Reviewed, UniProtKB/Swiss-Prot Q9ABN3 (LEU3_CAUCR)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	CC_0193

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	3-isopropylmalate dehydrogenase HAMAP MF_01033		PRO_0000083677
Regions
	Nucleotide binding	71 – 84	14	NAD By similarity
	Nucleotide binding	279 – 291	13	NAD By similarity
Sites
	Metal binding	220	1	Magnesium or manganese By similarity
	Metal binding	244	1	Magnesium or manganese By similarity
	Metal binding	248	1	Magnesium or manganese By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
	Site	136	1	Important for catalysis By similarity
	Site	187	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ABN3-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 0C5748110F9DE506	FASTA	350	37,012
	10 20 30 40 50 60 MATLLLLPGD GIGPEVCAQV RRVAAALTPD LKVDEALYGG ASYDTHGTPL TDEVREQALA 70 80 90 100 110 120 SDAVLMGAVG GPKWADAPRH LRPEAGLLNL RKAMDVFANL RPAYCFEALA GASSLKPELV 130 140 150 160 170 180 SGLDIMFVRE LVGGVYFGQP RGIEDLADGQ KKGFDTQVYT TSEIERVGRV AFELARGRTN 190 200 210 220 230 240 KVHSAEKSNV MESGLLWKQV ITELHAREYP DVQLEHILAD NCAMQLVRAP KQFDVIVTDN 250 260 270 280 290 300 LFGDILSDAA AMLTGSLGML PSAALGAPGK PGLYEPIHGS APDIAGKGLA NPLAAILSFE 310 320 330 340 350 MALRWSLKQT EAADALLAAV KAALDNGART RDLGGSLTTT QMGDAVLAAL

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK22180.1.
PIR	H87272.
RefSeq	NP_419012.1.
3D structure databases
HSSP	HSSP built from PDB template 1A05 based on UniProtKB Q56268.
ModBase	Search...
Genome annotation databases
GeneID	944254.
GenomeReviews	Gene locus CC_0193 in contig AE005673_GR.
KEGG	ccr:CC_0193.
NMPDR	fig|190650.1.peg.192.
TIGR	CC_0193.
Phylogenomic databases
HOGENOM	Q9ABN3.
OMA	Q9ABN3. EAFDTMR.
Enzyme and pathway databases
BRENDA	1.1.1.85. 2191.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CAUCR

Accession

Primary (citable) accession number: Q9ABN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)