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Q9AC32

- PNP_CAUCR

UniProt

Q9AC32 - PNP_CAUCR

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi488 – 4881MagnesiumUniRule annotation
    Metal bindingi494 – 4941MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCAULO:CC0034-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CC_0034
    OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
    Taxonomic identifieri190650 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
    ProteomesiUP000001816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 712712Polyribonucleotide nucleotidyltransferasePRO_0000329577Add
    BLAST

    Structurei

    Secondary structure

    1
    712
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 63
    Beta strandi14 – 2310
    Beta strandi27 – 359
    Beta strandi38 – 469
    Beta strandi59 – 657
    Helixi67 – 704
    Beta strandi76 – 783
    Helixi86 – 9914
    Helixi100 – 1023
    Beta strandi111 – 1199
    Beta strandi122 – 1243
    Helixi126 – 14116
    Beta strandi150 – 1578
    Beta strandi160 – 1645
    Helixi167 – 1715
    Beta strandi174 – 1818
    Beta strandi186 – 19611
    Helixi198 – 21114
    Helixi213 – 22614
    Helixi239 – 25820
    Helixi262 – 27716
    Turni278 – 2814
    Beta strandi292 – 2943
    Helixi295 – 31218
    Beta strandi313 – 3164
    Beta strandi329 – 3335
    Beta strandi339 – 3479
    Beta strandi350 – 35910
    Helixi361 – 3633
    Beta strandi364 – 3696
    Beta strandi372 – 3765
    Beta strandi378 – 3836
    Helixi386 – 3894
    Helixi400 – 41314
    Helixi414 – 4163
    Turni420 – 4223
    Beta strandi426 – 43510
    Helixi440 – 45415
    Beta strandi464 – 4729
    Beta strandi477 – 4826
    Helixi487 – 4904
    Beta strandi491 – 50010
    Beta strandi503 – 5119
    Helixi518 – 54023
    Beta strandi552 – 5543
    Beta strandi556 – 5616
    Helixi564 – 5663
    Helixi567 – 5715
    Helixi573 – 5753
    Helixi576 – 58510
    Beta strandi588 – 5914
    Beta strandi593 – 60311
    Helixi604 – 6129
    Beta strandi627 – 6359
    Beta strandi638 – 6469
    Beta strandi649 – 65911
    Turni662 – 6643
    Beta strandi673 – 6808
    Turni683 – 6853
    Beta strandi688 – 6903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AIDX-ray2.60A/B/C1-712[»]
    4AIMX-ray3.30A1-712[»]
    4AM3X-ray3.00A/B/C1-712[»]
    ProteinModelPortaliQ9AC32.
    SMRiQ9AC32. Positions 1-696.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 61460KHUniRule annotationAdd
    BLAST
    Domaini624 – 69269S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AC32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDIKRKTIE WGGKTLVLET GRIARQADGA VLATMGETVV LATAVFAKSQ    50
    KPGQDFFPLT VNYQEKTFAA GKIPGGFFKR EGRPSEKETL VSRLIDRPIR 100
    PLFVKGFKNE VQVVVTVLQH DLENDPDILG MVAASAALCL SGAPFMGPIG 150
    AARVGWVDGA YVLNPTLDEM KESKMDLVVA GTADAVMMVE SEIQELSEEI 200
    VLGGVNFAHQ QMQAVIDAII DLAEHAAKEP FAFEPEDTDA IKAKMKDLVG 250
    ADIAAAYKIQ KKQDRYEAVG AAKKKAIAAL GLSDENPTGY DPLKLGAIFK 300
    ELEADVVRRG ILDTGLRIDG RDVKTVRPIL GEVGILPRTH GSALFTRGET 350
    QAIVVATLGT GDDEQFIDAL EGTYKESFLL HYNFPPYSVG ETGRMGSPGR 400
    REIGHGKLAW RALRPMLPTK EDFPYTIRLV SEITESNGSS SMATVCGSSL 450
    AMMDAGVPLV RPVSGIAMGL ILEQDGFAVL SDILGDEDHL GDMDFKVAGT 500
    SEGLTSLQMD IKIAGITPAI MEQALAQAKE GRAHILGEMN KAMDAPRADV 550
    GDFAPKIETI NIPTDKIREV IGSGGKVIRE IVATTGAKVD INDDGVVKVS 600
    ASDGAKIKAA IDWIKSITDE AEVGKIYDGK VVKVVDFGAF VNFFGAKDGL 650
    VHVSQISNER VAKPSDVLKE GQMVKVKLLG FDDRGKTKLS MKVVDQETGE 700
    DLSKKEAAAE EA 712
    Length:712
    Mass (Da):76,448
    Last modified:June 1, 2001 - v1
    Checksum:i4772C4229037CA96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005673 Genomic DNA. Translation: AAK22022.1.
    PIRiB87253.
    RefSeqiNP_418854.1. NC_002696.2.
    WP_010917924.1. NC_002696.2.

    Genome annotation databases

    EnsemblBacteriaiAAK22022; AAK22022; CC_0034.
    GeneIDi944094.
    KEGGiccr:CC_0034.
    PATRICi21296988. VBICauCre124313_0034.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005673 Genomic DNA. Translation: AAK22022.1 .
    PIRi B87253.
    RefSeqi NP_418854.1. NC_002696.2.
    WP_010917924.1. NC_002696.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AID X-ray 2.60 A/B/C 1-712 [» ]
    4AIM X-ray 3.30 A 1-712 [» ]
    4AM3 X-ray 3.00 A/B/C 1-712 [» ]
    ProteinModelPortali Q9AC32.
    SMRi Q9AC32. Positions 1-696.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK22022 ; AAK22022 ; CC_0034 .
    GeneIDi 944094.
    KEGGi ccr:CC_0034.
    PATRICi 21296988. VBICauCre124313_0034.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CAULO:CC0034-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19089 / CB15.

    Entry informationi

    Entry nameiPNP_CAUCR
    AccessioniPrimary (citable) accession number: Q9AC32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3