Reviewed, UniProtKB/Swiss-Prot Q9ADA4 (SYC2_STRCO)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	SCO1663
ORF Names:	SCI52.05c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159491
Regions
	Motif	286 – 290	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	289	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ADA4-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F0A2F56AD7C84579	FASTA	409	44,186
	10 20 30 40 50 60 MHAWPASEVP ALPGQGRDLR IHDTATGGPV TLDPGPVARI YVCGITPYDA THMGHAATYN 70 80 90 100 110 120 AFDLVQRVWL DTKRQVHYVQ NVTDVDDPLL ERAVRDGVDW TALAEQETAL FREDMTALRM 130 140 150 160 170 180 LPPQHYIGAV EAIPGIVPLV ERLRDAGAAY ELEGDVYFSV EADPHFGGVS HLDAATMRLL 190 200 210 220 230 240 SAERGGDPDR PGKKNPLDPM LWMAAREGEP SWDGGTLGRG RPGWHIECVA IALDHLGMGF 250 260 270 280 290 300 DVQGGGSDLA FPHHEMGASH AQALTGEFPM AKAYVHAGMV GLDGEKMSKS KGNLVFVSQL 310 320 330 340 350 360 RREGVDPAAI RLTLLAHHYR SDWEWTDQVL QDALARLDRW RAAVSRPDGP PAEALVEEIR 370 380 390 400 EALANDLDSP AALAAVDRWA ALQQESGGTD IGAPGVVSRA VDALLGVAL

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939109 Genomic DNA. Translation: CAC36366.1.
RefSeq	NP_625938.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	1097094.
GenomeReviews	Gene locus SCO1663 in contig AL645882_GR.
KEGG	sco:SCO1663.
NMPDR	fig|100226.1.peg.1630.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ADA4.
OMA	HYVQNVT.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO1663-MON.
BRENDA	6.1.1.16. 1084.
Family and domain databases
HAMAP	MF_00041. Divergent sequence. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR017812. Mycothiol_biosynthesis_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_STRCO

Accession

Primary (citable) accession number: Q9ADA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)