Q9ADI5 (Q9ADI5_STRCO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

SCO6078

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	244	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	281	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	379	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q9ADI5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8816DFF3BC1A3408	FASTA	581	63,952
	10 20 30 40 50 60 MQFEVWAPQA GRVTLRCDGA TRALERDPER PGWWCGEAPA RDGSRYGFAV DDGPVLPDPR 70 80 90 100 110 120 SRRQPDGPDG LSAVVDHGRY AWRTPWAGRP LPGGVLYELH VGTYTPEGTL DAAADRLEHL 130 140 150 160 170 180 VRLGVTHVEL MPLCPFPGRH GWGYEGVSLW AVHEPYGGPE ALKRFVDRAH DLGLGVVLDV 190 200 210 220 230 240 VHNHLGPSGN HLPAFGPYFT DTHHTPWGVA VNLDAPGSDE VRAYLVDSAL AWLRDYRLDG 250 260 270 280 290 300 LRLDAVHALA DTRACHFLEQ LSTAVDGLAA DLERPLFLIA ESDLNDPRII TPRAEGGLGV 310 320 330 340 350 360 HAQWNDDFHH ALHAALTGES QGYYADFARD PLAALAKTLI RGYFHDGTYS SFRGRSHGRA 370 380 390 400 410 420 LDRGRVAAHR LTGYSQTHDQ VGNRAQGDRL ASLVSPGLAA CAATLTLTAP FTPMLFMGEE 430 440 450 460 470 480 WAAGTPWQFF TDHTDPQLAQ AVRQGRRREF AAHGWREEDV PDPQDPATRE RSCLDWSEPE 490 500 510 520 530 540 REPHARVLDW YRRLIALRAE QPDLTDPDLA DTRVAYDGTG RWLAFRRGDI RVAVNLGTEP 550 560 570 580 AAIPLGNRPA RVLAAWEPVE GPGANGILRV PGESSVVLVQ E

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939126 Genomic DNA. Translation: CAC33923.1.
RefSeq	NP_630186.1. NC_003888.3.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ProteinModelPortal	Q9ADI5.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1101519.
GenomeReviews	Gene locus SCO6078 in contig AL645882_GR.
KEGG	sco:SCO6078.
NMPDR	fig|100226.1.peg.6023.
PATRIC	23742066. VBIStrCoe124346_6180.
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
PhylomeDB	Q9ADI5.
ProtClustDB	CLSK375663.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q9ADI5_STRCO

Accession

Primary (citable) accession number: Q9ADI5

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)