Reviewed, UniProtKB/Swiss-Prot Q9ABH0 (SAHH_CAUCR)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	CC_0257

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116955
Regions
	Region	216 – 381	166	NAD binding By similarity
Sites
	Binding site	54	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	219	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ABH0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 0880060406FD9EF9	FASTA	463	50,761
	10 20 30 40 50 60 MADYIVKDIS LADFGRKEIA IAETEMPGLM ATRAEYGPQQ ILKGARIAGS LHMTIQTAVL 70 80 90 100 110 120 IETLTALGAE VRWASCNIFS TQDHAAAAIA AAGVPVFAFK GENLVEYWEY AHKIFEWHDG 130 140 150 160 170 180 GYPNLILDDG GDATLLCVLG PKAEKDPSIL NNPQNEEEEA LYAVMKKYLA EKPGFYSAIR 190 200 210 220 230 240 AAIGGVSEET TTGVHRLYQM AQKDELPFPA INVNDSVTKS KFDNLYGCRE SLVDAIRRGT 250 260 270 280 290 300 DVMLSGKVAV VCGYGDVGKG SAASLRQGGA RVIVTEVDPI CALQAAMEGY EVQTLNDVAD 310 320 330 340 350 360 KADIFVTATG NKDVITVDDM RKMKNNAIVC NIGHFDSEIQ IAGLRNFKWD EIKPQVHHVE 370 380 390 400 410 420 FPDGKKLIVL SEGRLVNLGN ATGHPSFVMS ASFTNQTLAQ IELWTNKAKY ENQVYTLPKH 430 440 450 460 LDEKVAFLHL EKLGAKLTTL RKDQADYIGV PEAGPFKPDH YRY

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK22244.1.
PIR	H87280.
RefSeq	NP_419076.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3R based on UniProtKB P10760.
ModBase	Search...
Genome annotation databases
GeneID	942403.
GenomeReviews	Gene locus CC_0257 in contig AE005673_GR.
KEGG	ccr:CC_0257.
NMPDR	fig|190650.1.peg.256.
TIGR	CC_0257.
Phylogenomic databases
HOGENOM	Q9ABH0.
OMA	HMRAMKD.
Enzyme and pathway databases
BRENDA	3.3.1.1. 2191.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CAUCR

Accession

Primary (citable) accession number: Q9ABH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)