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Q9AC32

- PNP_CAUCR

UniProt

Q9AC32 - PNP_CAUCR

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, CC_0034
Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881Magnesium By similarity
Metal bindingi494 – 4941Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCAULO:CC0034-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CC_0034
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001816: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329577Add
BLAST

Structurei

Secondary structure

1
712
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63
Beta strandi14 – 2310
Beta strandi27 – 359
Beta strandi38 – 469
Beta strandi59 – 657
Helixi67 – 704
Beta strandi76 – 783
Helixi86 – 9914
Helixi100 – 1023
Beta strandi111 – 1199
Beta strandi122 – 1243
Helixi126 – 14116
Beta strandi150 – 1578
Beta strandi160 – 1645
Helixi167 – 1715
Beta strandi174 – 1818
Beta strandi186 – 19611
Helixi198 – 21114
Helixi213 – 22614
Helixi239 – 25820
Helixi262 – 27716
Turni278 – 2814
Beta strandi292 – 2943
Helixi295 – 31218
Beta strandi313 – 3164
Beta strandi329 – 3335
Beta strandi339 – 3479
Beta strandi350 – 35910
Helixi361 – 3633
Beta strandi364 – 3696
Beta strandi372 – 3765
Beta strandi378 – 3836
Helixi386 – 3894
Helixi400 – 41314
Helixi414 – 4163
Turni420 – 4223
Beta strandi426 – 43510
Helixi440 – 45415
Beta strandi464 – 4729
Beta strandi477 – 4826
Helixi487 – 4904
Beta strandi491 – 50010
Beta strandi503 – 5119
Helixi518 – 54023
Beta strandi552 – 5543
Beta strandi556 – 5616
Helixi564 – 5663
Helixi567 – 5715
Helixi573 – 5753
Helixi576 – 58510
Beta strandi588 – 5914
Beta strandi593 – 60311
Helixi604 – 6129
Beta strandi627 – 6359
Beta strandi638 – 6469
Beta strandi649 – 65911
Turni662 – 6643
Beta strandi673 – 6808
Turni683 – 6853
Beta strandi688 – 6903

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AIDX-ray2.60A/B/C1-712[»]
4AIMX-ray3.30A1-712[»]
4AM3X-ray3.00A/B/C1-712[»]
ProteinModelPortaliQ9AC32.
SMRiQ9AC32. Positions 1-696.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KHAdd
BLAST
Domaini624 – 69269S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AC32-1 [UniParc]FASTAAdd to Basket

« Hide

MFDIKRKTIE WGGKTLVLET GRIARQADGA VLATMGETVV LATAVFAKSQ    50
KPGQDFFPLT VNYQEKTFAA GKIPGGFFKR EGRPSEKETL VSRLIDRPIR 100
PLFVKGFKNE VQVVVTVLQH DLENDPDILG MVAASAALCL SGAPFMGPIG 150
AARVGWVDGA YVLNPTLDEM KESKMDLVVA GTADAVMMVE SEIQELSEEI 200
VLGGVNFAHQ QMQAVIDAII DLAEHAAKEP FAFEPEDTDA IKAKMKDLVG 250
ADIAAAYKIQ KKQDRYEAVG AAKKKAIAAL GLSDENPTGY DPLKLGAIFK 300
ELEADVVRRG ILDTGLRIDG RDVKTVRPIL GEVGILPRTH GSALFTRGET 350
QAIVVATLGT GDDEQFIDAL EGTYKESFLL HYNFPPYSVG ETGRMGSPGR 400
REIGHGKLAW RALRPMLPTK EDFPYTIRLV SEITESNGSS SMATVCGSSL 450
AMMDAGVPLV RPVSGIAMGL ILEQDGFAVL SDILGDEDHL GDMDFKVAGT 500
SEGLTSLQMD IKIAGITPAI MEQALAQAKE GRAHILGEMN KAMDAPRADV 550
GDFAPKIETI NIPTDKIREV IGSGGKVIRE IVATTGAKVD INDDGVVKVS 600
ASDGAKIKAA IDWIKSITDE AEVGKIYDGK VVKVVDFGAF VNFFGAKDGL 650
VHVSQISNER VAKPSDVLKE GQMVKVKLLG FDDRGKTKLS MKVVDQETGE 700
DLSKKEAAAE EA 712
Length:712
Mass (Da):76,448
Last modified:June 1, 2001 - v1
Checksum:i4772C4229037CA96
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005673 Genomic DNA. Translation: AAK22022.1.
PIRiB87253.
RefSeqiNP_418854.1. NC_002696.2.
WP_010917924.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK22022; AAK22022; CC_0034.
GeneIDi944094.
KEGGiccr:CC_0034.
PATRICi21296988. VBICauCre124313_0034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005673 Genomic DNA. Translation: AAK22022.1 .
PIRi B87253.
RefSeqi NP_418854.1. NC_002696.2.
WP_010917924.1. NC_002696.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AID X-ray 2.60 A/B/C 1-712 [» ]
4AIM X-ray 3.30 A 1-712 [» ]
4AM3 X-ray 3.00 A/B/C 1-712 [» ]
ProteinModelPortali Q9AC32.
SMRi Q9AC32. Positions 1-696.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK22022 ; AAK22022 ; CC_0034 .
GeneIDi 944094.
KEGGi ccr:CC_0034.
PATRICi 21296988. VBICauCre124313_0034.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CAULO:CC0034-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiPNP_CAUCR
AccessioniPrimary (citable) accession number: Q9AC32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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