Reviewed, UniProtKB/Swiss-Prot Q9ABD2 (PUR2_CAUCR)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	CC_0297

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151441
Regions
	Domain	110 – 315	206	ATP-grasp
	Nucleotide binding	136 – 196	61	ATP By similarity
Sites
	Metal binding	285	1	Magnesium or manganese By similarity
	Metal binding	287	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ABD2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 426ABF4E04E3378C	FASTA	427	44,265
	10 20 30 40 50 60 MEKLTILLVG SGGREHALAW KIAQSPLCGR LVAAPGNPGI EAVAELRAVK ATDADGLVAL 70 80 90 100 110 120 AQEIGADLVV VGPESALEVG LADKLAAVGI PCFGGSQRAA QLETSKAFTK DFCQRHGLPT 130 140 150 160 170 180 AAYGVFEDAA SAGAFLDTLE APFVIKADGL AAGKGVVIAP DRAAADAAVV DMLGGRFGSA 190 200 210 220 230 240 GARVVIEEFM HGEEASLFAV CDGKTAVLFG AAQDHKRAYD GDEGPNTGGM GTYSPPPVLT 250 260 270 280 290 300 DALIDQAWRE LIVPTVEGMA AEGNPYVGVL YAGLMLTPTG PKLVEYNARF GDPECQTLML 310 320 330 340 350 360 RLDSDIVPIL LAAAKGELAN AEPPKWREEA AICVVLAAEG YPDAPKTGGR IQGADADFGD 370 380 390 400 410 420 DVVVFHAGTT REFEGRLVAS GGRVLNVCAL GATLSEAREA AYGALETISL EGGFYRTDIG WRALQQR

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK22284.1.
PIR	H87285.
RefSeq	NP_419116.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	942342.
GenomeReviews	Gene locus CC_0297 in contig AE005673_GR.
KEGG	ccr:CC_0297.
NMPDR	fig|190650.1.peg.296.
TIGR	CC_0297.
Phylogenomic databases
HOGENOM	Q9ABD2.
OMA	IEYNCRF.
Enzyme and pathway databases
BRENDA	6.3.4.13. 2191.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CAUCR

Accession

Primary (citable) accession number: Q9ABD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)