Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein CrtB/UppS

Gene

crtB/uppS3

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reaction from prephytoene diphosphate to phytoene.By similarity
Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.By similarity

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Pathway: phytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein CrtB/UppS (crtB/uppS3)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei329 – 3291By similarity
Metal bindingi329 – 3291MagnesiumBy similarity
Binding sitei334 – 3341SubstrateBy similarity
Binding sitei346 – 3461SubstrateBy similarity
Active sitei377 – 3771Proton acceptorBy similarity
Binding sitei378 – 3781SubstrateBy similarity
Binding sitei380 – 3801SubstrateBy similarity
Binding sitei497 – 4971SubstrateBy similarity
Metal bindingi515 – 5151MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein CrtB/UppS
Including the following 2 domains:
Phytoene synthase (EC:2.5.1.32)
Isoprenyl transferase (EC:2.5.1.-)
Gene namesi
Name:crtB/uppS3
Ordered Locus Names:SCP1.212
Encoded oniPlasmid SCP10 Publication
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973 Componenti: Plasmid SCP1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Bifunctional protein CrtB/UppSPRO_0000123687Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9ACU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni330 – 3334Substrate bindingBy similarity
Regioni374 – 3763Substrate bindingBy similarity
Regioni502 – 5043Substrate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the phytoene/squalene synthase family.Curated
In the C-terminal section; belongs to the UPP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0020.
HOGENOMiHOG000105666.
InParanoidiQ9ACU1.
OMAiFAVELWA.
OrthoDBiEOG68H89T.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
PF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
SSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
PS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ACU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRDWSACGS LLLVMTVAKD RGGPAVAGSG GGVESPELRA AYEVCEAEAR
60 70 80 90 100
QFAVELWAAA ETLPVETRPS LYAIASWSAY TDRIIDEGPL EGREERLAQW
110 120 130 140 150
SADTLADLRA GHSSHPLRRA LVDTVRRWGL AEALIEEHLD SARADCAAVP
160 170 180 190 200
VFETFKDQRR YLRGCSGALA ELWVPLLEPR GPEAFRLMSV LGEACQVADL
210 220 230 240 250
FEDLPDDLAA GRCYLPRQDL RGLGLDVDDL RRGEREEALN AFVDAQLAHW
260 270 280 290 300
RGLLEETVLA PSTVGARYQI FVHTLLLGAQ MHFDEVTLLR SRVLTQGLES
310 320 330 340 350
LVTGDGRMSR RAARPGPGPV PGHIAVIADG NRRWAEARGL LADQGHRAGI
360 370 380 390 400
RAVLRLVNAA QRTGIRHVTV YMFSTENWYR SQGEVAALFG AFADWFARGA
410 420 430 440 450
QTVHELGVRV RWSGRRDRLE ESLASSFELL ESMTANNDKL TLTICLDYGG
460 470 480 490 500
REELAAAARA LAAEAVAGTI RPEQIGMAEV ARHLYVPEMP DVDLLVRTCE
510 520 530 540 550
QRISNFLPWH LAYAELVFDP APWPDFDLAR LRDAVDSYAG RERRFGGDAE
560
LPAQAGNLEP ARNG
Length:564
Mass (Da):62,105
Last modified:June 1, 2001 - v1
Checksum:i88B1A330C1CC46C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589148 Genomic DNA. Translation: CAC36733.1.
RefSeqiNP_639818.1. NC_003903.1.
WP_011039510.1. NC_003903.1.
YP_009091117.1. NG_041665.1.

Genome annotation databases

EnsemblBacteriaiCAC36733; CAC36733; CAC36733.
GeneIDi1095290.
KEGGisco:SCP1.212.
PATRICi23746018. VBIStrCoe124346_8152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589148 Genomic DNA. Translation: CAC36733.1.
RefSeqiNP_639818.1. NC_003903.1.
WP_011039510.1. NC_003903.1.
YP_009091117.1. NG_041665.1.

3D structure databases

ProteinModelPortaliQ9ACU1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC36733; CAC36733; CAC36733.
GeneIDi1095290.
KEGGisco:SCP1.212.
PATRICi23746018. VBIStrCoe124346_8152.

Phylogenomic databases

eggNOGiCOG0020.
HOGENOMiHOG000105666.
InParanoidiQ9ACU1.
OMAiFAVELWA.
OrthoDBiEOG68H89T.

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
PF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
SSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
PS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiCRIT_STRCO
AccessioniPrimary (citable) accession number: Q9ACU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.