARWGSLYD ALYGTDAIPE
160 170 180 190 200
TDGAEKGTSY NKVRGDKVIA FARDFLDEAL PLSSGSHVGT TGYVVDAASL
210 220 230 240 250
TVTLADGSTV GLKDPSQLLG YQGTPDAPTA ILFVHNGLHF EIQIDPESPI
260 270 280 290 300
GKTDGAGVKD VLLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLMKGDL
310 320 330 340 350
TEEVSKGGKT FTRAMNKDRT YTSVDGSELT LHGRSLLFVR NVGHLMTSDA
360 370 380 390 400
ILDADGNEVP EGILDALFTS LAGLHSLTPD NVLSNSRTGS LYIVKPKMHG
410 420 430 440 450
PDEVAFTAEL FGRVEQVLGL PTNTLKVGIM DEERRTTVNL KACIQAASER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGPVVRKGA MKGEKWIAAY EDFNVDTGLA
510 520 530 540 550
AGLQGKAQIG KGMWAMPDLM HDMLEQKIGH PKAGANTAWV PSPTAATLHA
560 570 580 590 600
LHYHKVDVFA RQHEIAKAKR ATVDEILEIP LAPSTDWTDE EKQNELDNNS
610 620 630 640 650
QSILGYVVRW IDHGVGCSKV PDINDIALME DRATLRISSQ FIANWLRHGI
660 670 680 690 700
VTEEQVRESL KRMAPVVDRQ NASDPTYKPL APDFDTNIAF QAASDLIFQG
710 720
TSQPNGYTEP ILHRRRREYK AVNA
Length:724
Mass (Da):78,610
Last modified:June 1, 2001 - v1
Checksum:iF889FE883890995E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301559 Genomic DNA. Translation: CAC35701.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301559 Genomic DNA. Translation: CAC35701.1 .

3D structure databases

ProteinModelPortali Q9AE55.
SMRi Q9AE55. Positions 2-721.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of a chromosomal locus that affects pathogenicity in Rhodococcus fascians."
    Vereecke D.M., Cornelis K., Van Montagu M., El Jaziri M., Holsters M., Goethals K.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D188.

Entry informationi

Entry nameiMASZ_RHOFA
AccessioniPrimary (citable) accession number: Q9AE55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3