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Q9ACU1 (CRIT_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional protein CrtB/UppS

Including the following 2 domains:

  1. Phytoene synthase
    EC=2.5.1.32
  2. Isoprenyl transferase
    EC=2.5.1.-
Gene names
Name:crtB/uppS3
Ordered Locus Names:SCP1.212
Encoded onPlasmid SCP1
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reaction from prephytoene diphosphate to phytoene By similarity. HAMAP-Rule MF_01139

Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids By similarity. HAMAP-Rule MF_01139

Catalytic activity

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate. HAMAP-Rule MF_01139

Cofactor

Binds 2 magnesium ions per subunit By similarity.

Pathway

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1. HAMAP-Rule MF_01139

Subunit structure

Homodimer By similarity.

Sequence similarities

In the N-terminal section; belongs to the phytoene/squalene synthase family.

In the C-terminal section; belongs to the UPP synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Bifunctional protein CrtB/UppS HAMAP-Rule MF_01139
PRO_0000123687

Regions

Region330 – 3334Substrate binding By similarity
Region374 – 3763Substrate binding By similarity
Region502 – 5043Substrate binding By similarity

Sites

Active site3291 By similarity
Active site3771Proton acceptor By similarity
Metal binding3291Magnesium By similarity
Metal binding5151Magnesium By similarity
Binding site3341Substrate By similarity
Binding site3461Substrate By similarity
Binding site3781Substrate By similarity
Binding site3801Substrate By similarity
Binding site4971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ACU1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 88B1A330C1CC46C6

FASTA56462,105
        10         20         30         40         50         60 
MVRDWSACGS LLLVMTVAKD RGGPAVAGSG GGVESPELRA AYEVCEAEAR QFAVELWAAA 

        70         80         90        100        110        120 
ETLPVETRPS LYAIASWSAY TDRIIDEGPL EGREERLAQW SADTLADLRA GHSSHPLRRA 

       130        140        150        160        170        180 
LVDTVRRWGL AEALIEEHLD SARADCAAVP VFETFKDQRR YLRGCSGALA ELWVPLLEPR 

       190        200        210        220        230        240 
GPEAFRLMSV LGEACQVADL FEDLPDDLAA GRCYLPRQDL RGLGLDVDDL RRGEREEALN 

       250        260        270        280        290        300 
AFVDAQLAHW RGLLEETVLA PSTVGARYQI FVHTLLLGAQ MHFDEVTLLR SRVLTQGLES 

       310        320        330        340        350        360 
LVTGDGRMSR RAARPGPGPV PGHIAVIADG NRRWAEARGL LADQGHRAGI RAVLRLVNAA 

       370        380        390        400        410        420 
QRTGIRHVTV YMFSTENWYR SQGEVAALFG AFADWFARGA QTVHELGVRV RWSGRRDRLE 

       430        440        450        460        470        480 
ESLASSFELL ESMTANNDKL TLTICLDYGG REELAAAARA LAAEAVAGTI RPEQIGMAEV 

       490        500        510        520        530        540 
ARHLYVPEMP DVDLLVRTCE QRISNFLPWH LAYAELVFDP APWPDFDLAR LRDAVDSYAG 

       550        560 
RERRFGGDAE LPAQAGNLEP ARNG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL589148 Genomic DNA. Translation: CAC36733.1.
RefSeqNP_639818.1. NC_003903.1.

3D structure databases

ProteinModelPortalQ9ACU1.
ModBaseSearch...

Protein-protein interaction databases

STRING100226.SCP1.212.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC36733; CAC36733; CAC36733.
GeneID1095290.
KEGGsco:SCP1.212.
PATRIC23746018. VBIStrCoe124346_8152.

Phylogenomic databases

eggNOGCOG0020.
HOGENOMHOG000105666.
OMACEYLPAN.
ProtClustDBCLSK2755526.

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
3.40.1180.10. 1 hit.
HAMAPMF_01139. ISPT. Fused.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
IPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERPTHR10291. PTHR10291. 1 hit.
PfamPF01255. Prenyltransf. 1 hit.
PF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. Terpenoid_synth. 1 hit.
SSF64005. UPP_synth. 1 hit.
TIGRFAMsTIGR00055. uppS. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. False negative.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
PS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRIT_STRCO
AccessionPrimary (citable) accession number: Q9ACU1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: June 1, 2001
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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