Reviewed, UniProtKB/Swiss-Prot Q9ABY4 (PUR9_CAUCR)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	CC_0086

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192081

Sequences

	Sequence		Length	Mass (Da)
	Q9ABY4-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 86196441DCD993D9	FASTA	529	55,238
	10 20 30 40 50 60 MPAAPDYPSA PDLVAPKRAL LSVSDKTGLV DAAKILHAAG VELVSTGGTK AAIAAAGVPV 70 80 90 100 110 120 KDVSDLTGFP EMMDGRVKTL HPVVHGGLLG VRDAADHAKA MADHGIGGID ILYVNLYPFE 130 140 150 160 170 180 ATVAKGGSYA ECVENIDIGG PAMIRSAAKN HGYVAVCTDP SDLAEVLEAL KAGGTTLALR 190 200 210 220 230 240 QQLAARAYAR TAAYDAAISA WFAEALGQDF PARKSIAGTL RQTMRYGENP HQKAAFYTFP 250 260 270 280 290 300 NPRPGVATAT QLQGKELSYN NINDTDAAFE LIAEFDPAAG PAVAIIKHAN PCGVAVGATQ 310 320 330 340 350 360 REAYERALAC DPTSAFGGIV AVNSRLTREA AEAMVEIFTE VVIAPEADED AIAVFAAKKN 370 380 390 400 410 420 LRLLVTGGLP DALSTGDTFK SVAGGFLVQS RDDARIKASD LKIVTQRQPT EEEVRDMLFA 430 440 450 460 470 480 FTVGKHVKSN AIVYARAGQT LGVGAGQMNR KDSARIAALR AADFGLDLKG CACASEAFFP 490 500 510 520 FADGLIQAAE AGATAIIQPG GSMRDPEVIE AADKLGLTMA FTGVRVFRH

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK22073.1.
PIR	E87259.
RefSeq	NP_418905.1.
3D structure databases
HSSP	HSSP built from PDB template 1G8M based on UniProtKB P31335.
ModBase	Search...
Genome annotation databases
GeneID	944147.
GenomeReviews	Gene locus CC_0086 in contig AE005673_GR.
KEGG	ccr:CC_0086.
NMPDR	fig|190650.1.peg.85.
TIGR	CC_0086.
Phylogenomic databases
HOGENOM	Q9ABY4.
OMA	Q9ABY4. VVKHVKS.
Enzyme and pathway databases
BRENDA	2.1.2.3. 2191. 3.5.4.10. 2191.
Family and domain databases
HAMAP	MF_00139. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CAUCR

Accession

Primary (citable) accession number: Q9ABY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)