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Q9AE11 (RUVC_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Cgl1662, cg1871
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence CAF20044.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 221221Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183093

Sites

Metal binding121Magnesium By similarity
Metal binding731Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding1491Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9AE11 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: FA295C4D5704597D

FASTA22124,097
        10         20         30         40         50         60 
MNHEGLRVMG IDPGLTRCGL SVVQAGRGRT VYPVSVGVVR TPPDAELAER LLRLSKAVGE 

        70         80         90        100        110        120 
WMDEYTPDVI AIERVFERGN VSTVMNTAHA VGVLILAAAE RGLPVHMYTP SEVKKAISGN 

       130        140        150        160        170        180 
GRADKKQMTV MITRILGLGE PPKPADAADA LSLAVCHCWR APMLMRAQSQ YSEQELEKRR 

       190        200        210        220 
RVQQGKLGKA KSTYNAEQAQ SHASDPAKAA HPSQFQRTDT N 

« Hide

References

« Hide 'large scale' references
[1]"The role of Corynebacterium glutamicum secretion genes secD, secF and secG in transporting the Streptomyces griseus alpha-amylase."
Berens S., Kalinowski J., Puehler A.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF038651 Genomic DNA. Translation: AAK19838.1.
BA000036 Genomic DNA. Translation: BAB99055.1.
BX927153 Genomic DNA. Translation: CAF20044.1. Different initiation.
RefSeqNP_600874.2. NC_003450.3.
YP_225945.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ9AE11.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99055; BAB99055; BAB99055.
CAF20044; CAF20044; cg1871.
GeneID1019629.
3343449.
KEGGcgb:cg1871.
cgl:NCgl1598.
PATRIC21495358. VBICorGlu203724_1622.

Phylogenomic databases

eggNOGCOG0817.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_CORGL
AccessionPrimary (citable) accession number: Q9AE11
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families