Q9AE11 (RUVC_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	Cgl1662, cg1871

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the RuvC family.
Sequence caution	The sequence CAF20044.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_0000183093
Sites
	Metal binding	12	1	Magnesium By similarity
	Metal binding	73	1	Magnesium By similarity
	Metal binding	146	1	Magnesium By similarity
	Metal binding	149	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AE11 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FA295C4D5704597D	FASTA	221	24,097
	10 20 30 40 50 60 MNHEGLRVMG IDPGLTRCGL SVVQAGRGRT VYPVSVGVVR TPPDAELAER LLRLSKAVGE 70 80 90 100 110 120 WMDEYTPDVI AIERVFERGN VSTVMNTAHA VGVLILAAAE RGLPVHMYTP SEVKKAISGN 130 140 150 160 170 180 GRADKKQMTV MITRILGLGE PPKPADAADA LSLAVCHCWR APMLMRAQSQ YSEQELEKRR 190 200 210 220 RVQQGKLGKA KSTYNAEQAQ SHASDPAKAA HPSQFQRTDT N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of Corynebacterium glutamicum secretion genes secD, secF and secG in transporting the Streptomyces griseus alpha-amylase." Berens S., Kalinowski J., Puehler A. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF038651 Genomic DNA. Translation: AAK19838.1. BA000036 Genomic DNA. Translation: BAB99055.1. BX927153 Genomic DNA. Translation: CAF20044.1. Different initiation.
RefSeq	NP_600874.2. NC_003450.3. YP_225945.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q9AE11.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg1871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB99055; BAB99055; BAB99055. CAF20044; CAF20044; cg1871.
GeneID	1019629. 3343449.
KEGG	cgb:cg1871. cgl:NCgl1598.
PATRIC	21495358. VBICorGlu203724_1622.
Phylogenomic databases
eggNOG	COG0817.
KO	K01159.
OMA	YTALQMK.
ProtClustDB	PRK00039.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-1643-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_CORGL

Accession

Primary (citable) accession number: Q9AE11

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program