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Q9AC32

- PNP_CAUCR

UniProt

Q9AC32 - PNP_CAUCR

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881MagnesiumUniRule annotation
Metal bindingi494 – 4941MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCAULO:CC0034-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:CC_0034
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712Polyribonucleotide nucleotidyltransferasePRO_0000329577Add
BLAST

Structurei

Secondary structure

1
712
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi14 – 2310Combined sources
Beta strandi27 – 359Combined sources
Beta strandi38 – 469Combined sources
Beta strandi59 – 657Combined sources
Helixi67 – 704Combined sources
Beta strandi76 – 783Combined sources
Helixi86 – 9914Combined sources
Helixi100 – 1023Combined sources
Beta strandi111 – 1199Combined sources
Beta strandi122 – 1243Combined sources
Helixi126 – 14116Combined sources
Beta strandi150 – 1578Combined sources
Beta strandi160 – 1645Combined sources
Helixi167 – 1715Combined sources
Beta strandi174 – 1818Combined sources
Beta strandi186 – 19611Combined sources
Helixi198 – 21114Combined sources
Helixi213 – 22614Combined sources
Helixi239 – 25820Combined sources
Helixi262 – 27716Combined sources
Turni278 – 2814Combined sources
Beta strandi292 – 2943Combined sources
Helixi295 – 31218Combined sources
Beta strandi313 – 3164Combined sources
Beta strandi329 – 3335Combined sources
Beta strandi339 – 3479Combined sources
Beta strandi350 – 35910Combined sources
Helixi361 – 3633Combined sources
Beta strandi364 – 3696Combined sources
Beta strandi372 – 3765Combined sources
Beta strandi378 – 3836Combined sources
Helixi386 – 3894Combined sources
Helixi400 – 41314Combined sources
Helixi414 – 4163Combined sources
Turni420 – 4223Combined sources
Beta strandi426 – 43510Combined sources
Helixi440 – 45415Combined sources
Beta strandi464 – 4729Combined sources
Beta strandi477 – 4826Combined sources
Helixi487 – 4904Combined sources
Beta strandi491 – 50010Combined sources
Beta strandi503 – 5119Combined sources
Helixi518 – 54023Combined sources
Beta strandi552 – 5543Combined sources
Beta strandi556 – 5616Combined sources
Helixi564 – 5663Combined sources
Helixi567 – 5715Combined sources
Helixi573 – 5753Combined sources
Helixi576 – 58510Combined sources
Beta strandi588 – 5914Combined sources
Beta strandi593 – 60311Combined sources
Helixi604 – 6129Combined sources
Beta strandi627 – 6359Combined sources
Beta strandi638 – 6469Combined sources
Beta strandi649 – 65911Combined sources
Turni662 – 6643Combined sources
Beta strandi673 – 6808Combined sources
Turni683 – 6853Combined sources
Beta strandi688 – 6903Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AIDX-ray2.60A/B/C1-712[»]
4AIMX-ray3.30A1-712[»]
4AM3X-ray3.00A/B/C1-712[»]
ProteinModelPortaliQ9AC32.
SMRiQ9AC32. Positions 1-696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KHUniRule annotationAdd
BLAST
Domaini624 – 69269S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AC32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDIKRKTIE WGGKTLVLET GRIARQADGA VLATMGETVV LATAVFAKSQ
60 70 80 90 100
KPGQDFFPLT VNYQEKTFAA GKIPGGFFKR EGRPSEKETL VSRLIDRPIR
110 120 130 140 150
PLFVKGFKNE VQVVVTVLQH DLENDPDILG MVAASAALCL SGAPFMGPIG
160 170 180 190 200
AARVGWVDGA YVLNPTLDEM KESKMDLVVA GTADAVMMVE SEIQELSEEI
210 220 230 240 250
VLGGVNFAHQ QMQAVIDAII DLAEHAAKEP FAFEPEDTDA IKAKMKDLVG
260 270 280 290 300
ADIAAAYKIQ KKQDRYEAVG AAKKKAIAAL GLSDENPTGY DPLKLGAIFK
310 320 330 340 350
ELEADVVRRG ILDTGLRIDG RDVKTVRPIL GEVGILPRTH GSALFTRGET
360 370 380 390 400
QAIVVATLGT GDDEQFIDAL EGTYKESFLL HYNFPPYSVG ETGRMGSPGR
410 420 430 440 450
REIGHGKLAW RALRPMLPTK EDFPYTIRLV SEITESNGSS SMATVCGSSL
460 470 480 490 500
AMMDAGVPLV RPVSGIAMGL ILEQDGFAVL SDILGDEDHL GDMDFKVAGT
510 520 530 540 550
SEGLTSLQMD IKIAGITPAI MEQALAQAKE GRAHILGEMN KAMDAPRADV
560 570 580 590 600
GDFAPKIETI NIPTDKIREV IGSGGKVIRE IVATTGAKVD INDDGVVKVS
610 620 630 640 650
ASDGAKIKAA IDWIKSITDE AEVGKIYDGK VVKVVDFGAF VNFFGAKDGL
660 670 680 690 700
VHVSQISNER VAKPSDVLKE GQMVKVKLLG FDDRGKTKLS MKVVDQETGE
710
DLSKKEAAAE EA
Length:712
Mass (Da):76,448
Last modified:June 1, 2001 - v1
Checksum:i4772C4229037CA96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK22022.1.
PIRiB87253.
RefSeqiNP_418854.1. NC_002696.2.
WP_010917924.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK22022; AAK22022; CC_0034.
GeneIDi944094.
KEGGiccr:CC_0034.
PATRICi21296988. VBICauCre124313_0034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK22022.1 .
PIRi B87253.
RefSeqi NP_418854.1. NC_002696.2.
WP_010917924.1. NC_002696.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AID X-ray 2.60 A/B/C 1-712 [» ]
4AIM X-ray 3.30 A 1-712 [» ]
4AM3 X-ray 3.00 A/B/C 1-712 [» ]
ProteinModelPortali Q9AC32.
SMRi Q9AC32. Positions 1-696.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK22022 ; AAK22022 ; CC_0034 .
GeneIDi 944094.
KEGGi ccr:CC_0034.
PATRICi 21296988. VBICauCre124313_0034.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CAULO:CC0034-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiPNP_CAUCR
AccessioniPrimary (citable) accession number: Q9AC32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3